ID   BLAN_ENTCL     STANDARD;      PRT;   292 AA.
AC   P52663;
DT   01-OCT-1996 (Rel. 34, Created)
DT   01-OCT-1996 (Rel. 34, Last sequence update)
DT   01-FEB-2005 (Rel. 46, Last annotation update)
DE   Imipenem-hydrolyzing beta-lactamase precursor (EC 3.5.2.6)
DE   (Carbapenemase) (NMC-A).
GN   Name=nmcA;
OS   Enterobacter cloacae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Enterobacter.
OX   NCBI_TaxID=550;
RN   [1]
RP   NUCLEOTIDE SEQUENCE, AND PROTEIN SEQUENCE OF 28-34.
RC   STRAIN=NOR-1;
RX   MEDLINE=94329582; PubMed=8052644;
RA   Naas T., Nordmann P.;
RT   "Analysis of a carbapenem-hydrolyzing class A beta-lactamase from
RT   Enterobacter cloacae and of its LysR-type regulatory protein.";
RL   Proc. Natl. Acad. Sci. U.S.A. 91:7693-7697(1994).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (1.89 ANGSTROMS).
RC   STRAIN=NOR-1;
RX   MEDLINE=99395084; PubMed=10464248; DOI=10.1074/jbc.274.36.25260;
RA   Mourey L., Kotra L.P., Bellettini J., Bulychev A., O'Brien M.,
RA   Miller M.J., Mobashery S., Samama J.-P.;
RT   "Inhibition of the broad spectrum nonmetallocarbapenamase of class A
RT   (NMC-A) beta-lactamase from Enterobacter cloacae by monocyclic beta-
RT   lactams.";
RL   J. Biol. Chem. 274:25260-25265(1999).
CC   -!- FUNCTION: Hydrolyzes carbapenems such as imipenem, which are
CC       extended-spectrum beta-lactam antibiotics.
CC   -!- CATALYTIC ACTIVITY: A beta-lactam + H(2)O = a substituted beta-
CC       amino acid.
CC   -!- SIMILARITY: Belongs to the class-A beta-lactamase family.
CC   --------------------------------------------------------------------------
CC   This Swiss-Prot entry is copyright. It is produced through a collaboration
CC   between  the Swiss Institute of Bioinformatics  and the  EMBL outstation -
CC   the European Bioinformatics Institute.  There are no  restrictions on  its
CC   use as long as its content is in no way modified and this statement is not
CC   removed.
CC   --------------------------------------------------------------------------
DR   EMBL; Z21956; CAA79967.1; -.
DR   PIR; S35915; S35915.
DR   PDB; 1BUE; X-ray; A=28-292.
DR   PDB; 1BUL; X-ray; @=28-292.
DR   InterPro; IPR001466; Beta_lactamase.
DR   InterPro; IPR000871; Beta_lactamase_A.
DR   Pfam; PF00144; Beta-lactamase; 1.
DR   PRINTS; PR00118; BLACTAMASEA.
DR   PROSITE; PS00146; BETA_LACTAMASE_A; FALSE_NEG.
KW   3D-structure; Antibiotic resistance; Direct protein sequencing;
KW   Hydrolase; Signal.
FT   SIGNAL        1     27
FT   CHAIN        28    292       Imipenem-hydrolyzing beta-lactamase.
FT   ACT_SITE     71     71       Acyl-ester intermediate.
FT   SITE        236    238       Substrate binding (By similarity).
FT   DISULFID     70    240
FT   TURN         30     31
FT   HELIX        32     42
FT   TURN         43     43
FT   STRAND       45     52
FT   TURN         53     55
FT   STRAND       58     61
FT   TURN         63     64
FT   STRAND       67     68
FT   HELIX        70     73
FT   HELIX        74     86
FT   TURN         87     88
FT   TURN         92     93
FT   STRAND       95     96
FT   TURN         99    100
FT   HELIX       108    112
FT   TURN        113    116
FT   STRAND      118    119
FT   HELIX       120    129
FT   HELIX       133    142
FT   TURN        143    144
FT   HELIX       146    156
FT   TURN        157    158
FT   STRAND      163    163
FT   TURN        168    169
FT   HELIX       170    172
FT   TURN        176    177
FT   TURN        180    181
FT   STRAND      182    183
FT   HELIX       185    197
FT   HELIX       203    214
FT   TURN        215    215
FT   TURN        220    222
FT   HELIX       223    226
FT   TURN        229    230
FT   STRAND      232    239
FT   TURN        243    244
FT   STRAND      246    253
FT   TURN        255    256
FT   STRAND      260    267
FT   TURN        271    272
FT   HELIX       277    289
FT   TURN        290    290
SQ   SEQUENCE   292 AA;  32075 MW;  4D33FD8669998F1B CRC64;
     MSLNVKQSRI AILFSSCLIS ISFFSQANTK GIDEIKNLET DFNGRIGVYA LDTGSGKSFS
     YRANERFPLC SSFKGFLAAA VLKGSQDNRL NLNQIVNYNT RSLEFHSPIT TKYKDNGMSL
     GDMAAAALQY SDNGATNIIL ERYIGGPEGM TKFMRSIGDE DFRLDRWELD LNTAIPGDER
     DTSTPAAVAK SLKTLALGNI LSEHEKETYQ TWLKGNTTGA ARIRASVPSD WVVGDKTGSC
     GAYGTANDYA VVWPKNRAPL IISVYTTKNE KEAKHEDKVI AEASRIAIDN LK
//