ID   GUN_CELUD      STANDARD;      PRT;   359 AA.
AC   P18336;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   21-MAR-2006, entry version 41.
DE   Endoglucanase precursor (EC 3.2.1.4) (Endo-1,4-beta-glucanase)
DE   (Cellulase).
OS   Cellulomonas uda.
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Micrococcineae; Cellulomonadaceae; Cellulomonas.
OX   NCBI_TaxID=1714;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 24-37.
RC   STRAIN=CB4;
RA   Nakamura K., Misawa N., Kitamura K.;
RT   "Sequence of a cellulase gene of Cellulomonas uda CB4.";
RL   J. Biotechnol. 4:247-254(1986).
CC   -!- FUNCTION: The biological conversion of cellulose to glucose
CC       generally requires three types of hydrolytic enzymes: (1)
CC       Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2)
CC       Exocellobiohydrolases that cut the dissaccharide cellobiose from
CC       the nonreducing end of the cellulose polymer chain; (3)
CC       Beta-1,4-glucosidases which hydrolyze the cellobiose and other
CC       short cello-oligosaccharides to glucose.
CC   -!- CATALYTIC ACTIVITY: Endohydrolysis of 1,4-beta-D-glucosidic
CC       linkages in cellulose, lichenin and cereal beta-D-glucans.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 8 (cellulase D)
CC       family.
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DR   EMBL; M36503; AAA23090.1; -; Genomic_DNA.
DR   PIR; I40696; I40696.
DR   InterPro; IPR008928; 6hp_glycosidase.
DR   InterPro; IPR012341; CelA/Cel48F_cat.
DR   InterPro; IPR002037; Glyco_hydro_8.
DR   Pfam; PF01270; Glyco_hydro_8; 1.
DR   PRINTS; PR00735; GLHYDRLASE8.
DR   PROSITE; PS00812; GLYCOSYL_HYDROL_F8; 1.
KW   Carbohydrate metabolism; Cellulose degradation;
KW   Direct protein sequencing; Glycosidase; Hydrolase;
KW   Polysaccharide degradation; Signal.
FT   SIGNAL        1     23
FT   CHAIN        24    359       Endoglucanase.
FT                                /FTId=PRO_0000007933.
FT   ACT_SITE     53     53       Proton donor (By similarity).
FT   ACT_SITE    110    110       Nucleophile (Potential).
SQ   SEQUENCE   359 AA;  40690 MW;  0445D7571B683148 CRC64;
     MPLRALVAVI VTTAVMLVPR AWAQTAWERY KARFMMPDAR IIDTANGNVS HTEGQGFAML
     LAVANNDRPA FDKLWQWTDS TLRDKSNGLF YWRYNPVAPD PIADKNNATD GDTLIAWALL
     RAQKQWQDKR YATASDAITA SLLKYTVVTF AGRQVMLPGV KGFNRNDHLN LNPSYFIFPA
     WRAFAERTHL TAWRTLQSDG QALLGQMGWG KSHLPSDWVA LRADGKMLPA KEWPPRMSFD
     AIRIPLYISW VDPHSALLAP WKAWMQSYPR LQTPAWINVS TNEVAPWNMA GGLLAVRDLT
     LGEPLERRRL TTRMIITPPA SSCWSGWRNR ISASAVMALQ VSQPVCLRAE RKEQERLTM
//