ID   BAIA2_EUBSP    STANDARD;      PRT;   249 AA.
AC   P19337;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   07-MAR-2006, entry version 46.
DE   Bile acid 7-dehydroxylase 2 (EC 1.17.1.6) (Cholate 7-alpha-
DE   dehydroxylase 2) (Bile acid-inducible protein 2).
GN   Name=baiA2;
OS   Eubacterium sp. (strain VPI 12708).
OC   Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=29347;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=91072253; PubMed=2254270;
RA   Mallonee D.H., White W.B., Hylemon P.B.;
RT   "Cloning and sequencing of a bile acid-inducible operon from
RT   Eubacterium sp. strain VPI 12708.";
RL   J. Bacteriol. 172:7011-7019(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=90330548; PubMed=2376563;
RA   Gopal-Srivastava R., Mallonee D.H., White W.B., Hylemon P.B.;
RT   "Multiple copies of a bile acid-inducible gene in Eubacterium sp.
RT   strain VPI 12708.";
RL   J. Bacteriol. 172:4420-4426(1990).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=89008068; PubMed=3170477;
RA   White W.B., Franklund C.V., Coleman J.P., Hylemon P.B.;
RT   "Evidence for a multigene family involved in bile acid
RT   7-dehydroxylation in Eubacterium sp. strain VPI 12708.";
RL   J. Bacteriol. 170:4555-4561(1988).
CC   -!- FUNCTION: 7-alpha-dehydroxylation of cholic acid and
CC       chenodeoxycholate, yielding deoxycholic acid and lithocholic acid,
CC       respectively. Highest affinity with taurochenodeoxycholic acid.
CC   -!- CATALYTIC ACTIVITY: Deoxycholate + NAD(+) + H(2)O = cholate +
CC       NADH.
CC   -!- CATALYTIC ACTIVITY: Lithocholate + NAD(+) + H(2)O =
CC       chenodeoxycholate + NADH.
CC   -!- PATHWAY: Bile acid catabolism.
CC   -!- INDUCTION: Presence of C(24) bile acids containing a 7-alpha-
CC       hydroxy group.
CC   -!- MISCELLANEOUS: There are three genes for baiA proteins: baiA1 is
CC       identical to baiA3 and there is 81% identity with baiA2.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases
CC       (SDR) family.
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DR   EMBL; M22623; AAB61150.1; -; Genomic_DNA.
DR   EMBL; U57489; AAC45414.1; -; Genomic_DNA.
DR   PIR; A31841; A31841.
DR   HSSP; Q01782; 1E7W.
DR   InterPro; IPR002347; ADH_short_C2.
DR   InterPro; IPR002198; SDR.
DR   PANTHER; PTHR19410; ADH_short; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   PROSITE; PS00061; ADH_SHORT; 1.
KW   Bile acid catabolism; Lipid metabolism; NAD; Oxidoreductase;
KW   Steroid metabolism.
FT   CHAIN         1    249       Bile acid 7-dehydroxylase 2.
FT                                /FTId=PRO_0000054525.
FT   NP_BIND       7     13       NAD (Potential).
FT   NP_BIND      32     36       NAD (Potential).
FT   ACT_SITE    157    157       Proton acceptor (By similarity).
FT   BINDING     144    144       Substrate (By similarity).
SQ   SEQUENCE   249 AA;  26538 MW;  9A39B78BB63DC5AF CRC64;
     MNLVQDKVTI ITGGTRGIGF AAAKIFIDNG AKVSIFGETQ EEVDTALAQL KELYPEEEVL
     GFAPDLTSRD AVMAAVGQVA QKYGRLDVMI NNAGITSNNV FSRVSEEEFK HIMDINVTGV
     FNGAWCAYQC MKDAKKGVII NTASVTGIFG SLSGVGYPAS KASVIGLTHG LGREIIRKNI
     RVVGVAPGVV NTDMTNGNPP EIMEGYLKAL PMKRMLEPEE IANVYLFLAS DLASGITATT
     VSVDGAYRP
//