ID   PUNA_CELSP     STANDARD;      PRT;   282 AA.
AC   P81989;
DT   21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2000, sequence version 1.
DT   07-FEB-2006, entry version 35.
DE   Purine nucleoside phosphorylase (EC 2.4.2.1) (Inosine phosphorylase)
DE   (PNP).
GN   Name=punA;
OS   Cellulomonas sp.
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Micrococcineae; Cellulomonadaceae; Cellulomonas.
OX   NCBI_TaxID=40001;
RN   [1]
RP   PROTEIN SEQUENCE, AND X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
RX   MEDLINE=20069945; PubMed=10600382; DOI=10.1006/jmbi.1999.3327;
RA   Tebbe J., Bzowska A., Wielgus-Kutrowska B., Schroeder W.,
RA   Kazimierczuk Z., Shugar D., Saenger W., Koellner G.;
RT   "Crystal structure of the purine nucleoside phosphorylase (PNP) from
RT   Cellulomonas sp. and its implication for the mechanism of trimeric
RT   PNPs.";
RL   J. Mol. Biol. 294:1239-1255(1999).
CC   -!- FUNCTION: Cleavage of guanosine or inosine to respective bases and
CC       sugar-1-phosphate molecules.
CC   -!- CATALYTIC ACTIVITY: Purine nucleoside + phosphate = purine +
CC       alpha-D-ribose 1-phosphate.
CC   -!- PATHWAY: Purine nucleoside salvage.
CC   -!- SUBUNIT: Homotrimer.
CC   -!- SIMILARITY: Belongs to the PNP/MTAP phosphorylase family.
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DR   PDB; 1C3X; X-ray; A/B/C=9-282.
DR   PDB; 1QE5; X-ray; A/B/C=9-282.
DR   InterPro; IPR001369; Mtap_PNP.
DR   InterPro; IPR011268; PNPHPUNA_XAPA.
DR   InterPro; IPR011269; PUNP.
DR   PANTHER; PTHR11904; Mtap_PNP; 1.
DR   Pfam; PF00896; Mtap_PNP; 1.
DR   PIRSF; PIRSF000477; PurNPase; 1.
DR   TIGRFAMs; TIGR01697; PNPH-PUNA-XAPA; 1.
DR   TIGRFAMs; TIGR01698; PUNP; 1.
DR   PROSITE; PS01240; PNP_MTAP_2; 1.
KW   3D-structure; Direct protein sequencing; Glycosyltransferase;
KW   Transferase.
FT   CHAIN         1    282       Purine nucleoside phosphorylase.
FT                                /FTId=PRO_0000184541.
FT   COMPBIAS      1      4       Poly-Thr.
FT   ACT_SITE    204    204       Potential.
FT   TURN         11     12
FT   STRAND       13     13
FT   TURN         14     15
FT   STRAND       16     16
FT   HELIX        18     33
FT   STRAND       36     36
FT   STRAND       39     43
FT   TURN         46     47
FT   TURN         49     54
FT   STRAND       55     55
FT   STRAND       57     63
FT   HELIX        64     66
FT   STRAND       67     67
FT   TURN         68     69
FT   STRAND       81     88
FT   TURN         90     91
FT   STRAND       92     92
FT   STRAND       94     99
FT   STRAND      102    102
FT   HELIX       106    108
FT   STRAND      109    110
FT   HELIX       112    115
FT   STRAND      116    116
FT   HELIX       117    124
FT   TURN        125    126
FT   STRAND      129    138
FT   TURN        141    142
FT   STRAND      143    143
FT   TURN        145    146
FT   STRAND      148    156
FT   STRAND      158    159
FT   STRAND      162    162
FT   STRAND      165    166
FT   TURN        172    173
FT   STRAND      174    175
FT   HELIX       177    186
FT   TURN        188    189
FT   STRAND      192    197
FT   STRAND      201    202
FT   HELIX       206    215
FT   TURN        216    216
FT   STRAND      218    224
FT   HELIX       225    233
FT   TURN        234    235
FT   STRAND      237    247
FT   TURN        249    251
FT   STRAND      253    254
FT   HELIX       258    280
FT   TURN        281    281
SQ   SEQUENCE   282 AA;  29021 MW;  65F468DACC43D360 CRC64;
     TTTTPPSTPP LDDPATDPFL VARAAADHIA QATGVEGHDM ALVLGSGWGG AAELLGEVVA
     EVPTHEIPGF SAPAVAGHLS VTRSIRVERA DGSVRHALVL GSRTHLYEGK GVRAVVHGVR
     TAAATGAETL ILTNGCGGLN QEWGAGTPVL LSDHINLTAR SPLEGPTFVD LTDVYSPRLR
     ELAHRVDPTL PEGVYAQFPG PHYETPAEVR MAGILGADLV GMSTTLEAIA ARHCGLEVLG
     VSLVTNLAAG ISPTPLSHAE VIEAGQAAGP RISALLADIA KR
//