ID   1A1D_ENTCL     STANDARD;      PRT;   338 AA.
AC   Q9ZHW3; Q9ZHW4;
DT   30-MAY-2000 (Rel. 39, Created)
DT   30-MAY-2000 (Rel. 39, Last sequence update)
DT   01-FEB-2005 (Rel. 46, Last annotation update)
DE   1-aminocyclopropane-1-carboxylate deaminase (EC 3.5.99.7) (ACC
DE   deaminase).
OS   Enterobacter cloacae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Enterobacter.
OX   NCBI_TaxID=550;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=CAL2, and UW4;
RX   MEDLINE=99068011; PubMed=9851025;
RA   Shah S., Li J., Moffatt B.A., Glick B.R.;
RT   "Isolation and characterization of ACC deaminase genes from two
RT   different plant growth-promoting rhizobacteria.";
RL   Can. J. Microbiol. 44:833-843(1998).
CC   -!- FUNCTION: Catalyzes the conversion of 1-aminocyclopropane-1-
CC       carboxylate (ACC), the precursor to the phytohormone ethylene, to
CC       ammonia and alpha-ketobutyrate. Allows growth on ACC as a nitrogen
CC       source.
CC   -!- CATALYTIC ACTIVITY: 1-aminocyclopropane-1-carboxylate + H(2)O = 2-
CC       oxobutanoate + NH(3).
CC   -!- COFACTOR: Pyridoxal phosphate.
CC   -!- SIMILARITY: Belongs to the ACC deaminase/D-cysteine desulfhydrase
CC       family.
CC   --------------------------------------------------------------------------
CC   This Swiss-Prot entry is copyright. It is produced through a collaboration
CC   between  the Swiss Institute of Bioinformatics  and the  EMBL outstation -
CC   the European Bioinformatics Institute.  There are no  restrictions on  its
CC   use as long as its content is in no way modified and this statement is not
CC   removed.
CC   --------------------------------------------------------------------------
DR   EMBL; AF047840; AAD05070.1; -.
DR   EMBL; AF047710; AAD05069.1; -.
DR   HSSP; O57809; 1J0A.
DR   InterPro; IPR005965; Amcycprop_deam.
DR   InterPro; IPR001926; B6_enzyme_beta.
DR   Pfam; PF00291; PALP; 1.
DR   TIGRFAMs; TIGR01274; ACC_deam; 1.
KW   Hydrolase; Pyridoxal phosphate.
FT   BINDING      51     51       Pyridoxal phosphate (covalent) (By
FT                                similarity).
FT   VARIANT      26     26       Q -> E (in strain UW4).
FT   VARIANT     151    151       Q -> R (in strain UW4).
FT   VARIANT     178    178       K -> E (in strain UW4).
FT   VARIANT     185    185       E -> D (in strain UW4).
FT   VARIANT     191    191       N -> D (in strain UW4).
FT   VARIANT     313    313       E -> D (in strain UW4).
FT   VARIANT     337    337       D -> N (in strain UW4).
SQ   SEQUENCE   338 AA;  36871 MW;  0B94C8C887772AF8 CRC64;
     MNLNRFERYP LTFGPSPITP LKRLSQHLGG KVELYAKRED CNSGLAFGGN KTRKLEYLIP
     EAIEQGCDTL VSIGGIQSNQ TRQVAAVAAH LGMKCVLVQE NWVNYSDAVY DRVGNIEMSR
     IMGADVRLDA AGFDIGIRPS WEKAMSDVVE QGGKPFPIPA GCSEHPYGGL GFVGFAKKLR
     QQEKELGFKF NYIVVCSVTG STQAGMVVGF AADGRSKNVI GVDASAKPEQ TKAQILRIAR
     HTAELVELGR EITEEDVVLD TRFAYPEYGL PNEGTLEAIR LCGSLEGVLT DPVYEGKSMH
     GMIEMVRRGE FPEGSKVLYA HLGGAPALNA YSFLFRDG
//