ID ECAJ2736 standard; genomic DNA; PRO; 2948 BP. XX AC AJ002736; XX SV AJ002736.1 XX DT 06-NOV-1998 (Rel. 57, Created) DT 09-SEP-2004 (Rel. 81, Last updated, Version 3) XX DE Enterobacter cloacae infB gene and partial nusA and rbfA genes XX KW infB gene; initiation factor IF2; nusA gene; rbfA gene; KW ribosome binding factor A. XX OS Enterobacter cloacae OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Enterobacter. XX RN [1] RP 1-2948 RA Sperling-Petersen H.U.; RT ; RL Submitted (05-NOV-1997) to the EMBL/GenBank/DDBJ databases. RL Sperling-Petersen H.U., Department of Molecular and Structural Biology, RL Aarhus University, Gustav Wiedsvej 10C, Aarhus C, DK-8000, DENMARK. XX RN [2] RX MEDLINE; 84193200. RX PUBMED; 6326058. RA Ishii S., Ihara M., Maekawa T., Nakamura Y., Uchida H., Imamoto F.; RT "The nucleotide sequence of the cloned nusA gene and its flanking region of RT Eschericia coli."; RL Nucleic Acids Res. 12(7):3333-3342(1984). XX RN [3] RX MEDLINE; 85088492. RX PUBMED; 6096856. RA Sacerdot C., Dessen P., Hershey J.W., Plumbridge J.A., Grunberg-Manago M.; RT "Sequence of the initiation factor IF2 gene: unusual protein features and RT homologies with elongation factors."; RL Proc. Natl. Acad. Sci. U.S.A. 81(24):7787-7791(1984). XX RN [4] RX MEDLINE; 95212911. RX PUBMED; 7535280. RA Dammel C.S., Noller H.F.; RT "Suppression of a cold-sensitive mutation in 16S rRNA by overexpression of RT a novel ribosome binding factor, RbfA"; RL Genes Dev. 9(5):626-637(1995). XX RN [5] RA Hedegaard J., Steffensen S.A., Norskow-Lauritsen N., Mortensen K.K., RA Sperling-Petersen H.U.; RT "Identification of Enterobacteriaceae by partial sequencing of the gene RT encoding translation initiation factor 2"; RL Int. J. Syst. Bacteriol. 48:1531-1538(1999). XX FH Key Location/Qualifiers FH FT source 1..2948 FT /db_xref="taxon:550" FT /mol_type="genomic DNA" FT /organism="Enterobacter cloacae" FT /strain="EclAU9501" FT CDS <1..75 FT /codon_start=1 FT /db_xref="UniProt/TrEMBL:Q9ZF26" FT /transl_table=11 FT /gene="nusA" FT /product="NusA protein" FT /function="transcription terminator" FT /protein_id="CAA05702.1" FT /translation="GLTDEKAGELIMAARNICWFGDEA" FT RBS 90..94 FT /citation=[2] FT /citation=[3] FT /gene="infB" FT CDS 100..2793 FT /db_xref="GOA:Q9ZF25" FT /db_xref="HSSP:Q9EUZ5" FT /db_xref="UniProt/Swiss-Prot:Q9ZF25" FT /evidence=EXPERIMENTAL FT /transl_table=11 FT /gene="infB" FT /product="initiation factor IF2-alpha" FT /function="translation initiation factor" FT /protein_id="CAA05703.1" FT /translation="MTDVTVKTLAAEIQTSVDRLVQQFADAGIPKSADDSVTAQEKQTL FT LSHLNREHGSAPDKLTLQRKTRSTLNVPGTGGKSKSVQIEVRKTRTFVKRDPQEAERLA FT AEEQAQREAEEQAQREAEITAKREAELKAEREAAEKAKRDASDKAKRDAAEKDKVSNQQ FT TDEMTKTAQTEKARRENEAAELKRKAEEEARRKLEEDARRVAEEARRMAEENEKNGINP FT VEQTEDTSDYHVTTSQHARQAEDENDREVEGGRGRTRTASKTARPQKKGNKHAESKADR FT EEARAAGRGGKGGKRKGSPLLQQGFQKPAQAVNRDVVIGETITVGDLANKMAVKGSQVI FT KAMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP FT VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETDNGMITFLDTPGHAAF FT TSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPLVVAVNKIDKPEADMDRV FT KNELSQYGVMPEEWGGEAQFIPVSAKAGTGIDDLLNAILLQAEVLELKAIRNGMASGAV FT IESFLDKGRGPVATVLVREGTLNKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEI FT LGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVH FT EVNVVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGF FT NVRADASARKVIESESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFK FT SPKFGAIAGCMVTEGNIKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG FT VKNYNDVRVGDMIEVFEIIEIQRTIA" FT CDS 574..2793 FT /db_xref="GOA:Q9ZF25" FT /db_xref="HSSP:Q9EUZ5" FT /db_xref="UniProt/Swiss-Prot:Q9ZF25" FT /evidence=EXPERIMENTAL FT /note="initiation factor IF2-beta results from an in-frame FT tandem translational initiation at codon 159 (GUG) of the FT infB cistron of the messenger RNA" FT /transl_table=11 FT /gene="infB" FT /product="initiation factor IF2-beta" FT /function="translation initiation factor" FT /protein_id="CAA05704.1" FT /translation="MSNQQTDEMTKTAQTEKARRENEAAELKRKAEEEARRKLEEDARR FT VAEEARRMAEENEKNGINPVEQTEDTSDYHVTTSQHARQAEDENDREVEGGRGRTRTAS FT KTARPQKKGNKHAESKADREEARAAGRGGKGGKRKGSPLLQQGFQKPAQAVNRDVVIGE FT TITVGDLANKMAVKGSQVIKAMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENEL FT EEAVMSDRDTGAAAEPRAPVVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYH FT VETDNGMITFLDTPGHAAFTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQV FT PLVVAVNKIDKPEADMDRVKNELSQYGVMPEEWGGEAQFIPVSAKAGTGIDDLLNAILL FT QAEVLELKAIRNGMASGAVIESFLDKGRGPVATVLVREGTLNKGDIVLCGFEYGRVRAM FT RNELGQEVLEAGPSIPVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLAR FT QQKSKLENMFANMTEGEVHEVNVVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGG FT ITETDATLAAASNAILVGFNVRADASARKVIESESLDLRYYSVIYNLIDEVKAAMSGML FT SPELKQQIIGLAEVRDVFKSPKFGAIAGCMVTEGNIKRHNPIRVLRDNVVIYEGELESL FT RRFKDDVNEVRNGMECGIGVKNYNDVRVGDMIEVFEIIEIQRTIA" FT CDS 598..2793 FT /db_xref="GOA:Q9ZF25" FT /db_xref="HSSP:Q9EUZ5" FT /db_xref="UniProt/Swiss-Prot:Q9ZF25" FT /note="initiation factor IF2-gamma is predicted to be FT translated from an in-frame tandem translational initiation FT at codon 167 (AUG) of the infB cistron of the messenger FT RNA." FT /transl_table=11 FT /gene="infB" FT /product="initiation factor IF2-gamma" FT /function="translation initiation factor" FT /protein_id="CAA05705.1" FT /translation="MTKTAQTEKARRENEAAELKRKAEEEARRKLEEDARRVAEEARRM FT AEENEKNGINPVEQTEDTSDYHVTTSQHARQAEDENDREVEGGRGRTRTASKTARPQKK FT GNKHAESKADREEARAAGRGGKGGKRKGSPLLQQGFQKPAQAVNRDVVIGETITVGDLA FT NKMAVKGSQVIKAMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDR FT DTGAAAEPRAPVVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETDNGMI FT TFLDTPGHAAFTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPLVVAVNK FT IDKPEADMDRVKNELSQYGVMPEEWGGEAQFIPVSAKAGTGIDDLLNAILLQAEVLELK FT AIRNGMASGAVIESFLDKGRGPVATVLVREGTLNKGDIVLCGFEYGRVRAMRNELGQEV FT LEAGPSIPVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLEN FT MFANMTEGEVHEVNVVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATL FT AAASNAILVGFNVRADASARKVIESESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQI FT IGLAEVRDVFKSPKFGAIAGCMVTEGNIKRHNPIRVLRDNVVIYEGELESLRRFKDDVN FT EVRNGMECGIGVKNYNDVRVGDMIEVFEIIEIQRTIA" FT terminator 2842..2865 FT /citation=[2] FT /citation=[3] FT /gene="infB" FT RBS 2871..2874 FT /citation=[2] FT /citation=[3] FT /gene="rbfA" FT CDS 2883..>2948 FT /db_xref="GOA:Q9ZF24" FT /db_xref="HSSP:P09170" FT /db_xref="UniProt/TrEMBL:Q9ZF24" FT /transl_table=11 FT /gene="rbfA" FT /product="ribosome binding factor A" FT /function="ribosome maturation" FT /protein_id="CAA05706.1" FT /translation="MAKEFGRPQRVAQEMQKEIALI" XX SQ Sequence 2948 BP; 796 A; 722 C; 848 G; 582 T; 0 other; ggtttaaccg acgagaaagc cggcgaactc atcatggccg cacgtaatat ttgctggttc 60 ggcgacgaag cgtaataaac tgtagcagga aggaacagca tgactgatgt aactgtaaaa 120 acgctggctg ctgagattca gacttccgtg gaccgcctgg tacagcaatt tgctgatgca 180 gggatcccga agtccgctga tgactcagtg accgcgcaag agaaacaaac cttattatcg 240 cacctgaacc gcgaacacgg ttcggcgcct gataaactga cgttacagcg taaaacgcgc 300 agtacgttaa atgttccagg taccggtggc aaaagcaaat cggtacaaat cgaagtccgc 360 aagacacgca cctttgtaaa acgtgatccg caagaggcag aacgccttgc cgcggaagag 420 caggcacagc gtgaagcgga agaacaagct cagcgtgagg cagaaattac tgccaaacgt 480 gaggcagaat taaaagctga acgtgaggcc gcagaaaaag cgaagcgcga tgccagtgat 540 aaagcgaagc gtgacgcggc ggaaaaagac aaagtgagca atcaacagac tgacgaaatg 600 accaaaacag cccagacgga aaaagcccgt cgtgaaaatg aagcggccga actgaagcgt 660 aaagcggaag aagaagctcg ccgtaagctg gaagaagacg cacgtcgtgt tgctgaagaa 720 gcgcgccgta tggcagaaga aaacgagaag aacggtatca atcctgtcga gcagacagaa 780 gacaccagtg attatcacgt aaccacttct cagcacgcac gccaggcaga agacgaaaac 840 gaccgcgaag ttgagggtgg ccgtggccgt acccgcactg cgtcgaaaac tgcccgtcct 900 cagaagaaag gcaataagca cgctgaatcc aaagccgatc gcgaagaagc gcgtgcagcg 960 ggtcgcggtg gtaaaggcgg taagcgtaaa ggttcgccgc tgctgcagca gggcttccag 1020 aagccagctc aggccgttaa ccgtgacgtt gtgattggcg aaaccatcac cgttggcgat 1080 ctggcgaaca agatggcggt taaaggctct caggtcatca aagcgatgat gaagctgggc 1140 gcaatggcca ccatcaacca ggttatcgat caggaaaccg cacagctggt tgccgaagag 1200 atgggccaca aagttatcct gcgtcgtgaa aacgagctgg aagaagcagt aatgagcgac 1260 cgtgacactg gcgcagcggc tgaaccgcgt gcaccggttg tgaccatcat gggccacgtt 1320 gaccacggta aaacctctct gctcgactac attcgttcta ctaaggttgc ctccggcgaa 1380 gcgggtggta ttacccagca catcggtgct taccacgtag aaaccgataa cggcatgatc 1440 accttcctgg ataccccagg ccacgccgcg tttacctcaa tgcgtgctcg tggtgctcag 1500 gcaacggata tcgttgtgct ggttgttgct gctgacgatg gcgtgatgcc acagaccatt 1560 gaagctatcc agcacgcgaa agcggcgcag gtgcctctgg ttgttgcagt taacaaaatc 1620 gataagccag aagccgatat ggaccgcgtt aagaacgaac tgtctcagta cggcgttatg 1680 ccggaagagt ggggcggcga agcgcagttc atcccggtat ctgctaaagc aggtaccggt 1740 atcgacgacc tgctgaacgc aatcctgctg caggctgaag tacttgagct gaaagcgatt 1800 cgtaacggta tggcgagcgg tgcggtcatc gaatccttcc tggataaagg tcgtggtccg 1860 gttgcaaccg ttctggttcg cgaaggtacg ctgaacaagg gcgacatcgt tctttgcggc 1920 ttcgaatacg gtcgcgttcg tgcgatgcgt aacgaactgg gtcaggaagt gctggaagca 1980 ggtccatcca ttccagtgga aatcctgggt ctgtccggtg ttccggctgc cggtgacgaa 2040 gtgaccgttg tgcgtgacga gaagaaagcg cgtgaagttg cactgtatcg tcagggcaaa 2100 ttccgtgaag ttaagctggc tcgtcagcag aaatctaaac tcgagaacat gtttgcgaac 2160 atgaccgaag gcgaagttca cgaagtgaac gtcgtactga aagccgacgt tcagggttct 2220 gtggaagcga tctccgactc cttgctgaaa ctgtctactg acgaagttaa agtgaagatc 2280 atcggttctg gcgtaggtgg tatcaccgaa accgacgcaa ccctggctgc agcctcaaac 2340 gctatcctgg ttggcttcaa cgttcgtgcg gatgcgtctg cgcgtaaagt gattgaatcc 2400 gaaagcctgg atctgcgcta ctactccgtc atctataacc tgatcgacga agtgaaagca 2460 gcgatgagcg gcatgctgtc tcctgagctg aaacagcaga ttatcggtct ggctgaagta 2520 cgtgacgtgt tcaaatcacc taaattcggt gcgatcgcgg gctgtatggt taccgaaggt 2580 aacatcaagc gtcacaaccc aatccgcgta ctgcgtgaca acgtggttat ctacgaaggc 2640 gagctggaat ccctgcgccg cttcaaagat gacgttaacg aagtccgtaa cggcatggaa 2700 tgtggtatcg gcgttaagaa ctacaacgac gttcgcgttg gcgatatgat cgaagtgttc 2760 gagatcatcg aaattcagcg tactatcgct taattaccga ttgtaagccg ggatcgcctc 2820 gcgccacccg gcaataattt taaaaagggg ctttagcccc ttttttgtct ggagaattta 2880 ttatggcgaa agaatttggt cgccctcagc gcgtagcgca ggaaatgcag aaggagatcg 2940 cgctcatt 2948 //