ID   CUDP_METAN     STANDARD;      PRT;   388 AA.
AC   P29138;
DT   01-DEC-1992 (Rel. 24, Created)
DT   01-DEC-1992 (Rel. 24, Last sequence update)
DT   25-OCT-2004 (Rel. 45, Last annotation update)
DE   Cuticle-degrading protease precursor (EC 3.4.21.-) (PR1)
DE   (Chymoelastase).
GN   Name=PR1;
OS   Metarhizium anisopliae.
OC   Eukaryota; Fungi; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Clavicipitaceae;
OC   mitosporic Clavicipitaceae; Metarhizium.
OX   NCBI_TaxID=5530;
RN   [1]
RP   NUCLEOTIDE SEQUENCE, AND PROTEIN SEQUENCE OF 108-115.
RC   STRAIN=ME1 / ARSEF 2575;
RX   MEDLINE=92201213; PubMed=1551399;
RA   St Leger R.J., Frank D.C., Roberts D.W., Staples R.C.;
RT   "Molecular cloning and regulatory analysis of the cuticle-degrading-
RT   protease structural gene from the entomopathogenic fungus Metarhizium
RT   anisopliae.";
RL   Eur. J. Biochem. 204:991-1001(1992).
CC   -!- FUNCTION: Capable of breaching the insect cuticle.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the peptidase S8 family.
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CC   This Swiss-Prot entry is copyright. It is produced through a collaboration
CC   between  the Swiss Institute of Bioinformatics  and the  EMBL outstation -
CC   the European Bioinformatics Institute.  There are no  restrictions on  its
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DR   EMBL; M73795; AAA33417.1; -.
DR   PIR; S22387; S22387.
DR   HSSP; P06873; 1IC6.
DR   MEROPS; S08.056; -.
DR   InterPro; IPR000209; Pept_S8_S53.
DR   InterPro; IPR009020; Prot_inh_propept.
DR   InterPro; IPR010259; Prot_inh_S8A.
DR   Pfam; PF00082; Peptidase_S8; 1.
DR   Pfam; PF05922; Subtilisin_N; 1.
DR   PRINTS; PR00723; SUBTILISIN.
DR   PROSITE; PS00136; SUBTILASE_ASP; 1.
DR   PROSITE; PS00137; SUBTILASE_HIS; 1.
DR   PROSITE; PS00138; SUBTILASE_SER; 1.
KW   Direct protein sequencing; Glycoprotein; Hydrolase; Serine protease;
KW   Signal; Zymogen.
FT   SIGNAL        1     18       Potential.
FT   PROPEP       19    107       Potential.
FT   CHAIN       108    388       Cuticle-degrading protease.
FT   ACT_SITE    148    148       Charge relay system (By similarity).
FT   ACT_SITE    179    179       Charge relay system (By similarity).
FT   ACT_SITE    334    334       Charge relay system (By similarity).
FT   DISULFID    143    233       By similarity.
FT   DISULFID    288    360       By similarity.
FT   CARBOHYD    296    296       N-linked (GlcNAc...) (Potential).
SQ   SEQUENCE   388 AA;  40331 MW;  4CAF735D3FA39352 CRC64;
     MHLSALLTLL PAVLAAPATI GRRAEPAPLF TPQAESIIAD KYIVKFKDDI ARIATDDTVS
     ALTSKADFVY EHAFHGFAGS LTKEELKMLR EHPGVDFIEK DAVMRISGIT EQSGAPWGLG
     RISHRSKGST TYRYDDSAGQ GTCVYIIDTG IEASHPEFEG RATFLKSFIS GQNTDGHGHG
     THCAGTIGSK TYGVAKKAKL YGVKVLDNQG SGSYSGIISG MDYVAQDSKT RGCPNGAIAS
     MSLGGGYSAS VNQGAAALVN SGVFLAVAAG NDNRDAQNTS PASEPSACTV GASAENDSRS
     SFSNYGRVVD IFAPGSNVLS TWIVGRTNSI SGTSMATPHI AGLAAYLSAL QGKTTPAALC
     KKIQDTATKN VLTGVPSGTV NYLAYNGA
//