ID APPA_BACSU STANDARD; PRT; 543 AA. AC P42061; DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1995, sequence version 1. DT 07-MAR-2006, entry version 46. DE Oligopeptide-binding protein appA precursor. GN Name=appA; OrderedLocusNames=BSU11380; OS Bacillus subtilis. OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. OX NCBI_TaxID=1423; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=168; RX MEDLINE=95089678; PubMed=7997159; RA Koide A., Hoch J.A.; RT "Identification of a second oligopeptide transport system in Bacillus RT subtilis and determination of its role in sporulation."; RL Mol. Microbiol. 13:417-426(1994). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=168; RX MEDLINE=98044033; PubMed=9384377; DOI=10.1038/36786; RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., RA Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., RA Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., RA Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M., RA Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., RA Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., RA Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., RA Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., RA Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., RA Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., RA Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., RA Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., RA Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., RA Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., RA Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., RA Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., RA Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., RA Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., RA Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., RA Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., RA Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., RA Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., RA Yoshikawa H., Danchin A.; RT "The complete genome sequence of the Gram-positive bacterium Bacillus RT subtilis."; RL Nature 390:249-256(1997). CC -!- FUNCTION: This protein is a component of an oligopeptide permease, CC a binding protein-dependent transport system. This APP system can CC completely substitute for the OPP system in both sporulation and CC genetic competence. AppA can bind and transport tetra- and CC pentapeptides but not tripeptides. CC -!- SUBCELLULAR LOCATION: Bacterial cell membrane; lipid-anchor CC (Probable). CC -!- SIMILARITY: Belongs to the bacterial solute-binding protein 5 CC family. CC ----------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution-NoDerivs License CC ----------------------------------------------------------------------- DR EMBL; U20909; AAA62358.1; -; Genomic_DNA. DR EMBL; Z99110; CAB12995.1; -; Genomic_DNA. DR PIR; I40545; I40545. DR PDB; 1XOC; X-ray; A=25-543. DR SubtiList; BG11087; appA. DR GenomeReviews; AL009126_GR; BSU11380. DR BioCyc; BSUB1423:BSU1139-MONOMER; -. DR InterPro; IPR000914; SBP_bac_5. DR Pfam; PF00496; SBP_bac_5; 1. DR PROSITE; PS00013; PROKAR_LIPOPROTEIN; 1. DR PROSITE; PS01040; SBP_BACTERIAL_5; 1. KW 3D-structure; Competence; Complete proteome; Lipoprotein; Membrane; KW Palmitate; Peptide transport; Protein transport; Signal; Sporulation; KW Transport. FT SIGNAL 1 23 Potential. FT CHAIN 24 543 Oligopeptide-binding protein appA. FT /FTId=PRO_0000031791. FT LIPID 24 24 N-palmitoyl cysteine (Probable). FT LIPID 24 24 S-diacylglycerol cysteine (Probable). FT STRAND 46 53 FT STRAND 58 58 FT TURN 60 62 FT STRAND 65 65 FT HELIX 66 75 FT STRAND 79 82 FT TURN 84 85 FT STRAND 86 86 FT STRAND 88 99 FT HELIX 100 102 FT TURN 103 103 FT STRAND 104 109 FT STRAND 111 112 FT STRAND 114 114 FT TURN 116 117 FT STRAND 118 118 FT STRAND 120 120 FT HELIX 123 134 FT STRAND 135 135 FT TURN 136 137 FT STRAND 141 141 FT HELIX 143 145 FT TURN 146 148 FT STRAND 149 156 FT TURN 157 158 FT STRAND 159 166 FT TURN 169 169 FT HELIX 170 173 FT TURN 174 174 FT HELIX 175 178 FT HELIX 184 187 FT TURN 188 189 FT STRAND 190 190 FT HELIX 192 194 FT TURN 195 197 FT STRAND 198 198 FT HELIX 199 201 FT TURN 202 203 FT STRAND 208 217 FT TURN 218 220 FT STRAND 221 226 FT TURN 228 229 FT TURN 231 232 FT STRAND 236 244 FT STRAND 246 246 FT HELIX 248 256 FT TURN 257 258 FT STRAND 259 259 FT STRAND 262 264 FT HELIX 267 273 FT TURN 274 275 FT STRAND 276 276 FT TURN 277 278 FT STRAND 279 285 FT STRAND 287 294 FT TURN 296 297 FT STRAND 298 298 FT HELIX 299 301 FT HELIX 304 312 FT TURN 313 313 FT HELIX 316 323 FT STRAND 324 324 FT TURN 325 326 FT STRAND 328 330 FT STRAND 333 335 FT TURN 337 338 FT STRAND 339 339 FT TURN 340 341 FT STRAND 342 342 FT TURN 344 345 FT HELIX 355 364 FT TURN 365 366 FT STRAND 368 368 FT STRAND 370 375 FT STRAND 377 378 FT TURN 379 380 FT STRAND 381 382 FT STRAND 384 390 FT TURN 391 392 FT HELIX 394 408 FT TURN 409 411 FT STRAND 413 419 FT HELIX 421 428 FT STRAND 429 429 FT TURN 430 432 FT STRAND 436 443 FT STRAND 446 447 FT TURN 450 450 FT HELIX 451 454 FT STRAND 455 455 FT HELIX 456 458 FT TURN 459 461 FT STRAND 462 463 FT TURN 465 466 FT HELIX 471 480 FT TURN 481 482 FT STRAND 485 485 FT HELIX 486 503 FT STRAND 505 511 FT STRAND 513 519 FT TURN 520 521 FT STRAND 522 524 FT STRAND 529 530 FT TURN 532 535 FT HELIX 536 538 FT STRAND 540 541 SQ SEQUENCE 543 AA; 61947 MW; 66A179D6376934C5 CRC64; MKRRKTALMM LSVLMVLAIF LSACSGSKSS NSSAKKSAGK PQQGGDLVVG SIGEPTLFNS LYSTDDASTD IENMLYSFLT KTDEKLNVKL SLAESIKELD GGLAYDVKIK KGVKFHDGKE LTADDVVFTY SVPLSKDYKG ERGSTYEMLK SVEKKGDYEV LFKLKYKDGN FYNNALDSTA ILPKHILGNV PIADLEENEF NRKKPIGSGP FKFKEWKQGQ YIKLEANDDY FEGRPYLDTV TYKVIPDANA AEAQLQAGDI NFFNVPATDY KTAEKFNNLK IVTDLALSYV YIGWNEKNEL FKDKKVRQAL TTALDRESIV SQVLDGDGEV AYIPESPLSW NYPKDIDVPK FEYNEKKAKQ MLAEAGWKDT NGDGILDKDG KKFSFTLKTN QGNKVREDIA VVVQEQLKKI GIEVKTQIVE WSALVEQMNP PNWDFDAMVM GWSLSTFPDQ YDIFHSSQIK KGLNYVWYKN AEADKLMKDA KSISDRKQYS KEYEQIYQKI AEDQPYTFLY YPNNHMAMPE NLEGYKYHPK RDLYNIEKWW LAK //