ID   G3P_ENTAE      STANDARD;      PRT;   294 AA.
AC   P24163;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 1.
DT   04-APR-2006, entry version 44.
DE   Glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) (GAPDH)
DE   (Fragment).
GN   Name=gap;
OS   Enterobacter aerogenes (Aerobacter aerogenes).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Enterobacter.
OX   NCBI_TaxID=548;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=E482;
RX   MEDLINE=92065252; PubMed=1955870;
RA   Lawrence J.G., Ochman H., Hartl D.L.;
RT   "Molecular and evolutionary relationships among enteric bacteria.";
RL   J. Gen. Microbiol. 137:1911-1921(1991).
CC   -!- CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate +
CC       NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from
CC       D-glyceraldehyde 3-phosphate: step 1.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the glyceraldehyde-3-phosphate
CC       dehydrogenase family.
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DR   EMBL; M63372; AAA24801.1; -; Genomic_DNA.
DR   HSSP; P06977; 1DC5.
DR   SMR; P24163; 1-294.
DR   InterPro; IPR000173; GAP_DH.
DR   InterPro; IPR006424; GAPDH_I.
DR   PANTHER; PTHR10836; GAP_dhdrogenase; 1.
DR   Pfam; PF02800; Gp_dh_C; 1.
DR   Pfam; PF00044; Gp_dh_N; 1.
DR   PRINTS; PR00078; G3PDHDRGNASE.
DR   TIGRFAMs; TIGR01534; GAPDH-I; 1.
DR   PROSITE; PS00071; GAPDH; FALSE_NEG.
KW   Glycolysis; NAD; Oxidoreductase.
FT   CHAIN        <1   >294       Glyceraldehyde-3-phosphate dehydrogenase.
FT                                /FTId=PRO_0000145660.
FT   REGION      134    136       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   REGION      194    195       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   ACT_SITE    135    135       Nucleophile (By similarity).
FT   BINDING      19     19       NAD (By similarity).
FT   BINDING      63     63       NAD; via carbonyl oxygen (By similarity).
FT   BINDING     165    165       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     217    217       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   SITE        162    162       Activates thiol group during catalysis
FT                                (By similarity).
FT   NON_TER       1      1
FT   NON_TER     294    294
SQ   SEQUENCE   294 AA;  31448 MW;  F22CA5172621CD8D CRC64;
     IVFRAAQKRS DIEIVGINDL LDAEYMAYML KYDSTHGRFD GTVEVKDGHL VVNGKTIRVT
     AEKDPANLKW NEIGVDVVAE ATGIFLTDET ARKHITAGAK KVVLTGPSKD NTPMFVRGAN
     FETYAGQDIV SNRSCTTNCL APLAKVINDN FGIIEGLMTT VHATTATQKT VDGPSHKDWR
     GGRGAAQNII PSSTGAAKAV GKVLPELNGK LTGMAFRVPT PNVSVVDLTV RLEKAASYEE
     IKKAIKAASE GPMKGVLGYT EDDVVSTDFN GEVCTSVFDA KAGIALNDNF VKLV
//