ID   AMPA_BURPS     STANDARD;      PRT;   503 AA.
AC   Q63WC3;
DT   01-FEB-2005 (Rel. 46, Created)
DT   01-FEB-2005 (Rel. 46, Last sequence update)
DT   01-FEB-2005 (Rel. 46, Last annotation update)
DE   Probable cytosol aminopeptidase (EC 3.4.11.1) (Leucine aminopeptidase)
DE   (LAP) (Leucyl aminopeptidase).
GN   Name=pepA; OrderedLocusNames=BPSL0965;
OS   Burkholderia pseudomallei (Pseudomonas pseudomallei).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia.
OX   NCBI_TaxID=28450;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K96243;
RX   PubMed=15377794; DOI=10.1073/pnas.0403302101;
RA   Holden M.T.G., Titball R.W., Peacock S.J., Cerdeno-Tarraga A.-M.,
RA   Atkins T., Crossman L.C., Pitt T., Churcher C., Mungall K.L.,
RA   Bentley S.D., Sebaihia M., Thomson N.R., Bason N., Beacham I.R.,
RA   Brooks K., Brown K.A., Brown N.F., Challis G.L., Cherevach I.,
RA   Chillingworth T., Cronin A., Crossett B., Davis P., DeShazer D.,
RA   Feltwell T., Fraser A., Hance Z., Hauser H., Holroyd S., Jagels K.,
RA   Keith K.E., Maddison M., Moule S., Price C., Quail M.A.,
RA   Rabbinowitsch E., Rutherford K., Sanders M., Simmonds M.,
RA   Songsivilai S., Stevens K., Tumapa S., Vesaratchavest M.,
RA   Whitehead S., Yeats C., Barrell B.G., Oyston P.C.F., Parkhill J.;
RT   "Genomic plasticity of the causative agent of melioidosis,
RT   Burkholderia pseudomallei.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:14240-14245(2004).
CC   -!- FUNCTION: Presumably involved in the processing and regular
CC       turnover of intracellular proteins. Catalyzes the removal of
CC       unsubstituted N-terminal amino acids from various peptides (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: Release of an N-terminal amino acid, Xaa-|-
CC       Yaa-, in which Xaa is preferably Leu, but may be other amino acids
CC       including Pro although not Arg or Lys, and Yaa may be Pro. Amino
CC       acid amides and methyl esters are also readily hydrolyzed, but
CC       rates on arylamides are exceedingly low.
CC   -!- COFACTOR: Binds 2 manganese ions per subunit (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic (By similarity).
CC   -!- SIMILARITY: Belongs to the peptidase M17 family.
CC   --------------------------------------------------------------------------
CC   This Swiss-Prot entry is copyright. It is produced through a collaboration
CC   between  the Swiss Institute of Bioinformatics  and the  EMBL outstation -
CC   the European Bioinformatics Institute.  There are no  restrictions on  its
CC   use as long as its content is in no way modified and this statement is not
CC   removed.
CC   --------------------------------------------------------------------------
DR   EMBL; BX571965; CAH34961.1; -.
DR   HAMAP; MF_00181; -; 1.
DR   InterPro; IPR011356; Peptidase_M17.
DR   InterPro; IPR000819; Peptidase_M17_C.
DR   InterPro; IPR008283; Peptidase_M17_N.
DR   Pfam; PF00883; Peptidase_M17; 1.
DR   Pfam; PF02789; Peptidase_M17_N; 1.
DR   PIRSF; PIRSF001116; Ctsl_amnpptdse; 1.
DR   PRINTS; PR00481; LAMNOPPTDASE.
DR   PROSITE; PS00631; CYTOSOL_AP; 1.
KW   Aminopeptidase; Complete proteome; Hydrolase; Manganese; Protease.
FT   ACT_SITE    286    286       Potential.
FT   ACT_SITE    360    360       Potential.
FT   METAL       274    274       Manganese 2 (By similarity).
FT   METAL       279    279       Manganese 1 and 2 (By similarity).
FT   METAL       297    297       Manganese 2 (By similarity).
FT   METAL       356    356       Manganese 1 (By similarity).
FT   METAL       358    358       Manganese 1 and 2 (By similarity).
SQ   SEQUENCE   503 AA;  52653 MW;  A641EACDE75CEC4F CRC64;
     MDFSIKGCDW SKGTANGFLT GKSDCIVLGV FEAQTLSGAA LDIDEATKGL VSRVIKAGDI
     DGKLGKTLFL HEVSGIGASR VLLVGLGRQD AFSQKAYGDA AKAAWRALLG TKVVQVTFTL
     AQLPVPERAS DWGVRAAILA LRNETYKFTQ MKSKPDAGAP ALKRVVFSVD PADDKAAKVA
     AKQAVALANG MDLTRDLGNL PGNVCTPTYL ANTAKKIAKD WGLKVDVLGL KQIQALKMGS
     FLSVAKGSVE PPQFIVLQYR GAAAKAAPVV LVGKGITFDS GGISLKPGEG MDEMKYDMCG
     AGSVLGTMRA VAEMGLKVNV VAIVPTCENM PAGNANKPGD IVTSMKGLTI EVLNTDAEGR
     LILCDALTYA ERFKPAAVID VATLTGACII ALGHHNTGLF SKDDALAGEL LDASREAGDP
     AWRLPLDDEY QDQLKSNFAD LANIGGRPAG SVTAACFLSR FAENYPWAHL DIAGTAWKSG
     AAKGATGRPV PLLAQFLIDR AGA
//