ID   AB194701   standard; genomic DNA; PRO; 1009 BP.
XX
AC   AB194701;
XX
SV   AB194701.1
XX
DT   12-NOV-2004 (Rel. 81, Created)
DT   12-NOV-2004 (Rel. 81, Last updated, Version 1)
XX
DE   Enterobacter cloacae class I integron aadA2 gene for aminoglycoside
DE   adenyltransferase, complete cds.
XX
KW   .
XX
OS   Enterobacter cloacae
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Enterobacter.
XX
RN   [1]
RP   1-1009
RA   Zheng M.P., Shi L., Li L., Cao Y.C., Guo S.Y., Su J.Y., Li X.H., Feng J.;
RT   ;
RL   Submitted (09-NOV-2004) to the EMBL/GenBank/DDBJ databases.
RL   Lei Shi, South China University of Technology, Lab of Molecular Biology,
RL   Food and Biological Engineering; Wushan, Guangzhou, Guangdong, 510640,
RL   China (E-mail:leishi88@hotmail.com, URL:www.scut.edu.cn,
RL   Tel:86-20-87111474, Fax:86-20-87112734)
XX
RN   [2]
RA   Zheng M.P., Shi L., Cao Y.C., Li L., Guo S.Y., Li X.H., Su J.Y., Feng J.;
RT   "Identification of integron-associated resistance gene cassettes from
RT   clinical strains of Enterobacter cloacae";
RL   Unpublished.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1009
FT                   /country="China:Guangzhou"
FT                   /db_xref="taxon:550"
FT                   /mol_type="genomic DNA"
FT                   /organism="Enterobacter cloacae"
FT                   /isolation_source="sputum"
FT                   /strain="GH168"
FT   repeat_region   1..1009
FT                   /transposon="class I integron"
FT   misc_feature    1..97
FT                   /note="class 1 integron 5' conserved region"
FT   misc_feature    98..953
FT                   /note="aadA2 gene cassette"
FT   RBS             107..113
FT                   /gene="aadA2"
FT   CDS             119..898
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /function="resistance to streptomycin and spectinomycin"
FT                   /gene="addA2"
FT                   /product="aminoglycoside adenyltransferase"
FT                   /protein_id="BAD69618.1"
FT                   /translation="MTIEISNQLSEVLSVIERHLESTLLAVHLYGSAVDGGLKPYSDID
FT                   LLVTVAVKLDETTRRALLNDLMEASAFPGESETLRAIEVTLVVHDDIIPWRYPAKRELQ
FT                   FGEWQRNDILAGIFEPAMIDIDLAILLTKAREHSVALVGPAAEEFFDPVPEQDLFEALR
FT                   ETLRLWNSQPDWAGDERNVVLTLSRIWYSAITGKIAPKDVAADWAIKRLPAQYQPVLLE
FT                   AKQAYLGQKEDHLASRADHLEEFIRFVKGEIIKSVGK"
FT   misc_feature    899..953
FT                   /note="aadA2 gene cassette 59-be"
FT   misc_feature    954..1009
FT                   /note="class I integron 3' conserved segment"
XX
SQ   Sequence 1009 BP; 253 A; 251 C; 276 G; 229 T; 0 other;
     ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa        60
     cgatgttacg cagcagggca gtcgccctaa aacaaagtta gacatcatga gggtagcggt       120
     gaccatcgaa atttcgaacc aactatcaga ggtgctaagc gtcattgagc gccatctgga       180
     atcaacgttg ctggccgtgc atttgtacgg ctccgcagtg gatggcggcc tgaagccata       240
     cagcgatatt gatttgttgg ttactgtggc cgtaaagctt gatgaaacga cgcggcgagc       300
     attgctcaat gaccttatgg aggcttcggc tttccctggc gagagcgaga cgctccgcgc       360
     tatagaagtc acccttgtcg tgcatgacga catcatcccg tggcgttatc cggctaagcg       420
     cgagctgcaa tttggagaat ggcagcgcaa tgacattctt gcgggtatct tcgagccagc       480
     catgatcgac attgatctag ctatcctgct tacaaaagca agagaacata gcgttgcctt       540
     ggtaggcccg gcagcggagg aattctttga cccggttcct gaacaggatc tattcgaggc       600
     gctgagggaa accttgaggc tatggaactc gcagcccgac tgggccggcg atgagcgaaa       660
     tgtagtgctt acgttgtccc gcatttggta cagcgcaata accggcaaaa tcgcgccgaa       720
     ggatgtcgct gccgactggg caataaaacg cctacctgcc cagtatcagc ccgtcttact       780
     tgaagctaag caagcttatc tgggacaaaa agaagatcac ttggcctcac gcgcagatca       840
     cttggaagaa tttattcgct ttgtgaaagg cgagatcatc aagtcagttg gtaaatgatg       900
     tctaacaatt cgttcaagcc gaccgcgcta cgcgcggcgg cttaactccg gcgttagatg       960
     cactaagcac ataattgctc acagccaaac tatcaggtca agtctgctt                  1009
//