ID   AADB_ECOLI     STANDARD;      PRT;   177 AA.
AC   P10019; P08880;
DT   01-NOV-1988 (Rel. 09, Created)
DT   01-NOV-1988 (Rel. 09, Last sequence update)
DT   05-JUL-2004 (Rel. 44, Last annotation update)
DE   2''-aminoglycoside nucleotidyltransferase (EC 2.7.7.46) (AAD(2''))
DE   (Gentamicin 2''-nucleotidyltransferase) (Gentamicin resistance
DE   protein).
GN   Name=aadB;
OS   Escherichia coli, and
OS   Klebsiella pneumoniae.
OG   Plasmid pDGO100, Plasmid IncQ pIE723, Plasmid pBP201, and
OG   Plasmid IncM pBWH1.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562, 573;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   SPECIES=E.coli; PLASMID=pDGO100;
RX   MEDLINE=87066734; PubMed=3024112;
RA   Cameron F.H., Groot Obbink D.J., Ackerman V.P., Hall R.M.;
RT   "Nucleotide sequence of the AAD(2'') aminoglycoside
RT   adenylyltransferase determinant aadB. Evolutionary relationship of
RT   this region with those surrounding aadA in R538-1 and dhfrII in
RT   R388.";
RL   Nucleic Acids Res. 14:8625-8635(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RC   SPECIES=E.coli; STRAIN=O147:K88 / IE1003; PLASMID=IncQ pIE723;
RA   Tietze E.;
RL   Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE.
RC   SPECIES=K.pneumoniae; STRAIN=18440; PLASMID=pBP201; TRANSPOSON=Tn4000;
RX   MEDLINE=89096491; PubMed=2850441;
RA   Schmidt F.R.J., Nuecken E.J., Henschke R.B.;
RT   "Nucleotide sequence analysis of 2''-aminoglycoside nucleotidyl-
RT   transferase ANT(2'') from Tn4000: its relationship with AAD(3'') and
RT   impact on Tn21 evolution.";
RL   Mol. Microbiol. 2:709-717(1988).
RN   [4]
RP   NUCLEOTIDE SEQUENCE.
RC   SPECIES=K.pneumoniae; PLASMID=IncM pBWH1;
RA   Zieg J., Jiang H., McCabe F., O'Brien T.;
RL   Submitted (FEB-1992) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: This enzyme confers resistance to kanamycin, gentamicin,
CC       dibekacin, sisomicin, neomycin and tobramycin by adenylating the
CC       2''-hydroxyl group of these antibiotics.
CC   -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + gentamicin =
CC       diphosphate + 2''-nucleotidylgentamicin.
CC   --------------------------------------------------------------------------
CC   This Swiss-Prot entry is copyright. It is produced through a collaboration
CC   between  the Swiss Institute of Bioinformatics  and the  EMBL outstation -
CC   the European Bioinformatics Institute.  There are no  restrictions on  its
CC   use as long as its content is in no way modified and this statement is not
CC   removed.
CC   --------------------------------------------------------------------------
DR   EMBL; L06418; AAA92745.1; -.
DR   EMBL; M86913; AAA72105.1; -.
DR   EMBL; Z47410; CAA87463.1; -.
DR   EMBL; U14415; AAA85811.1; -.
DR   EMBL; X04555; CAA28209.1; ALT_INIT.
DR   EMBL; X12618; CAA31139.1; -.
DR   EMBL; X64368; CAA45721.1; -.
DR   PIR; A25536; XNECAD.
DR   PIR; S04170; XNKBGP.
KW   Antibiotic resistance; Nucleotidyltransferase; Plasmid; Transferase;
KW   Transposable element.
SQ   SEQUENCE   177 AA;  19873 MW;  9A084D96AE99F2F1 CRC64;
     MDTTQVTLIH KILAAADERN LPLWIGGGWA IDARLGRVTR KHDDIDLTFP GERRGELEAI
     VEMLGGRVME ELDYGFLAEI GDELLDCEPA WWADEAYEIA EAPQGSCPEA AEGVIAGRPV
     RCNSWEAIIW DYFYYADEVP PVDWPTKHIE SYRLACTSLG AEKVEVLRAA FRSRYAA
//