ID BMRR_BACSU STANDARD; PRT; 278 AA. AC P39075; DT 01-FEB-1995 (Rel. 31, Created) DT 29-MAR-2004 (Rel. 43, Last sequence update) DT 01-FEB-2005 (Rel. 46, Last annotation update) DE Multidrug-efflux transporter 1 regulator. GN Name=bmrR; Synonyms=bmr1R; OrderedLocusNames=BSU24020; OS Bacillus subtilis. OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. OX NCBI_TaxID=1423; RN [1] RP NUCLEOTIDE SEQUENCE. RC STRAIN=168 / Marburg; RX MEDLINE=95050642; PubMed=7961792; RA Ahmed M., Borsch C.M., Taylor S.S., Vazquez-Laslop N., Neyfakh A.A.; RT "A protein that activates expression of a multidrug efflux transporter RT upon binding the transporter substrates."; RL J. Biol. Chem. 269:28506-28513(1994). RN [2] RP SEQUENCE REVISION. RA Neyfakh A.A.; RL Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases. RN [3] RP NUCLEOTIDE SEQUENCE. RC STRAIN=168 / JH642; RX MEDLINE=97124195; PubMed=8969508; RA Mizuno M., Masuda S., Takemaru K.-I., Hosono S., Sato T., Takeuchi M., RA Kobayashi Y.; RT "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of RT the Bacillus subtilis genome containing the skin element and many RT sporulation genes."; RL Microbiology 142:3103-3111(1996). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=168; RX MEDLINE=98044033; PubMed=9384377; DOI=10.1038/36786; RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., RA Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., RA Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., RA Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M., RA Choi S.K., Codani J.J., Connerton I.F., Cummings N.J., Daniel R.A., RA Denizot F., Devine K.M., Dusterhoft A., Ehrlich S.D., Emmerson P.T., RA Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., RA Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., RA Ghim S.Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., RA Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., RA Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., RA Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., RA Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., RA Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., RA Lazarevic V., Lee S.M., Levine A., Liu H., Masuda S., Mauel C., RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., RA Park S.H., Parro V., Pohl T.M., Portetelle D., Porwollik S., RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., RA Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., RA Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., RA Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., RA Shin B.S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., RA Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., RA Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., RA Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., RA Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.F., Zumstein E., RA Yoshikawa H., Danchin A.; RT "The complete genome sequence of the Gram-positive bacterium Bacillus RT subtilis."; RL Nature 390:249-256(1997). RN [5] RP SEQUENCE REVISION. RX MEDLINE=20036940; PubMed=10568751; DOI=10.1101/gr.9.11.1116; RA Medigue C., Rose M., Viari A., Danchin A.; RT "Detecting and analyzing DNA sequencing errors: toward a higher RT quality of the Bacillus subtilis genome sequence."; RL Genome Res. 9:1116-1127(1999). RN [6] RP X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 120-228. RX MEDLINE=99148268; PubMed=10025401; DOI=10.1016/S0092-8674(00)80548-6; RA Zheleznova E.E., Markham P.N., Neyfakh A.A., Brennan R.G.; RT "Structural basis of multidrug recognition by BmrR, a transcription RT activator of a multidrug transporter."; RL Cell 96:353-362(1999). RN [7] RP X-RAY CRYSTALLOGRAPHY (3.12 ANGSTROMS). RX MEDLINE=21069064; PubMed=11201751; DOI=10.1038/35053138; RA Heldwein E.E., Brennan R.G.; RT "Crystal structure of the transcription activator BmrR bound to DNA RT and a drug."; RL Nature 409:378-382(2001). CC -!- FUNCTION: Activates transcription of the bmr gene in response to CC structurally dissimilar drugs. Binds rhodamine as an inducer. CC -!- SUBUNIT: Binds DNA as a homodimer. CC -!- SIMILARITY: Contains 1 HTH merR-type DNA-binding domain. CC -!- CAUTION: Ref.3 sequence differs from that shown due to frameshifts CC in positions 74 and 120. CC -------------------------------------------------------------------------- CC This Swiss-Prot entry is copyright. It is produced through a collaboration CC between the Swiss Institute of Bioinformatics and the EMBL outstation - CC the European Bioinformatics Institute. There are no restrictions on its CC use as long as its content is in no way modified and this statement is not CC removed. CC -------------------------------------------------------------------------- DR EMBL; L25604; AAB81540.2; -. DR EMBL; D84432; BAA23495.1; ALT_SEQ. DR EMBL; Z99116; CAB14333.2; -. DR PIR; E69595; E69595. DR PDB; 1BOW; X-ray; A=120-278. DR PDB; 1EXI; X-ray; A=1-278. DR PDB; 1EXJ; X-ray; A=1-278. DR PDB; 2BOW; X-ray; A=120-278. DR SubtiList; BG10304; bmrR. DR InterPro; IPR000551; HTH_MerR. DR Pfam; PF00376; MerR; 1. DR PROSITE; PS00552; HTH_MERR_1; 1. DR PROSITE; PS50937; HTH_MERR_2; 1. KW 3D-structure; Activator; Complete proteome; DNA-binding; KW Transcription regulation. FT DOMAIN 5 75 HTH merR-type. FT DNA_BIND 8 27 H-T-H motif (Potential). FT STRAND 5 7 FT HELIX 8 14 FT TURN 15 16 FT HELIX 19 28 FT TURN 29 29 FT STRAND 35 36 FT TURN 38 40 FT STRAND 43 46 FT TURN 47 47 FT HELIX 48 51 FT HELIX 52 62 FT TURN 63 63 FT HELIX 66 74 FT HELIX 77 116 FT TURN 117 117 FT STRAND 121 121 FT TURN 122 122 FT STRAND 124 128 FT STRAND 133 138 FT TURN 144 146 FT TURN 150 152 FT HELIX 153 163 FT STRAND 171 174 FT TURN 182 184 FT STRAND 189 193 FT TURN 205 206 FT STRAND 208 211 FT STRAND 216 223 FT HELIX 226 242 FT TURN 243 244 FT STRAND 247 247 FT STRAND 251 253 FT STRAND 256 257 FT STRAND 268 276 SQ SEQUENCE 278 AA; 32582 MW; 184AEF0274991E73 CRC64; MKESYYSIGE VSKLANVSIK ALRYYDKIDL FKPAYVDPDT SYRYYTDSQL IHLDLIKSLK YIGTPLEEMK KAQDLEMEEL FAFYTEQERQ IREKLDFLSA LEQTISLVKK RMKRQMEYPA LGEVFVLDEE EIRIIQTEAE GIGPENVLNA SYSKLKKFIE SADGFTNNSY GATFSFQPYT SIDEMTYRHI FTPVLTNKQI SSITPDMEIT TIPKGRYACI AYNFSPEHYF LNLQKLIKYI ADRQLTVVSD VYELIIPIHY SPKKQEEYRV EMKIRIAE //