ID   BPHF_BURCE     STANDARD;      PRT;   109 AA.
AC   P37332;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 2.
DT   07-FEB-2006, entry version 36.
DE   Biphenyl dioxygenase system ferredoxin subunit.
GN   Name=bphF;
OS   Burkholderia cepacia (Pseudomonas cepacia).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX   NCBI_TaxID=292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=LB400;
RX   MEDLINE=92234948; PubMed=1569021;
RA   Erickson B.D., Mondello F.J.;
RT   "Nucleotide sequencing and transcriptional mapping of the genes
RT   encoding biphenyl dioxygenase, a multicomponent polychlorinated-
RT   biphenyl-degrading enzyme in Pseudomonas strain LB400.";
RL   J. Bacteriol. 174:2903-2912(1992).
RN   [2]
RP   SEQUENCE REVISION.
RA   Erickson B.D., Mondello F.J.;
RL   Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   CHARACTERIZATION.
RX   MEDLINE=20580187; PubMed=11141059; DOI=10.1021/bi001780r;
RA   Couture M.M.-J., Colbert C.L., Babini E., Rosell F.I., Mauk A.G.,
RA   Bolin J.T., Eltis L.D.;
RT   "Characterization of BphF, a Rieske-type ferredoxin with a low
RT   reduction potential.";
RL   Biochemistry 40:84-92(2001).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
RX   MEDLINE=21029088; PubMed=11188691; DOI=10.1016/S0969-2126(00)00536-0;
RA   Colbert C.L., Couture M.M.-J., Eltis L.D., Bolin J.T.;
RT   "A cluster exposed: structure of the Rieske ferredoxin from biphenyl
RT   dioxygenase and the redox properties of Rieske Fe-S proteins.";
RL   Structure 8:1267-1278(2000).
CC   -!- FUNCTION: This protein seems to be a 2Fe-2S ferredoxin.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Redox potential:
CC         E(0) is -157 mV;
CC   -!- SUBUNIT: This dioxygenase system consists of four proteins: the
CC       two subunits of the hydroxylase component (bphA and bphE), a
CC       ferredoxin (bphF) and a ferredoxin reductase (bphG).
CC   -!- SIMILARITY: Belongs to the bacterial ring-hydroxylating
CC       dioxygenase ferredoxin component family.
CC   -!- SIMILARITY: Contains 1 Rieske domain.
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DR   EMBL; M86348; AAB63428.1; -; Genomic_DNA.
DR   PIR; E41858; E41858.
DR   PDB; 1FQT; X-ray; A/B=1-109.
DR   InterPro; IPR005806; Rieske_reg.
DR   Pfam; PF00355; Rieske; 1.
KW   2Fe-2S; 3D-structure; Aromatic hydrocarbons catabolism;
KW   Electron transport; Iron; Iron-sulfur; Metal-binding; Transport.
FT   CHAIN         1    109       Biphenyl dioxygenase system ferredoxin
FT                                subunit.
FT                                /FTId=PRO_0000201686.
FT   DOMAIN        2     96       Rieske.
FT   METAL        43     43       Iron-sulfur (2Fe-2S).
FT   METAL        45     45       Iron-sulfur (2Fe-2S) (via pros nitrogen).
FT   METAL        63     63       Iron-sulfur (2Fe-2S).
FT   METAL        66     66       Iron-sulfur (2Fe-2S) (via pros nitrogen).
FT   STRAND        4      8
FT   HELIX         9     11
FT   STRAND       12     12
FT   TURN         14     15
FT   STRAND       17     22
FT   TURN         23     24
FT   STRAND       25     32
FT   TURN         33     34
FT   STRAND       35     42
FT   TURN         44     45
FT   STRAND       46     47
FT   STRAND       49     51
FT   TURN         52     53
FT   STRAND       56     57
FT   TURN         58     59
FT   STRAND       60     62
FT   TURN         64     66
FT   STRAND       69     71
FT   TURN         72     74
FT   STRAND       77     81
FT   STRAND       84     84
FT   STRAND       88     88
FT   STRAND       91     94
FT   TURN         95     96
FT   STRAND       97    100
FT   TURN        102    103
FT   STRAND      106    106
SQ   SEQUENCE   109 AA;  11955 MW;  39AE9C8B84232FA4 CRC64;
     MKFTRVCDRR DVPEGEALKV ESGGTSVAIF NVDGELFATQ DRCTHGDWSL SDGGYLEGDV
     VECSLHMGKF CVRTGKVKSP PPCEALKIFP IRIEDNDVLV DFEAGYLAP
//