ID   BGAL_KLEPN     STANDARD;      PRT;  1034 AA.
AC   P06219;
DT   01-JAN-1988 (Rel. 06, Created)
DT   01-JAN-1988 (Rel. 06, Last sequence update)
DT   01-FEB-2005 (Rel. 46, Last annotation update)
DE   Beta-galactosidase (EC 3.2.1.23) (Lactase).
GN   Name=lacZ;
OS   Klebsiella pneumoniae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Klebsiella.
OX   NCBI_TaxID=573;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   MEDLINE=85289025; PubMed=3897196;
RA   Buvinger W.E., Riley M.;
RT   "Nucleotide sequence of Klebsiella pneumoniae lac genes.";
RL   J. Bacteriol. 163:850-857(1985).
CC   -!- CATALYTIC ACTIVITY: Hydrolysis of terminal non-reducing beta-D-
CC       galactose residues in beta-D-galactosides.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family.
CC   --------------------------------------------------------------------------
CC   This Swiss-Prot entry is copyright. It is produced through a collaboration
CC   between  the Swiss Institute of Bioinformatics  and the  EMBL outstation -
CC   the European Bioinformatics Institute.  There are no  restrictions on  its
CC   use as long as its content is in no way modified and this statement is not
CC   removed.
CC   --------------------------------------------------------------------------
DR   EMBL; M11441; AAA25082.1; -.
DR   PIR; A24925; A24925.
DR   HSSP; P00722; 1BGL.
DR   InterPro; IPR008979; Gal_bind_like.
DR   InterPro; IPR011013; Gal_mut_like.
DR   InterPro; IPR006101; Glyco_hydro_2.
DR   InterPro; IPR006102; Glyco_hydro_2Ig.
DR   InterPro; IPR006104; Glyco_hydro_2SB.
DR   InterPro; IPR006103; Glyco_hydro_2TIM.
DR   InterPro; IPR004200; Glyco_hydro_42C.
DR   InterPro; IPR004199; Glyco_hydro_42N.
DR   Pfam; PF02930; Bgal_small_C; 1.
DR   Pfam; PF02929; Bgal_small_N; 1.
DR   Pfam; PF00703; Glyco_hydro_2; 1.
DR   Pfam; PF02836; Glyco_hydro_2_C; 1.
DR   Pfam; PF02837; Glyco_hydro_2_N; 1.
DR   PRINTS; PR00132; GLHYDRLASE2.
DR   PROSITE; PS00719; GLYCOSYL_HYDROL_F2_1; 1.
DR   PROSITE; PS00608; GLYCOSYL_HYDROL_F2_2; 1.
KW   Glycosidase; Hydrolase.
FT   ACT_SITE    468    468       Proton donor (By similarity).
FT   ACT_SITE    544    544       Nucleophile (By similarity).
SQ   SEQUENCE   1034 AA;  117517 MW;  8F8B9DC7521EF649 CRC64;
     MQISDTGRSH TPDFHAVLAR EDWHNQTITH LNRLPAHPVF ASWRDELAAR DNLPSSRRRQ
     LDGSGSSLTP AARLPSMRVV TQDLPDCRGT PVPSNWQMEG YDAPIYTNVR YPIDTTPPRV
     PEDNPTGCYS LHFTVEDTWR ENGQTQIIFD GVNSAFHLWC NGVWVGYSQD SRLPAAFDLS
     PFLRPGDNRL CVMVMRWSAG SWLEDQDMWR MSGIFRSVWL LNKPQQRLCD VQLTPALDAL
     YRDGTLQVQA TIEATEAALA GLSVGVSLWR GEEQFAAGRQ PLGTPTVDER GHYAERVDFS
     LAVATPAHWS AETPNCYRAV VTLWRGDELL EAEAWDIGFR RIEIADGLLR LNGKPLLIRG
     VNRHEHHHLR GQVVTEADMV QDILLMKQNN FNAVRCSHYP NAPRWYELCN RYGLYVVDEA
     NIETHGMVPM NRLSDDPAWL PAFSARVTRM VQSNRNHPCI IIWSLGNESG GGGNHEALYH
     WLKRNDPSRP VQYEGGGADT TATDIICPMY ARVERDQPIP AVPKWGIKKW ISLPGEQRPL
     ILCEYAHAMG NSLGNFADYW QAFREYPRLQ GGFIWDWADQ AIRKTFADGS VGWAYGGDFG
     DKPNDRQFCM NGLVFPDRTP HPSLVEAKHA QQYFQFTLLS TSPLRVRIIS EYLFRPTDNE
     VVRWQVQAAG EPLYHGDLTL ALPPEGSDEI TLLDSLILPE GARAVWLTLE VTQPQATAWS
     EAEHRVAWQQ FPLPAPLGCR RPPCLPALPD LIVSDEVWQI RAGSQCWTID RRTGLLSRWS
     VGGQEQLLTP LRDQFIRAPL DNDIGVSEVE RIDPNAWVER WRSAGLYDLE AHCVQCDAQR
     LANETLVDCR WHYLRGEEVV IVSHWRMHFT ADGTLRLAVD GERAETLPPL PRVGLHFQVA
     DQQAPVSWLG LGPHENYPDR RSSACFARWE QPLAAMTTPY IFPTENGLRC DTQALDWGRW
     HISGHFHFSV QPWSTRQLME TDHWHKMQAE DGVWITLDGL HMGVGGDDSW TPSVLPQWLL
     SQTRWQYEVS LRSL
//