ID   BPHE_BURCE     STANDARD;      PRT;   187 AA.
AC   P37334;
DT   01-OCT-1994 (Rel. 30, Created)
DT   28-FEB-2003 (Rel. 41, Last sequence update)
DT   01-FEB-2005 (Rel. 46, Last annotation update)
DE   Biphenyl dioxygenase beta subunit (EC 1.14.12.18) (Biphenyl 2,3-
DE   dioxygenase).
GN   Name=bphE;
OS   Burkholderia cepacia (Pseudomonas cepacia).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia.
OX   NCBI_TaxID=292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=LB400;
RX   MEDLINE=92234948; PubMed=1569021;
RA   Erickson B.D., Mondello F.J.;
RT   "Nucleotide sequencing and transcriptional mapping of the genes
RT   encoding biphenyl dioxygenase, a multicomponent polychlorinated-
RT   biphenyl-degrading enzyme in Pseudomonas strain LB400.";
RL   J. Bacteriol. 174:2903-2912(1992).
RN   [2]
RP   PROTEIN SEQUENCE OF 1-11, AND CHARACTERIZATION.
RC   STRAIN=LB400;
RX   MEDLINE=96011369; PubMed=7592331;
RA   Haddock J.D., Gibson D.T.;
RT   "Purification and characterization of the oxygenase component of
RT   biphenyl 2,3-dioxygenase from Pseudomonas sp. strain LB400.";
RL   J. Bacteriol. 177:5834-5839(1995).
RN   [3]
RP   ERRATUM.
RA   Haddock J.D., Gibson D.T.;
RL   J. Bacteriol. 178:258-258(1996).
CC   -!- FUNCTION: The beta subunit may be responsible for the substrate
CC       specificity of the enzyme.
CC   -!- CATALYTIC ACTIVITY: Biphenyl + NADH + O(2) = (1S,2R)-3-
CC       phenylcyclohexa-3,5-diene-1,2-diol + NAD(+).
CC   -!- PATHWAY: Biphenyl-polychlorinated biphenyl degradation pathway;
CC       first step.
CC   -!- SUBUNIT: Heterohexamer consisting of three BphA subunits and three
CC       BphE subunits. A ferredoxin (BphF) and a ferredoxin reductase
CC       (BphG) must be present to obtain activity.
CC   -!- SIMILARITY: Belongs to the bacterial ring-hydroxylating
CC       dioxygenase beta subunit family.
CC   --------------------------------------------------------------------------
CC   This Swiss-Prot entry is copyright. It is produced through a collaboration
CC   between  the Swiss Institute of Bioinformatics  and the  EMBL outstation -
CC   the European Bioinformatics Institute.  There are no  restrictions on  its
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CC   removed.
CC   --------------------------------------------------------------------------
DR   EMBL; M86348; AAB63426.1; -.
DR   InterPro; IPR000391; Ring_hydroxyl_B.
DR   Pfam; PF00866; Ring_hydroxyl_B; 1.
KW   Aromatic hydrocarbons catabolism; Dioxygenase;
KW   Direct protein sequencing; NAD; Oxidoreductase.
FT   INIT_MET      0      0
SQ   SEQUENCE   187 AA;  21954 MW;  C92B87959FD530A0 CRC64;
     TNPSPHFFKT FEWPSKAAGL ELQNEIEQFY YREAQLLDHR AYEAWFALLD KDIHYFMPLR
     TNRMIREGEL EYSGDQDLAH FDETHETMYG RIRKVTSDVG WAENPPSRTR HLVSNVIVKE
     TATPDTFEVN SAFILYRNRL ERQVDIFAGE RRDVLRRADN NLGFSIAKRT ILLDASTLLS
     NNLSMFF
//