ID   LRP_ECOLI      STANDARD;      PRT;   163 AA.
AC   P19494;
DT   01-FEB-1991 (Rel. 17, Created)
DT   15-DEC-1998 (Rel. 37, Last sequence update)
DT   01-FEB-2005 (Rel. 46, Last annotation update)
DE   Leucine-responsive regulatory protein.
GN   Name=lrp; Synonyms=alsB, ihb, livR, oppI;
GN   OrderedLocusNames=b0889, c1026, z1234, ECs0974, SF0848, S0889;
OS   Escherichia coli,
OS   Escherichia coli O6,
OS   Escherichia coli O157:H7,
OS   Shigella flexneri, and
OS   Enterobacter aerogenes (Aerobacter aerogenes).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562, 217992, 83334, 623, 548;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   SPECIES=E.coli;
RX   MEDLINE=91250369; PubMed=2040596;
RA   Willins D.A., Ryan C., Platko J.V., Calvo J.M.;
RT   "Characterization of Lrp, and Escherichia coli regulatory protein that
RT   mediates a global response to leucine.";
RL   J. Biol. Chem. 266:10768-10774(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RC   SPECIES=E.coli;
RA   Ito K., Kawakami K., Nakamura Y.;
RL   Submitted (MAY-1992) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   SPECIES=E.coli; STRAIN=K12 / MG1655;
RX   MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA   Mau B., Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1474(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE.
RC   SPECIES=E.coli; STRAIN=K12;
RX   MEDLINE=97061202; PubMed=8905232;
RA   Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A.,
RA   Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K.,
RA   Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K.,
RA   Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N.,
RA   Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y.,
RA   Yano M., Horiuchi T.;
RT   "A 718-kb DNA sequence of the Escherichia coli K-12 genome
RT   corresponding to the 12.7-28.0 min region on the linkage map.";
RL   DNA Res. 3:137-155(1996).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   SPECIES=E.coli; STRAIN=O6:H1 / CFT073 / ATCC 700928 / UPEC;
RX   MEDLINE=22388234; PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P.,
RA   Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D.,
RA   Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T.,
RA   Mobley H.L.T., Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence
RT   of uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   SPECIES=E.coli; STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   MEDLINE=21074935; PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D.,
RA   Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A.,
RA   Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L.,
RA   Grotbeck E.J., Davis N.W., Lim A., Dimalanta E.T., Potamousis K.,
RA   Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C.,
RA   Welch R.A., Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   SPECIES=E.coli; STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   MEDLINE=21156231; PubMed=11258796;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T.,
RA   Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.,
RA   Kuhara S., Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli
RT   O157:H7 and genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
RN   [8]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   SPECIES=S.flexneri; STRAIN=301 / Serotype 2a;
RX   MEDLINE=22272406; PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H.,
RA   Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J.,
RA   Sun L., Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S.,
RA   Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y.,
RA   Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [9]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   SPECIES=S.flexneri; STRAIN=2457T / ATCC 700930 / Serotype 2a;
RX   MEDLINE=22590274; PubMed=12704152;
RX   DOI=10.1128/IAI.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W.,
RA   Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A.,
RA   Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S.,
RA   Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella
RT   flexneri serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
RN   [10]
RP   NUCLEOTIDE SEQUENCE.
RC   SPECIES=E.aerogenes;
RX   MEDLINE=95189743; PubMed=7883720;
RA   Friedberg D., Platko J.V., Tyler B., Calvo J.M.;
RT   "The amino acid sequence of Lrp is highly conserved in four enteric
RT   microorganisms.";
RL   J. Bacteriol. 177:1624-1626(1995).
RN   [11]
RP   PARTIAL PROTEIN SEQUENCE OF N-TERMINUS.
RC   SPECIES=E.coli;
RX   MEDLINE=90330567; PubMed=2115869;
RA   Platko J.V., Willins D.A., Calvo J.M.;
RT   "The ilvIH operon of Escherichia coli is positively regulated.";
RL   J. Bacteriol. 172:4563-4570(1990).
RN   [12]
RP   CHARACTERIZATION.
RC   SPECIES=E.coli;
RX   MEDLINE=92104949; PubMed=1729203;
RA   Haney S.A., Platko J.V., Oxender D.L., Calvo J.M.;
RT   "Lrp, a leucine-responsive protein, regulates branched-chain amino
RT   acid transport genes in Escherichia coli.";
RL   J. Bacteriol. 174:108-115(1992).
RN   [13]
RP   REVIEW.
RC   SPECIES=E.coli;
RX   MEDLINE=94025042; PubMed=8212136; DOI=10.1016/0968-0004(93)90177-O;
RA   D'Ari R., Lin R.T., Newman E.B.;
RT   "The leucine-responsive regulatory protein: more than a regulator?";
RL   Trends Biochem. Sci. 18:260-263(1993).
CC   -!- FUNCTION: Mediates a global response to leucine. Exogenous leucine
CC       affects the expression of a number of different operons; lrp
CC       mediates this effect for at least some of these operons. For
CC       example it is regulator of the branched-chain amino acid transport
CC       genes.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Contains 1 HTH asnC-type DNA-binding domain.
CC   --------------------------------------------------------------------------
CC   This Swiss-Prot entry is copyright. It is produced through a collaboration
CC   between  the Swiss Institute of Bioinformatics  and the  EMBL outstation -
CC   the European Bioinformatics Institute.  There are no  restrictions on  its
CC   use as long as its content is in no way modified and this statement is not
CC   removed.
CC   --------------------------------------------------------------------------
DR   EMBL; M35869; AAA24089.1; -.
DR   EMBL; D11105; BAA01880.1; -.
DR   EMBL; U00096; AAC73975.1; -.
DR   EMBL; D90726; BAA35614.1; -.
DR   EMBL; D90727; BAA35621.1; -.
DR   EMBL; AE016758; AAN79498.1; ALT_INIT.
DR   EMBL; AE005278; AAG55376.1; -.
DR   EMBL; AP002553; BAB34397.1; -.
DR   EMBL; AE015113; AAN42481.1; ALT_INIT.
DR   EMBL; AE016981; AAP16353.1; -.
DR   EMBL; U02272; AAA75439.1; -.
DR   PIR; D85614; D85614.
DR   PIR; F90750; F90750.
DR   PIR; JH0412; RGECLR.
DR   ECO2DBASE; I015.1; 6TH EDITION.
DR   EchoBASE; EB0542; -.
DR   EcoGene; EG10547; lrp.
DR   InterPro; IPR011008; Dimer_A_B_barrel.
DR   InterPro; IPR000485; HTH_AsnC_lrp.
DR   InterPro; IPR009058; Wing_hlx_DNA_bnd.
DR   Pfam; PF01037; AsnC_trans_reg; 1.
DR   PRINTS; PR00033; HTHASNC.
DR   SMART; SM00344; HTH_ASNC; 1.
DR   PROSITE; PS00519; HTH_ASNC_1; 1.
DR   PROSITE; PS50956; HTH_ASNC_2; 1.
KW   Activator; Complete proteome; Direct protein sequencing; DNA-binding;
KW   Transcription regulation.
FT   INIT_MET      0      0
FT   DOMAIN       11     72       HTH asnC-type.
FT   DNA_BIND     30     49       H-T-H motif (Potential).
FT   VARIANT     113    113       D -> E (in lrp-1 mutant).
SQ   SEQUENCE   163 AA;  18756 MW;  B0083495B8F7B255 CRC64;
     VDSKKRPGKD LDRIDRNILN ELQKDGRISN VELSKRVGLS PTPCLERVRR LERQGFIQGY
     TALLNPHYLD ASLLVFVEIT LNRGAPDVFE QFNTAVQKLE EIQECHLVSG DFDYLLKTRV
     PDMSAYRKLL GETLLRLPGV NDTRTYVVME EVKQSNRLVI KTR
//