ID   BOFC_BACSU     STANDARD;      PRT;   170 AA.
AC   O05391;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   07-MAR-2006, entry version 37.
DE   Protein bofC precursor (Bypass-of-forespore protein C).
GN   Name=bofC; OrderedLocusNames=BSU27750;
OS   Bacillus subtilis.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1423;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / PY79;
RX   MEDLINE=97177783; PubMed=9025289;
RA   Gomez M., Cutting S.M.;
RT   "BofC encodes a putative forespore regulator of the Bacillus subtilis
RT   sigma K checkpoint.";
RL   Microbiology 143:157-170(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RA   Tosato V., Bolotin A., Bertani I., Valentino I., Bruschi C.V.;
RT   "A 17.8 kb segment in the spoVB-nadC region of the Bacillus subtilis
RT   168 chromosome: sequencing and ruv operon identification.";
RL   Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   MEDLINE=98044033; PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA   Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA   Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA   Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA   Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA   Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA   Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA   Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA   Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA   Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA   Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA   Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA   Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA   Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA   Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA   Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA   Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA   Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA   Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA   Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA   Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA   Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   STRUCTURE BY NMR OF 31-170, AND SUBUNIT.
RX   PubMed=16049010; DOI=10.1074/jbc.M506910200;
RA   Patterson H.M., Brannigan J.A., Cutting S.M., Wilson K.S.,
RA   Wilkinson A.J., Ab E., Diercks T., de Jong R.N., Truffault V.,
RA   Folkers G.E., Kaptein R.;
RT   "The structure of bypass of forespore C, an intercompartmental
RT   signaling factor during sporulation in Bacillus.";
RL   J. Biol. Chem. 280:36214-36220(2005).
CC   -!- FUNCTION: Inhibits the spoIVB zymogen from undergoing
CC       autocatalytic activation by an unknown mechanism, and in this way
CC       plays a role in the sigma-K checkpoint of sporulation.
CC   -!- SUBUNIT: Monomer.
CC   -!- SUBCELLULAR LOCATION: Forespore intermembrane space.
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DR   EMBL; X93081; CAA63621.1; -; Genomic_DNA.
DR   EMBL; Y15896; CAB75329.1; -; Genomic_DNA.
DR   EMBL; Z99118; CAB14735.1; -; Genomic_DNA.
DR   PIR; A69596; A69596.
DR   PDB; 2BW2; NMR; A=31-170.
DR   SubtiList; BG11917; bofC.
DR   GenomeReviews; AL009126_GR; BSU27750.
DR   BioCyc; BSUB1423:BSU2771-MONOMER; -.
KW   3D-structure; Complete proteome; Signal; Sporulation.
FT   SIGNAL        1     30       Probable.
FT   CHAIN        31    170       Protein bofC.
FT                                /FTId=PRO_0000020824.
FT   VARIANT      96     96       S -> F (in bofC1).
FT   STRAND       34     48
FT   STRAND       50     52
FT   STRAND       54     65
FT   HELIX        66     72
FT   STRAND       73     73
FT   TURN         74     75
FT   STRAND       76     82
FT   TURN         83     84
FT   STRAND       85     91
FT   STRAND       93     94
FT   TURN         97     97
FT   HELIX        98    100
FT   TURN        101    101
FT   STRAND      105    108
FT   TURN        109    111
FT   STRAND      112    119
FT   STRAND      121    121
FT   TURN        122    123
FT   STRAND      124    124
FT   STRAND      126    127
FT   TURN        135    137
FT   STRAND      138    138
FT   HELIX       140    148
FT   STRAND      151    151
FT   HELIX       155    168
SQ   SEQUENCE   170 AA;  19296 MW;  2786FEE1DBDFD2A2 CRC64;
     MKRFSTAYLL LGILCSAAVF LIGAPSRALG AEVEHYEPLQ VHVQLEKVYL DGDVSIEHKH
     EKVFSMDDFW AAYAGWTLVE QKKGYVLFRK QMDDISPLSK VNGYIGVSDN GVISTFHGRP
     EPASEPIQSF FQIDLERLES HMQKNLLKGI PFRTKAEFED VIEHMKTYSG
//