ID   ADH3_BACST     STANDARD;      PRT;   339 AA.
AC   P42328;
DT   01-NOV-1995 (Rel. 32, Created)
DT   01-NOV-1995 (Rel. 32, Last sequence update)
DT   29-MAR-2004 (Rel. 43, Last annotation update)
DE   Alcohol dehydrogenase (EC 1.1.1.1) (ADH-HT).
OS   Bacillus stearothermophilus.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Geobacillus.
OX   NCBI_TaxID=1422;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=NCIMB 12403 / LLD-R;
RX   MEDLINE=94291628; PubMed=8020473;
RA   Cannio R., Rossi M., Bartolucci S.;
RT   "A few amino acid substitutions are responsible for the higher
RT   thermostability of a novel NAD(+)-dependent bacillar alcohol
RT   dehydrogenase.";
RL   Eur. J. Biochem. 222:345-352(1994).
CC   -!- FUNCTION: Thermostable and thermophilic NAD(+)-dependent alcohol
CC       dehydrogenase. Bears mainly an ethanol-dehydrogenase activity.
CC   -!- CATALYTIC ACTIVITY: An alcohol + NAD(+) = an aldehyde or ketone +
CC       NADH.
CC   -!- COFACTOR: Binds 2 zinc ions per subunit (By similarity).
CC   -!- SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase
CC       family.
CC   --------------------------------------------------------------------------
CC   This Swiss-Prot entry is copyright. It is produced through a collaboration
CC   between  the Swiss Institute of Bioinformatics  and the  EMBL outstation -
CC   the European Bioinformatics Institute.  There are no  restrictions on  its
CC   use as long as its content is in no way modified and this statement is not
CC   removed.
CC   --------------------------------------------------------------------------
DR   EMBL; Z27089; CAA81612.1; -.
DR   PIR; S45605; S45605.
DR   HSSP; P00326; 1HT0.
DR   InterPro; IPR002328; ADH_zinc.
DR   InterPro; IPR002085; Adh_zn_family.
DR   InterPro; IPR011032; GroES_like.
DR   Pfam; PF00107; ADH_zinc_N; 1.
DR   PROSITE; PS00059; ADH_ZINC; 1.
KW   Metal-binding; NAD; Oxidoreductase; Zinc.
FT   METAL        38     38       Zinc 1 (catalytic) (By similarity).
FT   METAL        61     61       Zinc 1 (catalytic) (By similarity).
FT   METAL        92     92       Zinc 2 (By similarity).
FT   METAL        95     95       Zinc 2 (By similarity).
FT   METAL        98     98       Zinc 2 (By similarity).
FT   METAL       106    106       Zinc 2 (By similarity).
FT   METAL       148    148       Zinc 1 (catalytic) (By similarity).
SQ   SEQUENCE   339 AA;  36338 MW;  AED17E4A34163430 CRC64;
     MKAAVVEQFK EPLKIKEVEK PTISYGEVLV RIKACGVCHT DLHAAHGDWP VKPKLPLIPG
     HEGVGIVEEV GPGVTHLKVG DRVGIPWLYS ACGHCDYCLS GQETLCEHQK NAGYSVDGGY
     AEYCRAAADY VVKIPDNLSF EEAAPIFCAG VTTYKALKVT GAKPGEWVAI YGIGGLGHVA
     VQYAKAMGLN VVAVDIGDEK LELAKELGAD LVVNPLKEDA AKFMKEKVGG VHAAVVTAVS
     KPAFQSAYNS IRRGGACVLV GLPPEEMPIP IFDTVLNGIK IIGSIVGTRK DLQEALQFAA
     EGKVKTIIEV QPLEKINEVF DRMLKGQING RVVLTLEDK
//