ID   BMR1_BACSU     STANDARD;      PRT;   389 AA.
AC   P33449;
DT   01-FEB-1994 (Rel. 28, Created)
DT   01-FEB-1994 (Rel. 28, Last sequence update)
DT   01-FEB-2005 (Rel. 46, Last annotation update)
DE   Multidrug resistance protein 1 (Multidrug-efflux transporter 1).
GN   Name=bmr; Synonyms=bmr1; OrderedLocusNames=BSU24010;
OS   Bacillus subtilis.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1423;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=168 / BD170;
RX   MEDLINE=91271277; PubMed=1675788;
RA   Neyfakh A.A., Bidnenko V.E., Chen L.B.;
RT   "Efflux-mediated multidrug resistance in Bacillus subtilis:
RT   similarities and dissimilarities with the mammalian system.";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:4781-4785(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=168 / Marburg;
RX   MEDLINE=95050642; PubMed=7961792;
RA   Ahmed M., Borsch C.M., Taylor S.S., Vazquez-Laslop N., Neyfakh A.A.;
RT   "A protein that activates expression of a multidrug efflux transporter
RT   upon binding the transporter substrates.";
RL   J. Biol. Chem. 269:28506-28513(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=168 / JH642;
RX   MEDLINE=97124195; PubMed=8969508;
RA   Mizuno M., Masuda S., Takemaru K.-I., Hosono S., Sato T., Takeuchi M.,
RA   Kobayashi Y.;
RT   "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of
RT   the Bacillus subtilis genome containing the skin element and many
RT   sporulation genes.";
RL   Microbiology 142:3103-3111(1996).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   MEDLINE=98044033; PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA   Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA   Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA   Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA   Choi S.K., Codani J.J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA   Denizot F., Devine K.M., Dusterhoft A., Ehrlich S.D., Emmerson P.T.,
RA   Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA   Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA   Ghim S.Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA   Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA   Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA   Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA   Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA   Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA   Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA   Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA   Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA   Shin B.S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA   Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA   Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA   Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA   Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
CC   -!- FUNCTION: Energy-dependent efflux pump responsible for decreased
CC       drug accumulation in multi-drug-resistant cells. Probably uses a
CC       transmembrane proton gradient as the energy source. Causes the
CC       efflux of a variety of toxic substances, including such
CC       structurally diverse compounds as ethidium bromide, rhodamine and
CC       acridine dyes, tetraphenylphosphonium, puromycin, chloramphenicol,
CC       doxorubicin, and fluoroquinolone antibiotics.
CC   -!- SUBCELLULAR LOCATION: Integral membrane protein.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. TCR/tetA
CC       family.
CC   --------------------------------------------------------------------------
CC   This Swiss-Prot entry is copyright. It is produced through a collaboration
CC   between  the Swiss Institute of Bioinformatics  and the  EMBL outstation -
CC   the European Bioinformatics Institute.  There are no  restrictions on  its
CC   use as long as its content is in no way modified and this statement is not
CC   removed.
CC   --------------------------------------------------------------------------
DR   EMBL; M33768; AAA22277.1; -.
DR   EMBL; L25604; AAB81539.1; -.
DR   EMBL; D84432; BAA12601.1; -.
DR   EMBL; Z99116; CAB14332.1; -.
DR   PIR; A39705; A39705.
DR   SubtiList; BG10303; bmr.
DR   InterPro; IPR007114; MFS.
DR   InterPro; IPR005828; Sub_transporter.
DR   InterPro; IPR005829; Sug_transporter.
DR   InterPro; IPR001958; TCR_TetA.
DR   Pfam; PF00083; Sugar_tr; 1.
DR   PRINTS; PR01035; TCRTETA.
DR   PROSITE; PS50850; MFS; 1.
DR   PROSITE; PS00216; SUGAR_TRANSPORT_1; UNKNOWN_1.
KW   Antibiotic resistance; Complete proteome; Transmembrane; Transport.
FT   TRANSMEM      6     26       Potential.
FT   TRANSMEM     42     62       Potential.
FT   TRANSMEM     71     91       Potential.
FT   TRANSMEM    102    122       Potential.
FT   TRANSMEM    134    154       Potential.
FT   TRANSMEM    160    180       Potential.
FT   TRANSMEM    202    222       Potential.
FT   TRANSMEM    243    263       Potential.
FT   TRANSMEM    286    306       Potential.
FT   TRANSMEM    336    356       Potential.
FT   TRANSMEM    358    378       Potential.
SQ   SEQUENCE   389 AA;  42258 MW;  3A570DEE5836A127 CRC64;
     MEKKNITLTI LLTNLFIAFL GIGLVIPVTP TIMNELHLSG TAVGYMVACF AITQLIVSPI
     AGRWVDRFGR KIMIVIGLLF FSVSEFLFGI GKTVEMLFIS RMLGGISAPF IMPGVTAFIA
     DITTIKTRPK ALGYMSAAIS TGFIIGPGIG GFLAEVHSRL PFFFAAAFAL LAAILSILTL
     REPERNPENQ EIKGQKTGFK RIFAPMYFIA FLIILISSFG LASFESLFAL FVDHKFGFTA
     SDIAIMITGG AIVGAITQVV LFDRFTRWFG EIHLIRYSLI LSTSLVFLLT TVHSYVAILL
     VTVTVFVGFD LMRPAVTTYL SKIAGNEQGF AGGMNSMFTS IGNVFGPIIG GMLFDIDVNY
     PFYFATVTLA IGIALTIAWK APAHLKAST
//