ID   ALR_BACPS      STANDARD;      PRT;   383 AA.
AC   Q9S5V6; O82941;
DT   05-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   21-MAR-2006, entry version 35.
DE   Alanine racemase (EC 5.1.1.1).
GN   Name=alr;
OS   Bacillus psychrosaccharolyticus.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1407;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-50.
RX   MEDLINE=99217424; PubMed=10080917; DOI=10.1006/bbrc.1999.0324;
RA   Okubo Y., Yokoigawa K., Esaki N., Soda K., Kawai H.;
RT   "Characterization of psychrophilic alanine racemase from Bacillus
RT   psychrosaccharolyticus.";
RL   Biochem. Biophys. Res. Commun. 256:333-340(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 48-166.
RA   Okubo Y., Yokoigawa K., Kawai H.;
RT   "Alanine racemase gene fragments as probes for detecting Bacillus
RT   stearothermophilus and Bacillus psychrosaccharolyticus in foods.";
RL   J. Ferment. Bioeng. 85:559-563(1998).
CC   -!- FUNCTION: Provides the D-alanine required for cell wall
CC       biosynthesis.
CC   -!- CATALYTIC ACTIVITY: L-alanine = D-alanine.
CC   -!- COFACTOR: Pyridoxal phosphate.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 8-10;
CC       Temperature dependence:
CC         Highly active at low temperatures, even at 0 degree Celsius.
CC         Thermolabile;
CC   -!- PATHWAY: D-alanine branch of peptidoglycan biosynthesis; first
CC       step.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Belongs to the alanine racemase family.
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DR   EMBL; AB021683; BAA76373.1; -; Genomic_DNA.
DR   EMBL; AB011668; BAA31255.1; -; Genomic_DNA.
DR   HSSP; P10724; 1BD0.
DR   HAMAP; MF_01201; -; 1.
DR   InterPro; IPR012155; Ala_racemase.
DR   InterPro; IPR011079; Ala_racemase_C.
DR   InterPro; IPR001608; Ala_racemase_N.
DR   InterPro; IPR000821; Ala_racemase_reg.
DR   InterPro; IPR009006; Racem_decarbox_C.
DR   Pfam; PF00842; Ala_racemase_C; 1.
DR   Pfam; PF01168; Ala_racemase_N; 1.
DR   PIRSF; PIRSF001401; Ala_racemase; 1.
DR   PRINTS; PR00992; ALARACEMASE.
DR   TIGRFAMs; TIGR00492; alr; 1.
DR   PROSITE; PS00395; ALANINE_RACEMASE; 1.
KW   Cell shape; Cell wall; Direct protein sequencing; Isomerase;
KW   Peptidoglycan synthesis; Pyridoxal phosphate.
FT   CHAIN         1    383       Alanine racemase.
FT                                /FTId=PRO_0000114498.
FT   ACT_SITE     41     41       Proton acceptor; specific for D-alanine
FT                                (By similarity).
FT   ACT_SITE    266    266       Proton acceptor; specific for L-alanine
FT                                (By similarity).
FT   BINDING      41     41       Pyridoxal phosphate (covalent) (By
FT                                similarity).
SQ   SEQUENCE   383 AA;  42519 MW;  E6DE9421EEFC5BF7 CRC64;
     MTLQNFYRDT WAEINLDAIF ENAANMKKHL PPEITLFAVV KANAYGHGDV EVAETAIQAG
     AGYLAVAFLD EALALRKKGI TAPILVLGAS RPEDAQIAAR ESITLTVFQA GWLEAAQSFL
     EGTVLTIHLK CDSGMGRIGI RKQEEMNEIE RFLQKTKCFV LEGIFTHFAT ADQLDTEYFS
     KQLARFEEML TWLKEKPKYV HAANSAALLR FPNAVFNSVR MGISLYGLSP SMEMKEVLPF
     ALRPAFSLKT KLVHVKNISK GQSVSYGATY TAEEDTWIGT LPIGYADGWI RMLQGQEVLL
     EGGRSPLVGR ICMDQCMVKL SREFPVGTEV TLIGKNGTEC ITVDDIAEKL NTINYEVTCM
     ISSRVPRMYL KDGRITGVVN QLI
//