ID   AMAB2_BACST    STANDARD;      PRT;   409 AA.
AC   Q53389;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   02-MAY-2006, entry version 29.
DE   N-carbamoyl-L-amino acid hydrolase (EC 3.5.1.87) (L-carbamoylase).
GN   Name=amaB;
OS   Bacillus stearothermophilus (Geobacillus stearothermophilus).
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Geobacillus.
OX   NCBI_TaxID=1422;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NS1122A;
RX   MEDLINE=94115050; PubMed=7764340;
RA   Mukohara Y., Ishikawa T., Watabe K., Nakamura H.;
RT   "Molecular cloning and sequencing of the gene for a thermostable
RT   N-carbamyl-L-amino acid amidohydrolase from Bacillus
RT   stearothermophilus strain NS1122A.";
RL   Biosci. Biotechnol. Biochem. 57:1935-1937(1993).
CC   -!- CATALYTIC ACTIVITY: N-carbamoyl-L-2-amino acid (a 2-ureido
CC       carboxylate) + H(2)O = L-2-amino acid + NH(3) + CO(2).
CC   -!- COFACTOR: Manganese or nickel or cobalt.
CC   -!- SIMILARITY: Belongs to the peptidase M20 family.
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DR   EMBL; S67784; AAC60456.1; -; Genomic_DNA.
DR   PIR; JN0885; JN0885.
DR   InterPro; IPR010158; Hydantase.
DR   InterPro; IPR011650; M20_dimer.
DR   InterPro; IPR002933; Peptidase_M20.
DR   Pfam; PF07687; M20_dimer; 1.
DR   Pfam; PF01546; Peptidase_M20; 1.
DR   TIGRFAMs; TIGR01879; hydantase; 1.
KW   Hydrolase.
FT   CHAIN         1    409       N-carbamoyl-L-amino acid hydrolase.
FT                                /FTId=PRO_0000061953.
SQ   SEQUENCE   409 AA;  44249 MW;  332515C62A1A2F31 CRC64;
     MIQGERLWQR LMELGEVGKQ PSGGVTRLSF TAEERRAKDL VASYMREAGL FVYEDAAGNL
     IGRKEGTNPD ATVVLVGSHL DSVYNGGCFD GPLGVLAGVE VVQTMNEHGV VTHHPIEVVA
     FTDEEGARFR FGMIGSRAMA GTLPPEALEC RDAEGISLAE AMKQAGLDPD RLPQAARKPG
     TVKAYVELHI EQGRVLEETG LPVGIVTGIA GLIWVKFTIE GKAEHAGATP MSLRRDPMAA
     AAQIIIVIEE EARRTGTTVG TVGQLHVYPG GINVIPERVE FVLDLRDLKA EVRDQVWKAI
     AVRAETIAKE RNVRVTTERL QEMPPVLCSD EVKRAAEAAC QKLGYPSFWL PSGAAHDSVQ
     LAPICPIGMI FVRSQDGVSH SPAEWSTKED CAAGAEVLYH TVWQLAQGE
//