ID   AROA_KLEPN     STANDARD;      PRT;   427 AA.
AC   P24497;
DT   01-MAR-1992 (Rel. 21, Created)
DT   01-MAR-1992 (Rel. 21, Last sequence update)
DT   05-JUL-2004 (Rel. 44, Last annotation update)
DE   3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) (5-
DE   enolpyruvylshikimate-3-phosphate synthase) (EPSP synthase) (EPSPS).
GN   Name=aroA;
OS   Klebsiella pneumoniae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Klebsiella.
OX   NCBI_TaxID=573;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   MEDLINE=91053165; PubMed=2241161;
RA   Sost D., Amrhein N.;
RT   "Substitution of Gly-96 to Ala in the 5-enolpyruvylshikimate-3-
RT   phosphate synthase of Klebsiella pneumoniae results in a greatly
RT   reduced affinity for the herbicide glyphosate.";
RL   Arch. Biochem. Biophys. 282:433-436(1990).
CC   -!- CATALYTIC ACTIVITY: Phosphoenolpyruvate + 3-phosphoshikimate =
CC       phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate.
CC   -!- PATHWAY: Aromatic amino acids biosynthesis; shikimate pathway;
CC       sixth step.
CC   -!- SUBUNIT: Monomer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic (Probable).
CC   -!- SIMILARITY: Belongs to the EPSP synthase family.
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CC   This Swiss-Prot entry is copyright. It is produced through a collaboration
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DR   EMBL; X82415; CAA57812.1; -.
DR   PIR; S13266; S13266.
DR   HSSP; P07638; 1G6S.
DR   HAMAP; MF_00210; -; 1.
DR   InterPro; IPR006264; AroA.
DR   InterPro; IPR001986; EPSP_synth.
DR   Pfam; PF00275; EPSP_synthase; 1.
DR   ProDom; PD001867; EPSP_synth; 1.
DR   TIGRFAMs; TIGR01356; aroA; 1.
DR   PROSITE; PS00104; EPSP_SYNTHASE_1; 1.
DR   PROSITE; PS00885; EPSP_SYNTHASE_2; 1.
KW   Aromatic amino acid biosynthesis; Herbicide resistance; Transferase.
FT   MUTAGEN      96     96       G->A: Reduced affinity for herbicide
FT                                glyphosate.
SQ   SEQUENCE   427 AA;  46029 MW;  01AF6E750FC710FF CRC64;
     MESLTLQPIA RVDGTVNLPG SKSVSNRALL LAALARGTTV LTNLLDSDDV RHMLNALSAL
     GVHYVLSSDR TRCEVTGTGG PLQAGSALEL FLGNAGTAMR PLAAALCLGS NDIVLTGEPR
     MKERPIGHLV DALRQGGAQI DYLEQENYPP LRLRGGFTGG DVEVDGSVSS QFLTALLMAS
     PLAPQDTVIA IKGELVSRPY IDITLHLMKT FGVEVENQAY QRFIVRGNQQ YQSPGDYLVE
     GDASSASYFL AAGAIKGGTV KVTGIGRNSV QGDIRFADVL EKMGATVTWG EDYIACTRGE
     LNAIDMDMNH IPDAAMTIAT AALFARGTTT LRNIYNWRVK ETDRLFAMAT ELRKVGAEVE
     EGEDYIRITP PLTLQFAEIG TYNDHRMAMC FSLVALSDTP VTILDPKCTA KTFPDYFGQL
     ARISTLA
//