ID AB196476 standard; genomic DNA; PRO; 2855 BP. XX AC AB196476; XX SV AB196476.1 XX DT 14-DEC-2004 (Rel. 82, Created) DT 14-DEC-2004 (Rel. 82, Last updated, Version 1) XX DE Herbaspirillum sp. B501 nifH, nifD genes for nitrogenase iron protein, DE nitrogenase Mo-Fe protein alpha chain, complete and partial cds. XX KW . XX OS Herbaspirillum sp. B501 OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; OC Oxalobacteraceae; Herbaspirillum. XX RN [1] RP 1-2855 RA Minamisawa K.; RT ; RL Submitted (10-DEC-2004) to the EMBL/GenBank/DDBJ databases. RL Kiwamu Minamisawa, Tohoku University, Graduate School of Life Sciences; RL Katahira, Aoba-ku, Sendai, Miyagi, 980-8577, Japan RL (E-mail:kiwamu@ige.tohoku.ac.jp, Tel:81-22-217-5684, Fax:81-22-263-9845) XX RN [2] RA You M., Iwasa T., Mitsui H., Minamisawa K.; RT "Expression analysis of nifH gene in free-living and endophytic RT Herbaspirillum spiecies by reverse transcription-PCR"; RL Unpublished. XX FH Key Location/Qualifiers FH FT source 1..2855 FT /db_xref="taxon:137773" FT /mol_type="genomic DNA" FT /organism="Herbaspirillum sp. B501" FT /specific_host="Oryza officinalis W0012" FT /strain="B501" FT misc_binding 342..357 FT /bound_moiety="NifA" FT /function="potential NifA upstream activator sequence" FT misc_binding 372..387 FT /bound_moiety="NifA" FT /function="potential NifA upstream activator sequence" FT misc_binding 440..451 FT /bound_moiety="IHF" FT /function="potential integration host factor binding site" FT promoter 470..485 FT /function="potential -24/-12 (RpoN-dependent) promoter" FT CDS 655..1533 FT /codon_start=1 FT /note="dinitrogenase reductase (gamma subunit)" FT /transl_table=11 FT /gene="nifH" FT /product="nitrogenase iron protein" FT /protein_id="BAD77945.1" FT /translation="MASLRQIAFYGKGGIGKSTTSQNTLAALAQMGQRILIVGCDPKAD FT STRLILHAKAQDTILSLAADAGSVEDLELEDVMKIGYQNIRCVESGGPEPGVGCAGRGV FT ITSINFLEEEGAYDDTDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMAMYAAN FT NISKGILKYANSGGVRLGGLICNERKTDKELELATALAAKLNSKLIHFVPRDNIVQHAE FT LRRMTVLEYAPDSKQAGEYRTLANKIHENAGNGTIPTPITMDELEDLLMQHGLMPVVDE FT SMVGKSAEVAA" FT CDS 1703..>2855 FT /codon_start=1 FT /note="nitrogenase molybdenum-iron protein (alpha subunit)" FT /transl_table=11 FT /gene="nifD" FT /product="nitrogenase Mo-Fe protein alpha chain" FT /protein_id="BAD77946.1" FT /translation="MSLTVEETTARNTELINEVLKAYPDKTAKRRAKHLTTQEEGKSDC FT NVKSNIKSIPGVMTIPPCAYAGSKGVVWGPIKDMIHISHGPVGCGQYSWGSRRNYYIGK FT TGIDSFVTMQFTSDFQEKDIVFGGDKKLEKIVDEIQELFPLNKGISVQSECPIGLIGDD FT IEAVSKKKSKQYEGHTIVPVRCEGFRGVSQSLGHHVANDAIKEWVLDKMDPDKNQFVAT FT PYDVAIIGDYNIGGDAWSSRILLEEIGLRVIAQWSGDGTLAEMENTPKAKLNVLHCYRS FT MNYISRHMEEKFGIPWVEYNFFGPSQIEASLRQIASHFDDKIKEGAERVIAKYKALTDA FT VIAKYRPRLEGKTVMLFVGGLRPRHVIDAYGDLGMKVVGTGYEF" XX SQ Sequence 2855 BP; 637 A; 841 C; 840 G; 537 T; 0 other; ggtggcggcc gctttagaac tagtggatcc cccgggctgc agcgccaagc cgctgacttc 60 atcacagtcg acgatgaaga cccgtttgtt gtcgaccgcg cgctggctgt ctacaccgat 120 ttgcatggtt tgctccttgc gtgagtggat cactgaccgg gccgcttggc gcagccttct 180 ttgccctgcc attcatcccg atagcaaaca tcgttcccgt tatcagcgag ccgcctctgc 240 ttgtcttcag cagggcgcga caaggcccgt ggctgcgggc cagcgggccg agaggaaagc 300 aacagggtgg ttgggtacac ggttgtgtcg gggatgcggg cttgtatcgt tccctacaga 360 ccaggtgccg tttgtgcggt ttgtaacacc agaaagaagt gggttgagag gctggacgaa 420 atgcagattt caccaattaa atcaatgact tgggatcgtt ttttgatggt ggcacggcat 480 ttgcaaagag aggtgtgcac cgacgatgac ttgccgaatg cgtgacttga gacgaagacc 540 aataaaagtt tcgaagcggc gccccaacgg ctgcaagtgt gatgacaaaa ctgctttgaa 600 cgacatctta atttcttaca ccagagctac accgcttatt tctaggagaa tcaaatggcc 660 tcactcagac aaatcgcctt ctatggcaaa ggcggcatcg gcaagtccac cacctcgcaa 720 aataccctgg ccgcgctggc ccagatgggg cagcgcatcc tcatcgtcgg ctgcgatccc 780 aaggctgact cgacccgcct gatcctgcac gccaaggccc aggacaccat cctgtcgctg 840 gccgccgatg ccgggtcggt ggaggatctg gagctggaag acgtcatgaa gatcggctac 900 cagaacatcc gttgcgtcga atccggcggc ccggaaccgg gcgtgggctg cgctggccgc 960 ggtgtgatca cctcgatcaa cttcctcgaa gaagagggcg cctatgatga caccgattac 1020 gtgtcctacg acgtgctggg cgacgtggta tgcggtggct tcgccatgcc gattcgcgaa 1080 aacaaggcgc aggaaatcta catcgtgatg tccggcgaga tgatggccat gtacgccgcc 1140 aacaacatct ccaagggcat tttgaagtac gccaactccg gtggcgtgcg cctgggcggc 1200 ctgatctgca acgagcgcaa gaccgacaag gaactggaac tggccaccgc cctggcggcc 1260 aagctcaaca gcaagctgat tcacttcgtg ccgcgcgaca acatcgtgca gcacgccgag 1320 ctgcgccgca tgacggtgct ggaatacgca cccgattcca agcaggctgg cgaataccgc 1380 accctggcca acaagatcca cgaaaacgcc ggcaacggca ccatccccac cccgatcacc 1440 atggacgagc tggaagacct gctgatgcag catggcctga tgcctgtcgt tgatgaatcg 1500 atggtgggca agtcggctga agtagcggcc tgagcatcgt cgtctgcctg ctgttgatgc 1560 tgcaaggtcc tggccgctcg cgcctctgtg ttcagaagcg aaggcagccg ggaccgcaag 1620 aaaaagggta gcgcaggttt gtttaccgga ctggcatacg accgggtatg tcccccgccg 1680 actaattttt atcggagggc ccatgagcct cacagttgaa gaaaccacgg cacgcaatac 1740 ggaattgatc aatgaggtcc tcaaggccta tccggacaag accgccaagc gtcgcgccaa 1800 gcacctgacc acccaggaag agggcaagtc ggattgcaac gtcaaatcca acatcaagtc 1860 gatccccggc gtgatgacca ttccgccgtg cgcctatgcc ggttccaagg gcgtggtctg 1920 ggggccgatc aaggacatga tccacatcag ccatggtccg gtcggctgtg gccagtactc 1980 ctggggctcg cgccgcaact attacattgg caagaccggc atcgacagtt tcgtgaccat 2040 gcagttcacg tccgatttcc aggaaaagga catcgtcttc ggcggcgata agaagctgga 2100 aaagatcgtc gacgagatcc aggaactgtt cccgctcaac aagggtattt cggtgcaatc 2160 cgaatgcccg atcggtctca tcggtgacga catcgaagcg gtctccaaga aaaagtccaa 2220 gcaatacgag ggccatacca tcgtgccggt gcgctgcgaa ggcttccgtg gcgtgtcgca 2280 gtcgctgggc caccacgtgg ccaacgacgc catcaaggaa tgggtgctgg acaagatgga 2340 cccggacaag aaccagtttg tggcgactcc gtatgacgtg gccatcatcg gcgactacaa 2400 catcggcggc gacgcctggt cctcgcgcat cctgctggaa gaaatcggcc tgcgcgtgat 2460 cgcccagtgg tccggcgacg gcaccctcgc cgaaatggaa aacaccccca aggccaagct 2520 caacgtgctg cactgctatc gctcgatgaa ctacatctcg cgccacatgg aagagaagtt 2580 cggcattccc tgggtcgagt acaacttctt cggccccagc cagatcgagg cgtcgctgcg 2640 ccagattgcc agccacttcg acgacaagat caaggaaggc gccgagcgtg tcatcgccaa 2700 gtacaaggcc ctcaccgatg ccgtgatcgc caagtaccgc ccgcgcctgg aaggcaagac 2760 cgtgatgctg ttcgtcggtg gcctgcgccc gcgccacgtg atcgatgcct atggtgacct 2820 gggcatgaag gtggtcggta ccggatatga attcg 2855 //