ID   BPHB_BURCE     STANDARD;      PRT;   277 AA.
AC   P47227; Q52435;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   07-MAR-2006, entry version 45.
DE   Cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase (EC 1.3.1.56)
DE   (Biphenyl-2,3-dihydro-2,3-diol dehydrogenase)
DE   (2,3-dihydro-2,3-dihydroxybiphenyl dehydrogenase) (Biphenyl-cis-diol
DE   dehydrogenase) (2,3-dihydroxy-4-phenylhexa-4,6-diene dehydrogenase).
GN   Name=bphB;
OS   Burkholderia cepacia (Pseudomonas cepacia).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX   NCBI_TaxID=292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=LB400;
RX   MEDLINE=93345822; PubMed=8344527; DOI=10.1016/0378-1119(93)90345-4;
RA   Hofer B., Eltis L.D., Dowling D.N., Timmis K.N.;
RT   "Genetic analysis of a Pseudomonas locus encoding a pathway for
RT   biphenyl/polychlorinated biphenyl degradation.";
RL   Gene 130:47-55(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-22.
RC   STRAIN=LB400;
RX   MEDLINE=92234948; PubMed=1569021;
RA   Erickson B.D., Mondello F.J.;
RT   "Nucleotide sequencing and transcriptional mapping of the genes
RT   encoding biphenyl dioxygenase, a multicomponent polychlorinated-
RT   biphenyl-degrading enzyme in Pseudomonas strain LB400.";
RL   J. Bacteriol. 174:2903-2912(1992).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH NAD.
RX   MEDLINE=98318037; PubMed=9655331;
RA   Huelsmeyer M., Hecht H.-J., Niefind K., Hofer B., Eltis L.D.,
RA   Timmis K.N., Schomburg D.;
RT   "Crystal structure of cis-biphenyl-2,3-dihydrodiol-2,3-dehydrogenase
RT   from a PCB degrader at 2.0-A resolution.";
RL   Protein Sci. 7:1286-1293(1998).
CC   -!- CATALYTIC ACTIVITY: Cis-3-phenylcyclohexa-3,5-diene-1,2-diol +
CC       NAD(+) = biphenyl-2,3-diol + NADH.
CC   -!- PATHWAY: Degradation of biphenyls and polychlorobiphenyls (PCB) to
CC       benzoic acid and chlorobenzoic acids; second step.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases
CC       (SDR) family.
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DR   EMBL; X66122; CAA46909.1; -; Genomic_DNA.
DR   EMBL; M86348; AAB63430.2; -; Genomic_DNA.
DR   PIR; JN0814; JN0814.
DR   PDB; 1BDB; X-ray; @=1-277.
DR   LinkHub; P47227; -.
DR   InterPro; IPR002347; ADH_short_C2.
DR   InterPro; IPR002198; SDR.
DR   PANTHER; PTHR19410; ADH_short; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   PROSITE; PS00061; ADH_SHORT; 1.
KW   3D-structure; Aromatic hydrocarbons catabolism; NAD; Oxidoreductase.
FT   CHAIN         1    277       Cis-2,3-dihydrobiphenyl-2,3-diol
FT                                dehydrogenase.
FT                                /FTId=PRO_0000054531.
FT   NP_BIND       9     36       NAD.
FT   ACT_SITE    155    155       Proton acceptor.
FT   BINDING      59     59       NAD.
FT   BINDING     142    142       Substrate (By similarity).
FT   BINDING     159    159       NAD; ribose group.
FT   TURN          2      5
FT   STRAND        7     11
FT   TURN         12     14
FT   STRAND       15     15
FT   HELIX        16     27
FT   TURN         28     29
FT   STRAND       31     37
FT   HELIX        39     52
FT   STRAND       53     57
FT   TURN         60     61
FT   HELIX        63     77
FT   STRAND       78     78
FT   STRAND       82     84
FT   TURN         92     93
FT   HELIX        96     98
FT   STRAND       99     99
FT   TURN        101    103
FT   HELIX       104    115
FT   TURN        116    116
FT   HELIX       117    133
FT   TURN        134    134
FT   STRAND      136    140
FT   HELIX       143    145
FT   TURN        146    146
FT   STRAND      147    147
FT   TURN        148    149
FT   STRAND      150    151
FT   HELIX       153    173
FT   TURN        174    176
FT   STRAND      178    184
FT   STRAND      189    189
FT   HELIX       195    197
FT   HELIX       209    213
FT   TURN        214    216
FT   TURN        218    219
FT   STRAND      220    221
FT   HELIX       225    228
FT   STRAND      229    229
FT   HELIX       230    236
FT   HELIX       238    241
FT   TURN        242    243
FT   STRAND      245    246
FT   STRAND      248    254
FT   HELIX       255    257
FT   STRAND      260    261
FT   STRAND      263    263
FT   STRAND      265    265
FT   TURN        267    268
FT   HELIX       269    273
FT   TURN        274    274
SQ   SEQUENCE   277 AA;  28901 MW;  00194120BD4E12D1 CRC64;
     MKLKGEAVLI TGGASGLGRA LVDRFVAEGA KVAVLDKSAE RLAELETDHG DNVLGIVGDV
     RSLEDQKQAA SRCVARFGKI DTLIPNAGIW DYSTALVDLP EESLDAAFDE VFHINVKGYI
     HAVKACLPAL VASRGNVIFT ISNAGFYPNG GGPLYTAAKH AIVGLVRELA FELAPYVRVN
     GVGSGGINSD LRGPSSLGMG SKAISTVPLA DMLKSVLPIG RMPEVEEYTG AYVFFATRGD
     AAPATGALLN YDGGLGVRGF FSGAGGNDLL EQLNIHP
//