LOCUS ASNXYNA 2556 bp DNA linear PLN 14-APR-2000 DEFINITION Aspergillus kawachii xynA gene encoding xylanase A. ACCESSION D14847 VERSION D14847.1 GI:285612 KEYWORDS xylanase A. SOURCE Aspergillus kawachii ORGANISM Aspergillus kawachii Eukaryota; Fungi; Ascomycota; Pezizomycotina; Eurotiomycetes; Eurotiales; Trichocomaceae; mitosporic Trichocomaceae; Aspergillus. REFERENCE 1 (bases 1 to 2556) AUTHORS Ito,K., Ikemasu,T. and Ishikawa,T. TITLE Cloning and sequencing of the xynA gene encoding xylanase A of Aspergillus kawachii JOURNAL Biosci. Biotechnol. Biochem. 56 (6), 906-912 (1992) PUBMED 1368254 REFERENCE 2 (sites) AUTHORS MacLeod,A.M., Lindhorst,T., Withers,S.G. and Warren,R.A. TITLE The acid/base catalyst in the exoglucanase/xylanase from Cellulomonas fimi is glutamic acid 127: evidence from detailed kinetic studies of mutants JOURNAL Biochemistry 33 (20), 6371-6376 (1994) PUBMED 7910761 REFERENCE 3 (bases 1 to 2556) AUTHORS Ito,K. TITLE Direct Submission JOURNAL Submitted (30-MAR-1993) Kiyoshi Ito, National Research Institute of Brewing; 3-7-1 Kagamiyama, Higashihiroshima, Hiroshima 739, Japan (Tel:03-3910-6235, Fax:03-3910-6239) COMMENT Submitted (30-MAR-1993) to DDBJ by: Kiyoshi Ito National Research Institute of Brewing, Japan 2-6-30 Takinogawa Kita-ku, Tokyo 114 Japan Phone: 03-3910-6235 Fax: 03-3910-6239. FEATURES Location/Qualifiers source 1..2556 /organism="Aspergillus kawachii" /mol_type="genomic DNA" /strain="IFO4308" /db_xref="taxon:40384" CAAT_signal 105..108 CAAT_signal 156..159 CAAT_signal 443..446 TATA_signal 450..461 TATA_signal 492..497 CDS join(560..803,854..894,954..1002,1062..1183,1235..1380, 1434..1522,1577..1623,1678..1767,1829..1894,1957..2046) /codon_start=1 /product="xylanase A precursor" /protein_id="BAA03575.1" /db_xref="GI:303497" /translation="MVQIKAAALAMLFASHVLSEPIEPRQASVSIDSKFKAHGKKYLG NIGDQYTLTKNSKTPAVIKADFGALTPENSMKWDATEPSRGQFSFSGSDYLVNFAQSN NKLIRGHTLVWHSQLPSWVQAITDKNTLIEVMKNHITTVMQHYKGKIYAWDVVNEIFN EDGSLRDSVFYKVIGDDYVRIAFETARAADPNAKLYINDYNLDSASYPKLAGMVSHVK KWIEAGIPIDGIGSQTHLSAGGGAGISGALNALAGAGTKEIAVTELDIAGASSTDYVE VVEACLDQPKCIGITVWGVADPDSWRSSSTPLLFDSNYNPKPAYTAIANAL" sig_peptide 560..634 mat_peptide join(635..803,854..894,954..1002,1062..1183,1235..1380, 1434..1522,1577..1623,1678..1767,1829..1894,1957..2043) /product="xylanase A" /EC_number="3.2.1.8" exon 560..803 /number=1 intron 804..853 /number=1 exon 854..894 /number=2 intron 895..953 /number=2 exon 954..1002 /number=3 intron 1003..1061 /number=3 exon 1062..1183 /number=4 intron 1184..1234 /number=4 exon 1235..1380 /number=5 intron 1381..1433 /number=5 exon 1434..1522 /number=6 intron 1523..1576 /number=6 exon 1577..1623 /number=7 intron 1624..1677 /number=7 exon 1678..1767 /number=8 intron 1768..1828 /number=8 exon 1829..1894 /number=9 intron 1895..1956 /number=9 exon 1957..2046 /number=10 repeat_region 2075..2092 /rpt_type=inverted ORIGIN 1 ctgcagggct aaactatttt tggggccgag gcagacgctg gaccctgatg gacggacatt 61 agcaggttaa tcggacgacc gtgcaacaag tgtctccact cctgcaattc ctttttgtgt 121 tcctggtggg tagatcagcg ccttccaaat tacaacaata acgtacggtt gcttctcgat 181 gtgtcggcta aggacacagc ctgttctaac caaacatgtg aagaactccg gatagcctgc 241 ctaaacgcca ttattcatcc cttcaggctg attatggttc catcgagtgc catcctcccg 301 gtttgggaaa ttcggtagac agctagatga ggcagatgaa gtcaatcctt tactgtctag 361 acatgtctcg agcgggatgt ttggttccac ggttggttgt gttcctagtt tcacatcctc 421 ttcgcccgga catattgagt ctcgtgggat ataaatactt gaattcctct tctcccttgt 481 agaatttatc ttataatctg tcattccaag atcagttttc agttagctct atcaagaaaa 541 acagtcgttt ttgtccaaca tggttcagat caaggcagct gcactggcga tgcttttcgc 601 tagccatgtg ctttctgaac ctattgaacc ccgtcaggct tcagtgagca tcgacagcaa 661 gttcaaggct cacggaaaga agtatctggg aaacatcggg gatcagtata ccttgaccaa 721 gaactcgaag actccagcag ttatcaaggc cgattttggc gcgttgaccc cagagaatag 781 catgaagtgg gatgccactg agcgtaagga caccatcgtc atcgttgtta ttcagaccta 841 atcagaatta tagccagccg tggacagttc tctttctcgg gatcggatta cctggtacgt 901 atagaatttc ccaaccctct acgataactg tactcgacgc ttacaagacc taggtcaact 961 ttgcccagtc taacaacaag ttgatccgtg gacatacgct gggtaagttt tgactgattg 1021 aactgaaaac tataacaaga cagtgaagct aactcaacca gtgtggcact ctcagctccc 1081 ctcttgggtt caagccatca cggacaagaa taccctcatc gaggtcatga aaaatcacat 1141 caccacggtg atgcaacact ataagggcaa gatctacgcc tgggtaaact gaaaaccatc 1201 aattttgcca agtctcatca ctaattatta caaggacgtt gtgaacgaaa tcttcaacga 1261 agacggctcc ctccgcgaca gcgtgttcta caaagtcatc ggcgacgact acgtccggat 1321 tgccttcgag actgctcggg ctgcggatcc caacgcaaag ctctacatca acgattacaa 1381 gtaagcctga ctgtctaatt accattgcaa agtggacgct aatttccgca aagcctggac 1441 tccgcttcct atcctaaatt ggctggcatg gtcagccatg tcaagaagtg gatcgaagcc 1501 ggtattccca tcgatggaat cggtaaggcc cccgtggtta atgatatccg gctataatta 1561 ctcaccaact tcaaaggctc ccaaacccac ttgagcgctg gtggaggtgc tggaatttct 1621 ggagtaagta ccccctcccc gttattcaac ctgacggtag ctgacagccc cttacaggct 1681 cttaatgctc tcgcaggtgc gggcaccaag gagattgcgg tcaccgagct cgacatcgct 1741 ggcgccagct caaccgacta cgtggaggta ggtcacgcca agccaagtac atggtgtgaa 1801 taactgatac tgatttctgc atccacaggt cgtcgaagcc tgccttgacc agcccaagtg 1861 tatcggtatc accgtgtggg gagttgctga cccggtaaat acccctgcaa cccggatagt 1921 aaaactagcc ttatgacttg agctaatttc attcaggact cctggcgctc cagctccact 1981 cctctgctgt ttgacagcaa ctataacccg aagcctgcat acactgctat cgcaaatgct 2041 ctctagattc ggcaaccact ctgatcgaat tgccgggaag atatagcctt ccgaggtagg 2101 gacgctctgt tttgcccgaa ggaattgtct gccgttcctc cgacaatgtt cttctgtact 2161 cctttcacat gtatccaata caatcgttct ctctaagtgt gcgcacccac agtaggtctg 2221 aaccatgcac ctccagtgta gctctcctta gtataatccc tgtggagtag tttctgccgt 2281 gtaccccacc gctggggccg taggattagt acttaactct gttagtctag accctgatgc 2341 gccgatgtga agcacaagca tcacacgaac tgaaggacat gtctcagata ccccgcccca 2401 taaccataac agccatatat gatctaaggt ccttgtggca cgcaattgga tggacggagc 2461 ggtctggcta gcctctgtgc ctgtactaat aaccatcttg gcattggctg gacattggac 2521 tagtcgttca tgatgtcctc gcttagcatc ctgcag //