ID   ALR2_BACSU     STANDARD;      PRT;   394 AA.
AC   P94494;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   21-MAR-2006, entry version 47.
DE   Alanine racemase 2 (EC 5.1.1.1).
GN   Name=alr2; OrderedLocusNames=BSU17640;
OS   Bacillus subtilis.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1423;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Borchert S., Klein C., Piksa B., Hammelmann M., Entian K.-D.;
RT   "Sequencing of a 26 kb region of the Bacillus subtilis genome
RT   downstream of spoVJ.";
RL   Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   MEDLINE=98044033; PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA   Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA   Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA   Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA   Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA   Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA   Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA   Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA   Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA   Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA   Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA   Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA   Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA   Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA   Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA   Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA   Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA   Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA   Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA   Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA   Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA   Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
CC   -!- FUNCTION: Provides the D-alanine required for cell wall
CC       biosynthesis (By similarity).
CC   -!- CATALYTIC ACTIVITY: L-alanine = D-alanine.
CC   -!- COFACTOR: Pyridoxal phosphate (By similarity).
CC   -!- PATHWAY: D-alanine branch of peptidoglycan biosynthesis; first
CC       step.
CC   -!- SIMILARITY: Belongs to the alanine racemase family.
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DR   EMBL; U66480; AAB41097.1; -; Genomic_DNA.
DR   EMBL; Z99113; CAB13648.1; -; Genomic_DNA.
DR   PIR; F69888; F69888.
DR   HSSP; P10724; 1BD0.
DR   SubtiList; BG12267; alr2.
DR   GenomeReviews; AL009126_GR; BSU17640.
DR   BioCyc; BSUB1423:BSU1765-MONOMER; -.
DR   HAMAP; MF_01201; -; 1.
DR   InterPro; IPR012155; Ala_racemase.
DR   InterPro; IPR011079; Ala_racemase_C.
DR   InterPro; IPR001608; Ala_racemase_N.
DR   InterPro; IPR000821; Ala_racemase_reg.
DR   InterPro; IPR009006; Racem_decarbox_C.
DR   Pfam; PF00842; Ala_racemase_C; 1.
DR   Pfam; PF01168; Ala_racemase_N; 1.
DR   PIRSF; PIRSF001401; Ala_racemase; 1.
DR   PRINTS; PR00992; ALARACEMASE.
DR   TIGRFAMs; TIGR00492; alr; 1.
DR   PROSITE; PS00395; ALANINE_RACEMASE; 1.
KW   Cell shape; Cell wall; Complete proteome; Isomerase;
KW   Peptidoglycan synthesis; Pyridoxal phosphate.
FT   CHAIN         1    394       Alanine racemase 2.
FT                                /FTId=PRO_0000114501.
FT   ACT_SITE     39     39       Proton acceptor; specific for D-alanine
FT                                (By similarity).
FT   ACT_SITE    272    272       Proton acceptor; specific for L-alanine
FT                                (By similarity).
FT   BINDING      39     39       Pyridoxal phosphate (covalent) (By
FT                                similarity).
SQ   SEQUENCE   394 AA;  43648 MW;  FE446C21107FBA6E CRC64;
     MIKLCREVWI EVNLDAVKKN LRAIRRHIPH KSKIMAVVKA NGYGHGSIEV ARHALEHGAS
     ELAVASVEEG IVLRKAGITA PILVLGFTSL SCVKKSAAWN ITLSAFQVDW MKEANEILEK
     EASANRLAIH INVDTGMGRL GVRTKEELLE VVKALKASKF LRWTGIFTHF STADEPDTTL
     TKLQHEKFIS FLSFLKKQGI ELPTVHMCNT AAAIAFPEFS ADMIRLGIGL YGLYPSAYIK
     QLNLVKLEPA LSLKARIAYV KTMRTEPRTV SYGATYIAEP NEVIATLPIG YADGYSRALS
     NRGFVLHRGK RVPVAGRVTM DMIMVSLGEN GEGKQGDEVV IYGKQKGAEI SVDEVAEMLN
     TINYEVVSTL SRRIPRFYIR DGEIFKVSTP VLYV
//