ID MTEC_ENTCL STANDARD; PRT; 452 AA. AC P14827; DT 01-APR-1990 (Rel. 14, Created) DT 01-APR-1990 (Rel. 14, Last sequence update) DT 25-OCT-2004 (Rel. 45, Last annotation update) DE Modification methylase EcaI (EC 2.1.1.72) (Adenine-specific DE methyltransferase EcaI) (M.EcaI). GN Name=ecaIM; OS Enterobacter cloacae. OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Enterobacter. OX NCBI_TaxID=550; RN [1] RP NUCLEOTIDE SEQUENCE. RC STRAIN=DSM 30056; RX MEDLINE=90221818; PubMed=2183182; RA Brenner V., Venetianer P., Kiss A.; RT "Cloning and nucleotide sequence of the gene encoding the EcaI DNA RT methyltransferase."; RL Nucleic Acids Res. 18:355-359(1990). CC -!- FUNCTION: This methylase recognizes the double-stranded sequence CC GGTNACC, causes specific methylation on A-5 on both strands and CC protects the DNA from cleavage by the EcaI endonuclease. CC -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + DNA adenine = S- CC adenosyl-L-homocysteine + DNA 6-methylaminopurine. CC -!- SIMILARITY: Belongs to the N4/N6-methyltransferase family. CC -------------------------------------------------------------------------- CC This Swiss-Prot entry is copyright. It is produced through a collaboration CC between the Swiss Institute of Bioinformatics and the EMBL outstation - CC the European Bioinformatics Institute. There are no restrictions on its CC use as long as its content is in no way modified and this statement is not CC removed. CC -------------------------------------------------------------------------- DR EMBL; X17111; CAA34968.1; -. DR PIR; S07886; S07886. DR HSSP; P23192; 1G60. DR REBASE; 3365; M.EcaI. DR GO; GO:0005578; C:extracellular matrix (sensu Metazoa); ISS. DR GO; GO:0008083; F:growth factor activity; ISS. DR GO; GO:0007267; P:cell-cell signaling; ISS. DR GO; GO:0030595; P:immune cell chemotaxis; ISS. DR GO; GO:0050930; P:induction of positive chemotaxis; ISS. DR GO; GO:0042102; P:positive regulation of T-cell proliferation; ISS. DR GO; GO:0045637; P:regulation of myeloid blood cell differenti...; ISS. DR GO; GO:0042088; P:T-helper 1 type immune response; ISS. DR InterPro; IPR002295; D21N6_mtfrase. DR InterPro; IPR001091; Met_trans_CN4. DR InterPro; IPR002941; N6/N4_Mtase. DR InterPro; IPR002052; N6_Mtase. DR Pfam; PF01555; N6_N4_Mtase; 1. DR PRINTS; PR00506; D21N6MTFRASE. DR PRINTS; PR00508; S21N4MTFRASE. DR PROSITE; PS00092; N6_MTASE; 1. KW Methyltransferase; Restriction system; Transferase. SQ SEQUENCE 452 AA; 51112 MW; C19D9582E4AE9535 CRC64; MAVGLNKKEI DGPASQRAVA CDLEPALPPI GARVTLNYPG KMDESIILQK KDTKYLRVGS DFAKESSLIL PNSFIWSDNS LALNRLMVEG KKAKLIYLDP PYATGMGFSS RSNEHAYDDC LTEAAYLEFM RRRLILMREI LDDDGTIYVH IGHQMLGELK CLLDEIFGRE RFINLITRRK CSSKNSTKNN FANLNDYILC YSKGKKYIWN RPLKKPDAEW LAKEYPKTDS KGQFKLVPIH APGVRHGATG GEWKGMLPPP GKHWQYTPEK LDILDASGDI HWSKTGNPRR KVYLTDDKSI GYTDYWEEFR DAHHQSILVT GYPTEKNFNM MKLIVGASSN PGDLVIDPFC GSGSTLHAAS LLQRKWIGID ESLFAAKTVM KRFAIGRAPM GDYVNTSLNK QTELPLSLNE TARHEYVSND FNIYVDELTA SVSKNELAEI QKAYRDLKAN QQ //