ID   ANFA_AZOVI     STANDARD;      PRT;   533 AA.
AC   P12626;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1989, sequence version 1.
DT   21-MAR-2006, entry version 49.
DE   Nitrogen fixation protein anfA.
GN   Name=anfA;
OS   Azotobacter vinelandii.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Azotobacter.
OX   NCBI_TaxID=354;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=89255091; PubMed=2722750;
RA   Joerger R.D., Jacobson M.R., Bishop P.E.;
RT   "Two nifA-like genes required for expression of alternative
RT   nitrogenases by Azotobacter vinelandii.";
RL   J. Bacteriol. 171:3258-3267(1989).
CC   -!- FUNCTION: AnfA is essential for nitrogen fixation under Mo- and
CC       V-deficient conditions. It is required for the regulation of
CC       nitrogenase 3 transcription. Interacts with sigma-54.
CC   -!- SIMILARITY: Contains 1 GAF domain.
CC   -!- SIMILARITY: Contains 1 sigma-54 factor interaction domain.
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DR   EMBL; M26751; AAA22146.1; -; Genomic_DNA.
DR   PIR; A44514; A44514.
DR   InterPro; IPR003593; AAA_ATPase.
DR   InterPro; IPR008931; FIS-like.
DR   InterPro; IPR003018; GAF.
DR   InterPro; IPR002197; HTH_Fis.
DR   InterPro; IPR002078; Sig54_interact.
DR   Pfam; PF01590; GAF; 1.
DR   Pfam; PF02954; HTH_8; 1.
DR   Pfam; PF00158; Sigma54_activat; 1.
DR   PRINTS; PR01590; HTHFIS.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00065; GAF; 1.
DR   PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR   PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR   PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR   PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
KW   Activator; ATP-binding; DNA-binding; Nitrogen fixation;
KW   Nucleotide-binding; Sensory transduction; Transcription;
KW   Transcription regulation; Two-component regulatory system.
FT   CHAIN         1    533       Nitrogen fixation protein anfA.
FT                                /FTId=PRO_0000081316.
FT   DOMAIN       46    186       GAF.
FT   DOMAIN      219    448       Sigma-54 factor interaction.
FT   NP_BIND     247    254       ATP (Potential).
FT   NP_BIND     310    319       ATP (Potential).
FT   DNA_BIND    501    520       H-T-H motif.
FT   REGION       33    193       A domain.
SQ   SEQUENCE   533 AA;  59547 MW;  0DEFBE02A1CF7695 CRC64;
     MSDQATFEFD TDYFVEEFSH CFTGECRVKM LPILYKISQI ITGNADLADA LSIVLGVMQQ
     HLKMQRGIVT LYDMRAETIF IHDSFGLTEE EKKRGIYAVG EGITGKVVET GKAIVARRLQ
     EHPDFLGRTR VSRNGKAKAA FFCVPIMRAQ KVLGTIAAER VYMNPRLLKQ DVELLTMIAT
     MIAPLVELYL IENIERVRLE NENRRLKHAL KERFKPSNII GNSKPMQEVY ELIHKVASTK
     ATVLILGESG VGKELVANAI HYNSPNAEAA LVTSNCAPLP ENLAESELFG HEKGSFTGAL
     TMHKGCFEQA DGGTIFLDEV GELSPTVQAK LVRVLQNRTF ERVGGSKPVK VDVRIIAATN
     RNLVEMVEQG TFREDLYYRL NVFPITVPPL RERGSDVIAL ADHFVSAFSR ENGKNVKRIS
     TPALNMLMSY HWPGNVRELE NVMERAVILS DDDVIHSYNL PPSLQTSKES GTAFGLTLEE
     KIKAVECEMI VEALKNSSGH IGEAAKELGL ARRMLGVRME RYGISYKSFS RYA
//