ID GMHA_BURMA STANDARD; PRT; 197 AA. AC Q9AI36; Q62HG3; DT 28-FEB-2003, integrated into UniProtKB/Swiss-Prot. DT 23-NOV-2004, sequence version 2. DT 07-MAR-2006, entry version 23. DE Phosphoheptose isomerase (EC 5.3.1.-) (Sedoheptulose 7-phosphate DE isomerase). GN Name=gmhA; OrderedLocusNames=BMA2295; OS Burkholderia mallei (Pseudomonas mallei). OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; OC Burkholderiaceae; Burkholderia. OX NCBI_TaxID=13373; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 23344; RX MEDLINE=21267073; PubMed=11373120; DOI=10.1006/mpat.2000.0430; RA DeShazer D., Waag D.M., Fritz D.L., Woods D.E.; RT "Identification of a Burkholderia mallei polysaccharide gene cluster RT by subtractive hybridization and demonstration that the encoded RT capsule is an essential virulence determinant."; RL Microb. Pathog. 30:253-269(2001). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 23344; RX PubMed=15377793; DOI=10.1073/pnas.0403306101; RA Nierman W.C., DeShazer D., Kim H.S., Tettelin H., Nelson K.E., RA Feldblyum T.V., Ulrich R.L., Ronning C.M., Brinkac L.M., RA Daugherty S.C., Davidsen T.D., DeBoy R.T., Dimitrov G., Dodson R.J., RA Durkin A.S., Gwinn M.L., Haft D.H., Khouri H.M., Kolonay J.F., RA Madupu R., Mohammoud Y., Nelson W.C., Radune D., Romero C.M., RA Sarria S., Selengut J., Shamblin C., Sullivan S.A., White O., Yu Y., RA Zafar N., Zhou L., Fraser C.M.; RT "Structural flexibility in the Burkholderia mallei genome."; RL Proc. Natl. Acad. Sci. U.S.A. 101:14246-14251(2004). RN [3] RP BIOSYNTHESIS OF NUCLEOTIDE-ACTIVATED GLYCERO-MANNO-HEPTOSE. RX MEDLINE=22095940; PubMed=12101286; RA Valvano M.A., Messner P., Kosma P.; RT "Novel pathways for biosynthesis of nucleotide-activated glycero- RT manno-heptose precursors of bacterial glycoproteins and cell surface RT polysaccharides."; RL Microbiology 148:1979-1989(2002). CC -!- FUNCTION: Catalyzes the isomerization of sedoheptulose 7-phosphate CC in D-glycero-D-manno-heptose 7-phosphate (By similarity). CC -!- CATALYTIC ACTIVITY: D-sedoheptulose 7-phosphate = D-glycero-alpha- CC D-manno-heptose 7-phosphate + D-glycero-beta-D-manno-heptose CC 7-phosphate. CC -!- PATHWAY: Capsular polysaccharide (CPS) biosynthesis; GDP-D- CC glycero-alpha-D-manno-heptose biosynthesis; first step. CC -!- SUBUNIT: Homodimer (By similarity). CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity). CC -!- SIMILARITY: Belongs to the SIS family. GmhA subfamily. CC ----------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution-NoDerivs License CC ----------------------------------------------------------------------- DR EMBL; AF285636; AAK26468.1; -; Genomic_DNA. DR EMBL; CP000010; AAU49838.1; -; Genomic_DNA. DR GenomeReviews; CP000010_GR; BMA2295. DR TIGR; BMA2295; -. DR HAMAP; MF_00067; -; 1. DR InterPro; IPR004515; GmhA. DR TIGRFAMs; TIGR00441; gmhA; 1. KW Bacterial capsule; Carbohydrate metabolism; Complete proteome; KW Isomerase. FT CHAIN 1 197 Phosphoheptose isomerase. FT /FTId=PRO_0000136520. FT CONFLICT 26 26 D -> N (in Ref. 1). SQ SEQUENCE 197 AA; 20784 MW; 63A1B00CA5E440D4 CRC64; MENRELTYIT NSIAEAQRVM AAMLADERLL ATVQKVADAC IASIAQGGKV LLAGNGGSAA DAQHIAGEFV SRFAFDRPGL PAVALTTDTS ILTAIGNDYG YEKLFSRQVQ ALGNKGDVLI GYSTSGKSPN ILAAFREAKA KGMTCVGFTG NRGGEMRELC DLLLEVPSAD TPKIQEGHLV LGHIVCGLVE HSIFGKQ //