ID   ADH2_BACST     STANDARD;      PRT;   339 AA.
AC   P42327;
DT   01-NOV-1995 (Rel. 32, Created)
DT   01-NOV-1995 (Rel. 32, Last sequence update)
DT   05-JUL-2004 (Rel. 44, Last annotation update)
DE   Alcohol dehydrogenase (EC 1.1.1.1) (ADH).
GN   Name=adh;
OS   Bacillus stearothermophilus.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Geobacillus.
OX   NCBI_TaxID=1422;
RN   [1]
RP   NUCLEOTIDE SEQUENCE, AND PROTEIN SEQUENCE OF 1-40.
RC   STRAIN=DSM 2334;
RX   MEDLINE=94325354; PubMed=8049268; DOI=10.1016/0167-4781(94)90199-6;
RA   Robinson G.A., Bailey C.J., Dowds B.C.A.;
RT   "Gene structure and amino acid sequences of alcohol dehydrogenases of
RT   Bacillus stearothermophilus.";
RL   Biochim. Biophys. Acta 1218:432-434(1994).
CC   -!- FUNCTION: Active with primary alcohols, including methanol.
CC   -!- CATALYTIC ACTIVITY: An alcohol + NAD(+) = an aldehyde or ketone +
CC       NADH.
CC   -!- COFACTOR: Binds 2 zinc ions per subunit (By similarity).
CC   -!- ENZYME REGULATION: The rate-limiting step is NADH release.
CC       Catabolite repression.
CC   -!- SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase
CC       family.
CC   --------------------------------------------------------------------------
CC   This Swiss-Prot entry is copyright. It is produced through a collaboration
CC   between  the Swiss Institute of Bioinformatics  and the  EMBL outstation -
CC   the European Bioinformatics Institute.  There are no  restrictions on  its
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DR   EMBL; Z25544; CAA80989.1; -.
DR   PIR; S47643; S47643.
DR   HSSP; P39462; 1JVB.
DR   InterPro; IPR002328; ADH_zinc.
DR   InterPro; IPR002085; Adh_zn_family.
DR   InterPro; IPR011032; GroES_like.
DR   Pfam; PF00107; ADH_zinc_N; 1.
DR   PROSITE; PS00059; ADH_ZINC; 1.
KW   Direct protein sequencing; Metal-binding; NAD; Oxidoreductase; Zinc.
FT   METAL        38     38       Zinc 1 (catalytic) (By similarity).
FT   METAL        61     61       Zinc 1 (catalytic) (By similarity).
FT   METAL        92     92       Zinc 2 (By similarity).
FT   METAL        95     95       Zinc 2 (By similarity).
FT   METAL        98     98       Zinc 2 (By similarity).
FT   METAL       106    106       Zinc 2 (By similarity).
FT   METAL       148    148       Zinc 1 (catalytic) (By similarity).
SQ   SEQUENCE   339 AA;  36205 MW;  0EC33CE7287D7476 CRC64;
     MKAAVVNEFK KALEIKEVER PKLEEGEVLV KIEACGVCHT DLHAAHGDWP IKPKLPLIPG
     HEGVGIVVEV AKGVKSIKVG DRVGIPWLYS ACGECEYCLT GQETLCPHQL NGGYSVDGGY
     AEYCKAPADY VAKIPDNLDP VEVAPILCAG VTTYKALKVS GARPGEWVAI YGIGGLGHIA
     LQYAKAMGLN VVAVDISDEK SKLAKDLGAD IAINGLKEDP VKAIHDQVGG VHAAISVAVN
     KKAFEQAYQS VKRGGTLVVV GLPNADLPIP IFDTVLNGVS VKGSIVGTRK DMQEALDFAA
     RGKVRPIVET AELEEINEVF ERMEKGKING RIVLKLKED
//