ID   BLA1_ENTCL     STANDARD;      PRT;   286 AA.
AC   P18251;
DT   01-NOV-1990 (Rel. 16, Created)
DT   01-NOV-1990 (Rel. 16, Last sequence update)
DT   01-FEB-2005 (Rel. 46, Last annotation update)
DE   Beta-lactamase Ohio-1 precursor (EC 3.5.2.6).
OS   Enterobacter cloacae.
OG   Plasmid pDS075.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Enterobacter.
OX   NCBI_TaxID=550;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   MEDLINE=91024125; PubMed=2121093;
RA   Shlaes D.M., Currie-Mccumber C., Hull A., Behlau I., Kron M.;
RT   "OHIO-1 beta-lactamase is part of the SHV-1 family.";
RL   Antimicrob. Agents Chemother. 34:1570-1576(1990).
CC   -!- CATALYTIC ACTIVITY: A beta-lactam + H(2)O = a substituted beta-
CC       amino acid.
CC   -!- SIMILARITY: Belongs to the class-A beta-lactamase family.
CC   --------------------------------------------------------------------------
CC   This Swiss-Prot entry is copyright. It is produced through a collaboration
CC   between  the Swiss Institute of Bioinformatics  and the  EMBL outstation -
CC   the European Bioinformatics Institute.  There are no  restrictions on  its
CC   use as long as its content is in no way modified and this statement is not
CC   removed.
CC   --------------------------------------------------------------------------
DR   EMBL; M33655; AAA72078.1; -.
DR   PIR; A44958; A44958.
DR   HSSP; P30896; 1N9B.
DR   InterPro; IPR001466; Beta_lactamase.
DR   InterPro; IPR000871; Beta_lactamase_A.
DR   Pfam; PF00144; Beta-lactamase; 1.
DR   PRINTS; PR00118; BLACTAMASEA.
DR   PROSITE; PS00146; BETA_LACTAMASE_A; 1.
KW   Antibiotic resistance; Hydrolase; Plasmid; Signal.
FT   SIGNAL        1     21
FT   CHAIN        22    286       Beta-lactamase Ohio-1.
FT   ACT_SITE     66     66       Acyl-ester intermediate (By similarity).
FT   ACT_SITE    164    164       Proton acceptor (By similarity).
FT   SITE        230    232       Substrate binding (By similarity).
FT   DISULFID     73    119       By similarity.
SQ   SEQUENCE   286 AA;  31354 MW;  9FE16709361AF9C5 CRC64;
     MRYFRLCIIS LLATLPLRVH AGPQPLEQIK LSESQLSGSV GMIEMDLARP GTLTAWRADE
     RFPMMSTFKV VLCGAGLARV DAGDEQLERK IHYRRQDLVD YSPVSEKHLA DGMTVGELCA
     AAITMSDNSA ANLLLPAVGG PAGLTAFLRQ IGDNVTRLDR WETELNEALP GDARDTTTAR
     SMAATLRKLL TSQRLSARSQ RQLLQWMVDD RVAGRLIRSV LPAGWFIADK TGAGERGARG
     IVALLGPNNK AERIVVIYLR DTPASMAERN QQIAGIAGAL IEHWQR
//