ID   CDGT_KLEOX     STANDARD;      PRT;   655 AA.
AC   P08704;
DT   01-JAN-1988 (Rel. 06, Created)
DT   01-JAN-1988 (Rel. 06, Last sequence update)
DT   01-FEB-2005 (Rel. 46, Last annotation update)
DE   Cyclomaltodextrin glucanotransferase precursor (EC 2.4.1.19)
DE   (Cyclodextrin-glycosyltransferase) (CGTase).
GN   Name=cgt;
OS   Klebsiella oxytoca.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Klebsiella.
OX   NCBI_TaxID=571;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=M5a1;
RX   MEDLINE=87163498; PubMed=2951300; DOI=10.1016/0378-1119(86)90070-3;
RA   Binder F., Huber O., Boeck A.;
RT   "Cyclodextrin-glycosyltransferase from Klebsiella pneumoniae M5a1:
RT   cloning, nucleotide sequence and expression.";
RL   Gene 47:269-277(1986).
CC   -!- CATALYTIC ACTIVITY: Cyclizes part of a 1,4-alpha-D-glucan chain by
CC       formation of a 1,4-alpha-D-glucosidic bond.
CC   -!- COFACTOR: Binds 2 calcium ions per subunit (By similarity).
CC   -!- SUBUNIT: Monomer.
CC   -!- DOMAIN: May consist of two protein domains: the one in the N-
CC       terminal side cleaves the alpha-1,4-glucosidic bond in starch, and
CC       the other in the C-terminal side catalyzes other activities,
CC       including the reconstitution of an alpha-1,4-glucosidic linkage
CC       for cyclizing the maltooligosaccharide produced.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC   --------------------------------------------------------------------------
CC   This Swiss-Prot entry is copyright. It is produced through a collaboration
CC   between  the Swiss Institute of Bioinformatics  and the  EMBL outstation -
CC   the European Bioinformatics Institute.  There are no  restrictions on  its
CC   use as long as its content is in no way modified and this statement is not
CC   removed.
CC   --------------------------------------------------------------------------
DR   EMBL; M15264; AAA25059.1; -.
DR   PIR; A29023; ALKBG.
DR   HSSP; P19531; 1QHP.
DR   InterPro; IPR006589; Alp_amyl_cat_sub.
DR   InterPro; IPR006048; Alpha_amyl_C.
DR   InterPro; IPR006047; Alpha_amyl_cat.
DR   InterPro; IPR002044; Glyco_hydro_CBD.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   Pfam; PF02806; Alpha-amylase_C; 1.
DR   Pfam; PF00686; CBM_20; 1.
DR   ProDom; PD001568; Glyco_hydro_CBD; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SMART; SM00632; Aamy_C; 1.
KW   Calcium-binding; Glycosyltransferase; Signal; Transferase.
FT   SIGNAL        1     30
FT   CHAIN        31    655       Cyclomaltodextrin glucanotransferase.
FT   ACT_SITE    253    253       Nucleophile (By similarity).
FT   ACT_SITE    287    287       Proton donor (By similarity).
FT   ACT_SITE    363    363       By similarity.
FT   METAL        55     55       Calcium 2 (By similarity).
FT   METAL        60     60       Calcium 2 (By similarity).
FT   METAL        61     61       Calcium 2 (By similarity).
FT   METAL        79     79       Calcium 2 (via carbonyl oxygen) (By
FT                                similarity).
FT   METAL        81     81       Calcium 2 (By similarity).
FT   METAL       164    164       Calcium 1 (By similarity).
FT   METAL       223    223       Calcium 1 (By similarity).
FT   METAL       257    257       Calcium 1 (via carbonyl oxygen) (By
FT                                similarity).
SQ   SEQUENCE   655 AA;  73025 MW;  DB8F26332BED26A7 CRC64;
     MKRNRFFNTS AAIAISIALN TFFCSMQTIA AEPEETYLDF RKETIYFLFL DRFSDGDPSN
     NAGFNSATYD PNNLKKYTGG DLRGLINKLP YLKSLGVTSI WITPPIDNVN NTDAAGNTGY
     HGYWGRDYFR IDEHFGNLDD FKELTSLMHS PDYNMKLVLD YAPNHSNAND ENEFGALYRD
     GVFITDYPTN VAANTGWYHH NGGVTNWNDF FQVKNHNLFN LSDLNQSNTD VYQYLLDGSK
     FWIDAGVDAI RIDAIKHMDK SFIQKWTSDI YDYSKSIGRE GFFFFGEWFG ASANTTTGVD
     GNAIDYANTS GSALLDFGFR DTLERVLVGR SGNTMKTLNS YLIKRQTVFT SDDWQVVFMD
     NHDMARIGTA LRSNATTFGP GNNETGGSQS EAFAQKRIDL GLVATMTVRG IPAIYYGTEH
     YAANFTSNSF GQVGSDPYNR EKMPGFDTES EAFSIIKTLG DLRKSSPAIQ NGTYTELWVN
     DDILVFERRS GNDIVIVALN RGEANTINVK NIAVPNGVYP SLIGNNSVSV ANKRTTLTLM
     QNEAVVIRSQ SDDAENPTVQ SINFTCNNGY TISGQSVYII GNIPQLGGWD LTKAVKISPT
     QYPQWSASLE LPSDLNVEWK CVKRNETNPT ANVEWQSGAN NQFNSNDTQT TNGSF
//