ID   BMR2_BACSU     STANDARD;      PRT;   400 AA.
AC   P39843;
DT   01-FEB-1995 (Rel. 31, Created)
DT   01-FEB-1995 (Rel. 31, Last sequence update)
DT   01-FEB-2005 (Rel. 46, Last annotation update)
DE   Multidrug resistance protein 2 (Multidrug-efflux transporter 2).
GN   Name=blt; Synonyms=bmr2, bmt; OrderedLocusNames=BSU26590;
OS   Bacillus subtilis.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1423;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=168 / BD170;
RX   MEDLINE=95332191; PubMed=7608059;
RA   Ahmed M., Lyass L., Markham P.N., Taylor S.S., Vazquez-Laslop N.,
RA   Neyfakh A.A.;
RT   "Two highly similar multidrug transporters of Bacillus subtilis whose
RT   expression is differentially regulated.";
RL   J. Bacteriol. 177:3904-3910(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=168 / JH642;
RX   MEDLINE=97124195; PubMed=8969508;
RA   Mizuno M., Masuda S., Takemaru K.-I., Hosono S., Sato T., Takeuchi M.,
RA   Kobayashi Y.;
RT   "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of
RT   the Bacillus subtilis genome containing the skin element and many
RT   sporulation genes.";
RL   Microbiology 142:3103-3111(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   MEDLINE=98044033; PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA   Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA   Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA   Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA   Choi S.K., Codani J.J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA   Denizot F., Devine K.M., Dusterhoft A., Ehrlich S.D., Emmerson P.T.,
RA   Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA   Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA   Ghim S.Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA   Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA   Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA   Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA   Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA   Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA   Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA   Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA   Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA   Shin B.S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA   Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA   Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA   Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA   Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
CC   -!- FUNCTION: Energy-dependent efflux pump responsible for decreased
CC       drug accumulation in multi-drug-resistant cells. Probably uses a
CC       transmembrane proton gradient as the energy source. Causes the
CC       efflux of a variety of toxic substances, including such
CC       structurally diverse compounds as ethidium bromide, rhodamine and
CC       acridine dyes, tetraphenylphosphonium, puromycin, chloramphenicol,
CC       doxorubicin, and fluoroquinolone antibiotics.
CC   -!- SUBCELLULAR LOCATION: Integral membrane protein.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. TCR/tet
CC       family.
CC   --------------------------------------------------------------------------
CC   This Swiss-Prot entry is copyright. It is produced through a collaboration
CC   between  the Swiss Institute of Bioinformatics  and the  EMBL outstation -
CC   the European Bioinformatics Institute.  There are no  restrictions on  its
CC   use as long as its content is in no way modified and this statement is not
CC   removed.
CC   --------------------------------------------------------------------------
DR   EMBL; L32599; AAC36944.1; -.
DR   EMBL; D84432; BAA12355.1; -.
DR   EMBL; Z99117; CAB14600.1; -.
DR   PIR; I39792; I39792.
DR   SubtiList; BG10905; blt.
DR   InterPro; IPR004734; Drug_resist.
DR   InterPro; IPR007114; MFS.
DR   InterPro; IPR005828; Sub_transporter.
DR   InterPro; IPR005829; Sug_transporter.
DR   InterPro; IPR001958; TCR_TetA.
DR   InterPro; IPR001411; TCR_TetB.
DR   Pfam; PF00083; Sugar_tr; 1.
DR   PRINTS; PR01035; TCRTETA.
DR   PRINTS; PR01036; TCRTETB.
DR   TIGRFAMs; TIGR00880; 2_A_01_02; 1.
DR   PROSITE; PS50850; MFS; 1.
DR   PROSITE; PS00216; SUGAR_TRANSPORT_1; UNKNOWN_1.
KW   Antibiotic resistance; Complete proteome; Transmembrane; Transport.
FT   TRANSMEM     11     31       Potential.
FT   TRANSMEM     46     66       Potential.
FT   TRANSMEM     78     98       Potential.
FT   TRANSMEM    106    126       Potential.
FT   TRANSMEM    142    162       Potential.
FT   TRANSMEM    164    184       Potential.
FT   TRANSMEM    213    233       Potential.
FT   TRANSMEM    253    273       Potential.
FT   TRANSMEM    297    317       Potential.
FT   TRANSMEM    346    366       Potential.
FT   TRANSMEM    368    388       Potential.
SQ   SEQUENCE   400 AA;  43424 MW;  7F3A6EEC344CD4E7 CRC64;
     MKKSINEQKT IFIILLSNIF VAFLGIGLII PVMPSFMKIM HLSGSTMGYL VAAFAISQLI
     TSPFAGRWVD RFGRKKMIIL GLLIFSLSEL IFGLGTHVSI FYFSRILGGV SAAFIMPAVT
     AYVADITTLK ERSKAMGYVS AAISTGFIIG PGAGGFIAGF GIRMPFFFAS AIALIAAVTS
     VFILKESLSI EERHQLSSHT KESNFIKDLK RSIHPVYFIA FIIVFVMAFG LSAYETVFSL
     FSDHKFGFTP KDIAAIITIS SIVAVVIQVL LFGKLVNKLG EKRMIQLCLI TGAILAFVST
     VMSGFLTVLL VTCFIFLAFD LLRPALTAHL SNMAGNQQGF VAGMNSTYTS LGNIFGPALG
     GILFDLNIHY PFLFAGFVMI VGLGLTMVWK EKKNDAAALN
//