ID O86055_HERSE PRELIMINARY; PRT; 471 AA. AC O86055; DT 01-NOV-1998 (TrEMBLrel. 08, Created) DT 01-NOV-1998 (TrEMBLrel. 08, Last sequence update) DT 01-MAR-2004 (TrEMBLrel. 26, Last annotation update) DE Glutamine synthetase. GN Name=glnA; OS Herbaspirillum seropedicae. OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; OC Oxalobacteraceae; Herbaspirillum. OX NCBI_TaxID=964; RN [1] RP NUCLEOTIDE SEQUENCE. RC STRAIN=Z78; RX MEDLINE=20519424; PubMed=11064198; RA Persuhn D.C., Souza E.M., Steffens M.B.R., Pedrosa F.O., Yates M.G., RA Rigo L.U.; RT "The transcriptional activator NtrC controls the expression and RT activity of glutamine synthetase in Herbaspirillum seropedicae."; RL FEMS Microbiol. Lett. 192:217-221(2000). CC -!- CATALYTIC ACTIVITY: ATP + L-glutamate + NH(3) = ADP + phosphate + CC L-glutamine. CC -!- SUBCELLULAR LOCATION: Cytoplasmic (By similarity). CC -!- SIMILARITY: Belongs to the glutamine synthetase family. DR EMBL; AF082873; AAC32389.1; -. DR HSSP; P06201; 1LGR. DR GO; GO:0005737; C:cytoplasm; IEA. DR GO; GO:0004356; F:glutamate-ammonia ligase activity; IEA. DR GO; GO:0016874; F:ligase activity; IEA. DR GO; GO:0006542; P:glutamine biosynthesis; IEA. DR GO; GO:0009399; P:nitrogen fixation; IEA. DR InterPro; IPR004809; GlnA. DR InterPro; IPR008147; Gln_synt_beta. DR InterPro; IPR008146; Gln_synt_C. DR Pfam; PF00120; Gln-synt_C; 1. DR Pfam; PF03951; Gln-synt_N; 1. DR ProDom; PD001057; Gln_synt_C; 1. DR TIGRFAMs; TIGR00653; GlnA; 1. DR PROSITE; PS00180; GLNA_1; 1. DR PROSITE; PS00181; GLNA_ATP; 1. KW Ligase. SQ SEQUENCE 471 AA; 52209 MW; B85A49AB9DFE6CE2 CRC64; MARTAAEVLK MVKDNEVKFV DFRFADTRGK EQHVSVPVSH FDIDKFESGH AFDGSSIAGW KGIEASDMLL IPDPNTANID PFMEETTLFM QCDVIEPSDG KGYDRDPRSI AKRAEAYLKS SGLGDTAYFG PEPEFFIFDG VRWGADMSGS FVKIDSEEAS WSTGAKIEGG NSGHRPTVKG GYFPVPPVDS FQDMRSEMSL IIESLGIPVE VHHHEVAGAG QNELGTKFST LVERADWTQT MKYVIWNVAH TYGKTATFMP KPLVGDNGSG MHVHQSVWKD GKNLFAGDGY AGLSEFALYY IGGIIKHAKA LNAITNPGTN SYKRLVPGFE APVKLAYSAR NRSASIRIPH VANPKGRRIE TRFPDPLANP YLCFSALLMA GLDGVQNKIH PGEAATKDLY HLPPEEDKLI PTVCSSLDEA LEHLDKDREF LTRGGVFTDS MIDAYIDLKM QEVQRFRMTT HPIEFDMYYS L //