ID   AMAA_BACST     STANDARD;      PRT;   370 AA.
AC   P37112;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   02-MAY-2006, entry version 41.
DE   N-acyl-L-amino acid amidohydrolase (EC 3.5.1.14) (L-aminoacylase).
GN   Name=amaA; Synonyms=ama;
OS   Bacillus stearothermophilus (Geobacillus stearothermophilus).
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Geobacillus.
OX   NCBI_TaxID=1422;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10, AND
RP   CHARACTERIZATION.
RC   STRAIN=NCIB 8224;
RX   MEDLINE=94113715; PubMed=8285691;
RA   Sakanyan V., Desmarez L., Legrain C., Charlier D.R.M., Mett I.,
RA   Kochikyan A., Savchenko A., Boyen A., Falmagne P., Pirard A.,
RA   Glansdorff N.;
RT   "Gene cloning, sequence analysis, purification, and characterization
RT   of a thermostable aminoacylase from Bacillus stearothermophilus.";
RL   Appl. Environ. Microbiol. 59:3878-3888(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NCIB 8224;
RX   MEDLINE=97176409; PubMed=9023955;
RA   Batisse N., Weigel P., Lecocq M., Sakanyan V.;
RT   "Two amino acid amidohydrolase genes encoding L-stereospecific
RT   carbamoylase and aminoacylase are organized in a common operon in
RT   Bacillus stearothermophilus.";
RL   Appl. Environ. Microbiol. 63:763-766(1997).
CC   -!- FUNCTION: Hydrolyzes most efficiently N-acetyl derivatives of
CC       aromatic amino acids but is also active on other amino acids.
CC       L-stereospecific.
CC   -!- CATALYTIC ACTIVITY: An N-acyl-L-amino acid + H(2)O = a carboxylate
CC       + an L-amino acid.
CC   -!- COFACTOR: Cobalt.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SIMILARITY: Belongs to the peptidase M20 family.
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DR   EMBL; X74289; CAA52342.1; -; Genomic_DNA.
DR   EMBL; Y08753; CAA70000.1; -; Genomic_DNA.
DR   PIR; I40358; I40358.
DR   LinkHub; P37112; -.
DR   InterPro; IPR011650; M20_dimer.
DR   InterPro; IPR010168; Pept_M20D_amidh.
DR   InterPro; IPR002933; Peptidase_M20.
DR   Pfam; PF07687; M20_dimer; 1.
DR   Pfam; PF01546; Peptidase_M20; 1.
DR   TIGRFAMs; TIGR01891; amidohydrolases; 1.
KW   Cobalt; Direct protein sequencing; Hydrolase.
FT   CHAIN         1    370       N-acyl-L-amino acid amidohydrolase.
FT                                /FTId=PRO_0000061949.
SQ   SEQUENCE   370 AA;  41676 MW;  479AF1559DB527FB CRC64;
     MTKEEIKRLV DEVKTDVIAW RRHLHAHPEL SFQEEKTAQF VYETLQSFGH LELSRPTKTS
     VMARLIGQQP GRVVAIRADM DALPIQEENT FEFASKNPGV MHACGHDGHT AMLLGTAKIF
     SQLRDDIRGE IRFLFQHAEE LFPGGAEEMV QAGVMDGVDV VIGTHLWSPL ERGKIGIVYG
     PMMAAPDRFF IRIIGKGGHG AMPHQTIDAI AIGAQVVTNL QHIVSRYVDP LEPLVLSVTQ
     FVAGTAHNVL PGEVEIQGTV RTFDETLRRT VPQWMERIVK GITEAHGASY EFRFDYGYRP
     VINYDEGDPR HGGNGVRAVR RRGSGPLETE HGRRRFLRLF AKSARQLFLR RRGQCRKRHR
     LPAPPPALYD
//