ID   ALN_BACSU      STANDARD;      PRT;   446 AA.
AC   O32137;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   21-MAR-2006, entry version 45.
DE   Allantoinase (EC 3.5.2.5).
GN   Name=pucH; OrderedLocusNames=BSU32410;
OS   Bacillus subtilis.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1423;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   MEDLINE=98044033; PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA   Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA   Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA   Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA   Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA   Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA   Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA   Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA   Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA   Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA   Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA   Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA   Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA   Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA   Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA   Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA   Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA   Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA   Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA   Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA   Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA   Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [2]
RP   FUNCTION.
RC   STRAIN=168;
RX   MEDLINE=21242727; PubMed=11344136;
RX   DOI=10.1128/JB.183.11.3293-3302.2001;
RA   Schultz A.C., Nygaard P., Saxild H.H.;
RT   "Functional analysis of 14 genes that constitute the purine catabolic
RT   pathway in Bacillus subtilis and evidence for a novel regulon
RT   controlled by the PucR transcription activator.";
RL   J. Bacteriol. 183:3293-3302(2001).
CC   -!- CATALYTIC ACTIVITY: (S)-allantoin + H(2)O = allantoate.
CC   -!- COFACTOR: Zinc (By similarity).
CC   -!- PATHWAY: Degradation of allantoin (purine catabolism); first step.
CC   -!- INDUCTION: Expression is very low in excess nitrogen (glutamate
CC       plus ammonia) and is induced during limiting-nitrogen conditions
CC       (glutamate). Expression is further induced when allantoin or
CC       allantoate are added during limiting-nitrogen conditions.
CC   -!- SIMILARITY: Belongs to the DHOase family.
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DR   EMBL; Z99120; CAB15231.1; -; Genomic_DNA.
DR   PIR; C70016; C70016.
DR   HSSP; P81006; 1GKR.
DR   SubtiList; BG13982; pucH.
DR   GenomeReviews; AL009126_GR; BSU32410.
DR   BioCyc; BSUB1423:BSU3238-MONOMER; -.
DR   InterPro; IPR006680; Amidohydro_1.
DR   InterPro; IPR011059; Metalo_hydrolase.
DR   Pfam; PF01979; Amidohydro_1; 1.
KW   Complete proteome; Hydrolase; Metal-binding; Purine metabolism; Zinc.
FT   CHAIN         1    446       Allantoinase.
FT                                /FTId=PRO_0000165940.
FT   METAL        60     60       Zinc (Potential).
FT   METAL        62     62       Zinc (Potential).
SQ   SEQUENCE   446 AA;  48331 MW;  48515B6414A5C234 CRC64;
     MAYDMVIKGA KAVTPDGVIE ADIVVQNGVI AEIGSDIEAS GTEIIQADGK YVFPGVIDCH
     VHFNEPGRED WEGFETGSQM MAAGGCTTYF DMPLNCIPST VTAEHLLAKA ELGRQKSAVD
     FALWGGLVPG HIEDIRPMAE AGAIGFKAFL SKSGTDEFRS VDERTLLKGM AEIAAAGKIL
     ALHAESDAIT SYLQMVLANK GKVDADAYAA SRPEEAEVEA VYRTIQYAKV TGCPVHFVHI
     STAKAVRLIR EAKQEGLDVS VETCPHYVLF SHDDLRQRGS VAKCAPPLRS RQSKETLIET
     LIAGDIDMVS SDHSPCRPSL KREDNMFLSW GGISGGQFTL LGMLELALEH QIPFETIAEW
     TAAAPAKRFG LQKKGRLEAG CDADFVLVSM EPYTVTRESM FAKHKKSIYE GHTFPCSISA
     TYSKGRCVYN DGEKVTEIDG ALVVPS
//