ID   AGLB_KLEPN     STANDARD;      PRT;   440 AA.
AC   Q9AGA6;
DT   28-FEB-2003 (Rel. 41, Created)
DT   28-FEB-2003 (Rel. 41, Last sequence update)
DT   01-FEB-2005 (Rel. 46, Last annotation update)
DE   6-phospho-alpha-glucosidase (EC 3.2.1.122).
GN   Name=aglB;
OS   Klebsiella pneumoniae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Klebsiella.
OX   NCBI_TaxID=573;
RN   [1]
RP   NUCLEOTIDE SEQUENCE, PROTEIN SEQUENCE OF 1-26, AND MASS SPECTROMETRY.
RC   STRAIN=ATCC 23357;
RX   MEDLINE=21463057; PubMed=11473129; DOI=10.1074/jbc.M106504200;
RA   Thompson J., Robrish S.A., Immel S., Lichtenthaler F.W., Hall B.G.,
RA   Pikis A.;
RT   "Metabolism of sucrose and its five linkage-isomeric alpha-D-glucosyl-
RT   D-fructoses by Klebsiella pneumoniae. Participation and properties of
RT   sucrose-6-phosphate hydrolase and phospho-alpha-glucosidase.";
RL   J. Biol. Chem. 276:37415-37425(2001).
CC   -!- FUNCTION: Hydrolyzes a wide variety of 6-phospho-alpha-D-
CC       glucosides including maltose-6'P, isomaltose-6'P, maltitol-6P,
CC       trehalose-6P and the 6'-phosphorylated derivatives of the five
CC       linkage-isomeric alpha-D-glucosyl-D-fructoses: trehalulose-6'P,
CC       turanose-6'P, maltulose-6'P, leucrose-6'P, and palatinose-6'P.
CC       However, sucrose-6P is not a substrate for this enzyme.
CC   -!- CATALYTIC ACTIVITY: Maltose 6'-phosphate + H(2)O = D-glucose + D-
CC       glucose 6-phosphate.
CC   -!- COFACTOR: NAD and a divalent metal ion. Manganese, cobalt and
CC       nickel ions enhance activity whereas magnesium, calcium, and zinc
CC       ions are without effect or inhibitory.
CC   -!- PATHWAY: Sucrose isomers metabolism.
CC   -!- SUBUNIT: Homodimer.
CC   -!- INDUCTION: By the five linkage-isomeric alpha-D-glucosyl-D-
CC       fructoses, or by maltose or maltitol.
CC   -!- MASS SPECTROMETRY: MW=49254; METHOD=Electrospray; RANGE=1-440;
CC       NOTE=Ref.1.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 4 family.
CC   --------------------------------------------------------------------------
CC   This Swiss-Prot entry is copyright. It is produced through a collaboration
CC   between  the Swiss Institute of Bioinformatics  and the  EMBL outstation -
CC   the European Bioinformatics Institute.  There are no  restrictions on  its
CC   use as long as its content is in no way modified and this statement is not
CC   removed.
CC   --------------------------------------------------------------------------
DR   EMBL; AF337811; AAK01457.1; -.
DR   InterPro; IPR001088; Glyco_hydro_4.
DR   Pfam; PF02056; Glyco_hydro_4; 1.
DR   PRINTS; PR00732; GLHYDRLASE4.
DR   ProDom; PD006892; Glyco_hydro_4; 1.
DR   PROSITE; PS01324; GLYCOSYL_HYDROL_F4; 1.
KW   Carbohydrate metabolism; Cobalt; Direct protein sequencing;
KW   Glycosidase; Hydrolase; Manganese; NAD; Nickel.
FT   NP_BIND       5     39       NAD (Potential).
FT   ACT_SITE     39     39       By similarity.
FT   ACT_SITE    109    109       Proton donor (By similarity).
FT   ACT_SITE    357    357       Nucleophile (By similarity).
SQ   SEQUENCE   440 AA;  49255 MW;  5D7287435E4DDC45 CRC64;
     MKKFSVVIAG GGSTFTPGIV LMLLANQDRF PLRSLKFYDN DGARQETIAE ACKVILKEQA
     PEIEFSYTTD PQAAFTDVDF VMAHIRVGKY PMREQDEKIP LRHGVLGQET CGPGGIAYGM
     RSIGGVLELV DYMEKYSPNA WMLNYSNPAA IVAEATRRLR PNAKILNICD MPIGIEGRMA
     QIVGLKDRKQ MRVRYYGLNH FGWWTSIEDL DGNDLMPKLR EYVAKYGYVP PSNDPHTEAS
     WNDTFAKAKD VQALDPQTMP NTYLKYYLFP DYVVAHSNPE RTRANEVMDH REKNVFSACR
     AIIAAGKSTA GDLEIDEHAS YIVDLATAIA FNTQERMLLI VPNNGAIHNF DADAMVEIPC
     LVGHNGPEPL TVGDIPHFQK GLMSQQVAVE KLVVDAWEQR SYHKLWQAIT LSKTVPSASV
     AKAILDDLIA ANKDYWPELH
//