ID   ALYA_KLEPN     STANDARD;      PRT;   307 AA.
AC   Q59478;
DT   15-DEC-1998 (Rel. 37, Created)
DT   15-DEC-1998 (Rel. 37, Last sequence update)
DT   01-FEB-2005 (Rel. 46, Last annotation update)
DE   Alginate lyase precursor (EC 4.2.2.3) (Poly(beta-D-mannuronate) lyase)
DE   (Poly(mana) alginate lyase).
GN   Name=alyA;
OS   Klebsiella pneumoniae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Klebsiella.
OX   NCBI_TaxID=573;
RN   [1]
RP   NUCLEOTIDE SEQUENCE, AND PROTEIN SEQUENCE OF 21-51.
RC   STRAIN=Subsp. aerogenes 25;
RX   MEDLINE=94259303; PubMed=8200539; DOI=10.1016/0378-1119(94)90605-X;
RA   Baron A.J., Wong T.Y., Hicks S.J., Gacesa P., Willcock D.,
RA   McPherson M.J.;
RT   "Alginate lyase from Klebsiella pneumoniae, subsp. aerogenes: gene
RT   cloning, sequence analysis and high-level production in Escherichia
RT   coli.";
RL   Gene 143:61-66(1994).
CC   -!- FUNCTION: Degrades alginates that contain guluronic acid.
CC   -!- CATALYTIC ACTIVITY: Eliminative cleavage of polysaccharides
CC       containing beta-D-mannuronate residues to give oligosaccharides
CC       with 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl groups at
CC       their ends.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the polysaccharide lyase 7 family.
CC   --------------------------------------------------------------------------
CC   This Swiss-Prot entry is copyright. It is produced through a collaboration
CC   between  the Swiss Institute of Bioinformatics  and the  EMBL outstation -
CC   the European Bioinformatics Institute.  There are no  restrictions on  its
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DR   EMBL; L19657; AAA25049.1; -.
DR   InterPro; IPR008397; Alginate_lyase.
DR   Pfam; PF05426; Alginate_lyase; 1.
KW   Direct protein sequencing; Lyase; Signal.
FT   SIGNAL        1     20
FT   CHAIN        21    307       Alginate lyase.
SQ   SEQUENCE   307 AA;  33512 MW;  76A1F9AAE082632E CRC64;
     MLKSGVMVAS LCLFSVPSRA AVPAPGDKFE LSGWSLSVPV DSDNDGKADQ IKEKTLAAGY
     RNSDFFTLSD AGGMVFKAPI SGAKTSKNTT YTRSELREML RKGDTSIATQ GVSRNNWVLS
     SAPLSEQKKA GGVDGTLEAT LSVDHVTTTG VNWQVGRVII GQIHANNDEP IRLYYRKLPH
     HQKGSVYFAH EPRKGFGDEQ WYEMIGTLQP SHGNQTAAPT EPEAGIALGE TFSYRIDATG
     NKLTVTLMRE GRPDVVKTVD MSKSGYSEAG QYLYFKAGVY NQNKTGKPDD YVQATFYRLK
     ATHGAQR
//