ID ANFG_AZOVI STANDARD; PRT; 131 AA. AC P16268; DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot. DT 04-JAN-2005, sequence version 2. DT 07-FEB-2006, entry version 36. DE Nitrogenase iron-iron protein delta chain (EC 1.18.6.1) (Nitrogenase DE component I) (Dinitrogenase 3 delta subunit). GN Name=anfG; OS Azotobacter vinelandii. OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Azotobacter. OX NCBI_TaxID=354; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX MEDLINE=89123105; PubMed=2644222; RA Joerger R.D., Jacobson M.R., Premakumar R., Wolfinger E.D., RA Bishop P.E.; RT "Nucleotide sequence and mutational analysis of the structural genes RT (anfHDGK) for the second alternative nitrogenase from Azotobacter RT vinelandii."; RL J. Bacteriol. 171:1075-1086(1989). RN [2] RP PROTEIN SEQUENCE OF 1-7. RC STRAIN=RP306; RX MEDLINE=93319493; PubMed=8392330; RA Pau R.N., Eldridge M.E., Lowe D.J., Mitchenall L.A., Eady R.R.; RT "Molybdenum-independent nitrogenases of Azotobacter vinelandii: a RT functional species of alternative nitrogenase-3 isolated from a RT molybdenum-tolerant strain contains an iron-molybdenum cofactor."; RL Biochem. J. 293:101-107(1993). CC -!- FUNCTION: The key enzymatic reactions in nitrogen fixation are CC catalyzed by the nitrogenase complex, which has 2 components: the CC iron protein (component 2) and a component 1 which is either a CC molybdenum-iron protein, a vanadium-iron, or an iron-iron protein. CC -!- CATALYTIC ACTIVITY: 8 reduced ferredoxin + 8 H(+) + N(2) + 16 ATP CC + 16 H(2)O = 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 ADP + 16 CC phosphate. CC -!- COFACTOR: Iron-sulfur. CC -!- SUBUNIT: Hexamer of two alpha, two beta, and two delta chains. CC ----------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution-NoDerivs License CC ----------------------------------------------------------------------- DR EMBL; M23528; AAA82510.1; -; Genomic_DNA. DR PIR; C32057; C32057. DR InterPro; IPR004349; AnfG_VnfG. DR Pfam; PF03139; AnfG_VnfG; 1. DR ProDom; PD006096; AnfG_VnfG; 1. KW Direct protein sequencing; Iron; Iron-sulfur; Metal-binding; KW Nitrogen fixation; Oxidoreductase. FT INIT_MET 0 0 FT CHAIN 1 131 Nitrogenase iron-iron protein delta FT chain. FT /FTId=PRO_0000213563. SQ SEQUENCE 131 AA; 15212 MW; A19DB8AD01531A35 CRC64; STASAAAVVK QKVEAPVHPM DARIDELTDY IMKNCLWQFH SRSWDRERQN AEILKKTKEL LCGEPVDLST SHDRCYWVDA VCLADDYREH YPWINSMSKE EIGSLMQGLK DRMDYLTITG SLNEELSDKH Y //