ID   AMAB1_BACST    STANDARD;      PRT;   409 AA.
AC   P37113; P94345;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   02-MAY-2006, entry version 31.
DE   N-carbamoyl-L-amino acid hydrolase (EC 3.5.1.87) (L-carbamoylase).
GN   Name=amaB;
OS   Bacillus stearothermophilus (Geobacillus stearothermophilus).
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Geobacillus.
OX   NCBI_TaxID=1422;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NCIB 8224;
RX   MEDLINE=97176409; PubMed=9023955;
RA   Batisse N., Weigel P., Lecocq M., Sakanyan V.;
RT   "Two amino acid amidohydrolase genes encoding L-stereospecific
RT   carbamoylase and aminoacylase are organized in a common operon in
RT   Bacillus stearothermophilus.";
RL   Appl. Environ. Microbiol. 63:763-766(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 60-409.
RC   STRAIN=NCIB 8224;
RX   MEDLINE=94113715; PubMed=8285691;
RA   Sakanyan V., Desmarez L., Legrain C., Charlier D.R.M., Mett I.,
RA   Kochikyan A., Savchenko A., Boyen A., Falmagne P., Pirard A.,
RA   Glansdorff N.;
RT   "Gene cloning, sequence analysis, purification, and characterization
RT   of a thermostable aminoacylase from Bacillus stearothermophilus.";
RL   Appl. Environ. Microbiol. 59:3878-3888(1993).
CC   -!- CATALYTIC ACTIVITY: N-carbamoyl-L-2-amino acid (a 2-ureido
CC       carboxylate) + H(2)O = L-2-amino acid + NH(3) + CO(2).
CC   -!- COFACTOR: Manganese or nickel or cobalt.
CC   -!- SIMILARITY: Belongs to the peptidase M20 family.
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DR   EMBL; Y08752; CAA69999.1; -; Genomic_DNA.
DR   EMBL; X74289; CAA52341.1; -; Genomic_DNA.
DR   InterPro; IPR010158; Hydantase.
DR   InterPro; IPR011650; M20_dimer.
DR   InterPro; IPR002933; Peptidase_M20.
DR   Pfam; PF07687; M20_dimer; 1.
DR   Pfam; PF01546; Peptidase_M20; 1.
DR   TIGRFAMs; TIGR01879; hydantase; 1.
KW   Hydrolase.
FT   CHAIN         1    409       N-carbamoyl-L-amino acid hydrolase.
FT                                /FTId=PRO_0000061952.
FT   CONFLICT     60     62       LIG -> DRE (in Ref. 2).
SQ   SEQUENCE   409 AA;  44167 MW;  9A91F767C9AEFBBB CRC64;
     MIQGERLWQR LMELGEVGKQ PSGGVTRLSF TAEERRAKDL VASYMREAGL FVYEDAAGNL
     IGRKEGTNPD ATVVLVGSHL DSVYNGGCFD GPLGVLAGVE VVQTMNEHGV VTHHPIEVVA
     FTDEEGARFR FGMIGSRAMA GTLPPEALEC RDAEGISLAE AMKQAGLDPD RLPQAARKPG
     TVKAYVELHI EQGRVLEEAG LPVGIVTGIA GLIWVKFTIA GPAEHAGATP MSLRRDPMAA
     AAQIIIVIEE EARRTGTTVG TVGQLHVYPG GINVIPERVE FVLDLRDLKA EVRDQVWKAI
     AVRAETIAKE RNVRLTTERL QEMAPVLCSE VVKQAAERAC KQLGYPPFWL PSGAAHDGVQ
     LAPICPIGMI FVRSQDGVSH SPAEWSTKED CAVGAEVLYH TVWQLAQGE
//