ID   CV222141   standard; mRNA; EST; 672 BP.
XX
AC   CV222141;
XX
SV   CV222141.1
XX
DT   17-SEP-2004 (Rel. 81, Created)
DT   17-SEP-2004 (Rel. 81, Last updated, Version 1)
XX
DE   Le_mx0_56h05_SP6 Little Skate Multiple Tissues, Normalized Leucoraja
DE   erinacea cDNA clone Le_mx0_56h05 5' similar to gb|AAL13172.2| recQ family
DE   helicase - Metarhizium anisopliae. Score = 32.7 bits (73), Expect = 6.8,
DE   mRNA sequence.
XX
KW   EST.
XX
OS   Leucoraja erinacea (little skate)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Chondrichthyes;
OC   Elasmobranchii; Squalea; Hypnosqualea; Pristiorajea; Batoidea; Rajiformes;
OC   Rajidae; Leucoraja.
XX
RN   [1]
RP   1-672
RA   Towle D.W., Smith C.M.;
RT   "Expressed sequence tags in a normalized cDNA library prepared from
RT   multiple tissues of adult little skate, Leucoraja erinacea";
RL   Unpublished.
XX
DR   UNILIB; 35911; 15890.
XX
CC   Contact: David W. Towle
CC   Marine DNA Sequencing and Analysis Center
CC   Mount Desert Island Biological Laboratory
CC   Old Bar Harbor Road, Salsbury Cove, ME 04672 USA
CC   Tel: 207-288-9880 x474
CC   Fax: 207-288-2130
CC   Email: dtowle@mdibl.org
CC   Plate: 56  row: h  column: 05
CC   Seq primer: SP6
CC   High quality sequence stop: 507.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..672
FT                   /db_xref="taxon:7782"
FT                   /db_xref="UNILIB:35911"
FT                   /mol_type="mRNA"
FT                   /note="Vector: pCMV Sport 6.1; Total RNA samples were
FT                   prepared individually from each tissue, checked for
FT                   quality, then pooled for construction and normalization of
FT                   cDNA library by Invitrogen. Plasmids were isolated and
FT                   inserts end-sequenced by the Marine DNA Sequencing and
FT                   Analysis Facility at Mount Desert Island Biological
FT                   Laboratory. Traces were processed for submission to dbEST
FT                   by trace2dbest software (Parkinson, Anthony and Blaxter,
FT                   unpublished software)."
FT                   /organism="Leucoraja erinacea"
FT                   /clone="Le_mx0_56h05"
FT                   /tissue_type="Liver, kidney, brain, testis, ovary, gill,
FT                   heart, spleen, rectal gland"
FT                   /dev_stage="Adult"
FT                   /clone_lib="Little Skate Multiple Tissues, Normalized"
XX
SQ   Sequence 672 BP; 201 A; 163 C; 133 G; 175 T; 0 other;
     caccattctt tttggccaaa tcggtggtgg tgatggatca gtttgcagga gagaaaacat        60
     ctaattgatt gagtggtgcc acaacaacaa cctctcgctc aatgtcaaca aaaccaatga       120
     cttcagaaaa gggacgttgg aagaccatgt accagttttc attggtgagc ccccaatgga       180
     gtaagatagt agctcccaat tccagagttt taacatctca aatgaagaag ggtctcgact       240
     cgaaacgtca cctattcttt ctatccaaag atgctgcctg tcccactgag ctactccagc       300
     tttttgtggc taacatctga aatgatctgt cctgggccca gtacatagat tgtaatcatg       360
     aagacgtgcc atgcatttac attagtagaa gtttaaaaag atttagcatc tcaccgaata       420
     ctccaacaaa ccttgacagt aaaatgcact ataaaaagca tcctgactgt ttgcatcatg       480
     atcttgtttg gcaataaaaa acacacaggc tgtgtggcaa ttaaaaacac actgcagcgt       540
     gactctgcag tggtggtctc agccccgtcc ttcacaggca ctgacatcca tccagagcat       600
     ctacatgagg ggctgcctga agaaggatct acaacatcca agccttgcct ttttctcact       660
     gctactactc ac                                                           672
//