ID   BA72_EUBSP     STANDARD;      PRT;   249 AA.
AC   P19337;
DT   01-NOV-1990 (Rel. 16, Created)
DT   01-NOV-1990 (Rel. 16, Last sequence update)
DT   25-OCT-2004 (Rel. 45, Last annotation update)
DE   7-alpha-hydroxysteroid dehydrogenase (EC 1.1.1.159) (Bile acid 7-
DE   dehydroxylase) (7-alpha-HSDH) (Bile acid-inducible protein).
GN   Name=baiA2;
OS   Eubacterium sp. (strain VPI 12708).
OC   Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=29347;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   MEDLINE=91072253; PubMed=2254270;
RA   Mallonee D.H., White W.B., Hylemon P.B.;
RT   "Cloning and sequencing of a bile acid-inducible operon from
RT   Eubacterium sp. strain VPI 12708.";
RL   J. Bacteriol. 172:7011-7019(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RX   MEDLINE=90330548; PubMed=2376563;
RA   Gopal-Srivastava R., Mallonee D.H., White W.B., Hylemon P.B.;
RT   "Multiple copies of a bile acid-inducible gene in Eubacterium sp.
RT   strain VPI 12708.";
RL   J. Bacteriol. 172:4420-4426(1990).
RN   [3]
RP   NUCLEOTIDE SEQUENCE.
RX   MEDLINE=90264339; PubMed=2345152;
RA   Mallonee D.H., White W.B., Hylemon P.B.;
RT   "Nucleotide sequences and mutational analysis of the structural genes
RT   for nitrogenase 2 of Azotobacter vinelandii.";
RL   J. Bacteriol. 172:3400-3408(1990).
RN   [4]
RP   NUCLEOTIDE SEQUENCE.
RX   MEDLINE=89008068; PubMed=3170477;
RA   White W.B., Franklund C.V., Coleman J.P., Hylemon P.B.;
RT   "Evidence for a multigene family involved in bile acid 7-
RT   dehydroxylation in Eubacterium sp. strain VPI 12708.";
RL   J. Bacteriol. 170:4555-4561(1988).
CC   -!- FUNCTION: 7-alpha-dehydroxylation of cholic acid, yielding
CC       deoxycholic acid and lithocholic acid, respectively. Highest
CC       affinity with taurochenodeoxycholic acid.
CC   -!- CATALYTIC ACTIVITY: 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-
CC       cholanate + NAD(+) = 3-alpha,12-alpha-dihydroxy-7-oxo-5-beta-
CC       cholanate + NADH.
CC   -!- PATHWAY: Bile acid catabolism.
CC   -!- INDUCTION: Presence of C(24) bile acids containing a 7-alpha-
CC       hydroxy group.
CC   -!- MISCELLANEOUS: There are three genes for BAIA proteins: BAIA1 is
CC       identical to BAIA3 and there is 81% identity with BAIA2.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases
CC       (SDR) family.
CC   --------------------------------------------------------------------------
CC   This Swiss-Prot entry is copyright. It is produced through a collaboration
CC   between  the Swiss Institute of Bioinformatics  and the  EMBL outstation -
CC   the European Bioinformatics Institute.  There are no  restrictions on  its
CC   use as long as its content is in no way modified and this statement is not
CC   removed.
CC   --------------------------------------------------------------------------
DR   EMBL; M22623; AAB61150.1; -.
DR   EMBL; U57489; AAC45414.1; -.
DR   PIR; A31841; A31841.
DR   HSSP; Q01782; 1E7W.
DR   InterPro; IPR002198; ADH_short.
DR   InterPro; IPR002347; Adh_short_C2.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   PROSITE; PS00061; ADH_SHORT; 1.
KW   Bile acid catabolism; Multigene family; NAD; Oxidoreductase.
FT   NP_BIND       7     13       NAD (Potential).
FT   NP_BIND      32     36       NAD (Potential).
FT   ACT_SITE    157    157       Proton acceptor (By similarity).
FT   BINDING     144    144       Substrate (By similarity).
SQ   SEQUENCE   249 AA;  26538 MW;  9A39B78BB63DC5AF CRC64;
     MNLVQDKVTI ITGGTRGIGF AAAKIFIDNG AKVSIFGETQ EEVDTALAQL KELYPEEEVL
     GFAPDLTSRD AVMAAVGQVA QKYGRLDVMI NNAGITSNNV FSRVSEEEFK HIMDINVTGV
     FNGAWCAYQC MKDAKKGVII NTASVTGIFG SLSGVGYPAS KASVIGLTHG LGREIIRKNI
     RVVGVAPGVV NTDMTNGNPP EIMEGYLKAL PMKRMLEPEE IANVYLFLAS DLASGITATT
     VSVDGAYRP
//