ID   ANFD_AZOVI     STANDARD;      PRT;   517 AA.
AC   P16266;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   04-JAN-2005, sequence version 2.
DT   07-FEB-2006, entry version 48.
DE   Nitrogenase iron-iron protein alpha chain (EC 1.18.6.1) (Nitrogenase
DE   component I) (Dinitrogenase 3 alpha subunit).
GN   Name=anfD;
OS   Azotobacter vinelandii.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Azotobacter.
OX   NCBI_TaxID=354;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=89123105; PubMed=2644222;
RA   Joerger R.D., Jacobson M.R., Premakumar R., Wolfinger E.D.,
RA   Bishop P.E.;
RT   "Nucleotide sequence and mutational analysis of the structural genes
RT   (anfHDGK) for the second alternative nitrogenase from Azotobacter
RT   vinelandii.";
RL   J. Bacteriol. 171:1075-1086(1989).
RN   [2]
RP   PROTEIN SEQUENCE OF 1-6.
RC   STRAIN=RP306;
RX   MEDLINE=93319493; PubMed=8392330;
RA   Pau R.N., Eldridge M.E., Lowe D.J., Mitchenall L.A., Eady R.R.;
RT   "Molybdenum-independent nitrogenases of Azotobacter vinelandii: a
RT   functional species of alternative nitrogenase-3 isolated from a
RT   molybdenum-tolerant strain contains an iron-molybdenum cofactor.";
RL   Biochem. J. 293:101-107(1993).
CC   -!- FUNCTION: This iron-iron protein is part of the nitrogenase
CC       complex that catalyzes the key enzymatic reactions in nitrogen
CC       fixation. Other nitrogenase complexes utilize a molybdenum-iron
CC       protein or a vanadium-iron protein.
CC   -!- CATALYTIC ACTIVITY: 8 reduced ferredoxin + 8 H(+) + N(2) + 16 ATP
CC       + 16 H(2)O = 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 ADP + 16
CC       phosphate.
CC   -!- COFACTOR: Binds 1 8Fe-7S cluster per heterodimer (By similarity).
CC   -!- COFACTOR: Binds 1 8Fe-9S-X-homocitryl cluster per subunit (By
CC       similarity).
CC   -!- SUBUNIT: Hexamer of two alpha, two beta, and two delta chains.
CC   -!- SIMILARITY: Belongs to the nifD/nifK/nifE/nifN family.
CC   -!- CAUTION: The structure of the 8Fe-9S-X-homocitryl cluster is
CC       assumed to be analogous to the 7Fe-Mo-9S-X-homocitryl cluster. The
CC       identity of the X atom is not known, possibly carbon or oxygen.
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DR   EMBL; M23528; AAA82509.1; -; Genomic_DNA.
DR   PIR; B32057; B32057.
DR   HSSP; P00467; 1MIO.
DR   InterPro; IPR011290; ANFD.
DR   InterPro; IPR010143; N2-ase-Ialpha.
DR   InterPro; IPR005974; Nitrog_alpha.
DR   InterPro; IPR000318; Nitrognse_comp1.
DR   InterPro; IPR000510; Oxred_nitrognse1.
DR   Pfam; PF00148; Oxidored_nitro; 1.
DR   TIGRFAMs; TIGR01284; alt_nitrog_alph; 1.
DR   TIGRFAMs; TIGR01861; ANFD; 1.
DR   TIGRFAMs; TIGR01862; N2-ase-Ialpha; 1.
DR   PROSITE; PS00699; NITROGENASE_1_1; 1.
DR   PROSITE; PS00090; NITROGENASE_1_2; 1.
KW   Direct protein sequencing; Iron; Iron-sulfur; Metal-binding;
KW   Nitrogen fixation; Oxidoreductase.
FT   INIT_MET      0      0
FT   CHAIN         1    517       Nitrogenase iron-iron protein alpha
FT                                chain.
FT                                /FTId=PRO_0000153059.
FT   METAL        48     48       Iron-sulfur (8Fe-7S) (shared with beta
FT                                chain) (By similarity).
FT   METAL        74     74       Iron-sulfur (8Fe-7S) (shared with beta
FT                                chain) (By similarity).
FT   METAL       137    137       Iron-sulfur (8Fe-7S) (shared with beta
FT                                chain) (By similarity).
FT   METAL       256    256       Iron-sulfur (8Fe-9S-X-homocitryl) (By
FT                                similarity).
FT   METAL       422    422       Iron-sulfur (8Fe-9S-X-homocitryl) (via
FT                                pros nitrogen) (By similarity).
FT   CONFLICT      3      3       H -> E (in Ref. 2).
SQ   SEQUENCE   517 AA;  58282 MW;  D84B513BD2E66F8B CRC64;
     PHHEFECSKV IPERKKHAVI KGKGETLADA LPQGYLNTIP GSISERGCAY CGAKHVIGTP
     MKDVIHISHG PVGCTYDTWQ TKRYISDNDN FQLKYTYATD VKEKHIVFGA EKLLKQNIIE
     AFKAFPQIKR MTIYQTCATA LIGDDINAIA EEVMEEMPEV DIFVCNSPGF AGPSQSGGHH
     KINIAWINQK VGTVEPEITG DHVINYVGEY NIQGDQEVMV DYFKRMGIQV LSTFTGNGSY
     DGLRAMHRAH LNVLECARSA EYICNELRVR YGIPRLDIDG FGFKPLADSL RKIGMFFGIE
     DRAKAIIDEE VARWKPELDW YKERLMGKKV CLWPGGSKLW HWAHVIEEEM GLKVVSVYIK
     FGHQGDMEKG IARCGEGTLA IDDPNELEGL EALEMLKPDI ILTGKRPGEV AKKVRVPYLN
     AHAYHNGPYK GFEGWVRFAR DIYNAIYSPI HQLSGIDITK DNAPEWGNGF RTRQMLSDGN
     LSDAVRNSET LRQYTGGYDS VSKLREREYP AFERKVG
//