ID   ACST3HECT  standard; genomic DNA; INV; 339 BP.
XX
AC   X58376; S39755;
XX
SV   X58376.1
XX
DT   09-JUN-1992 (Rel. 32, Created)
DT   21-MAR-2000 (Rel. 63, Last updated, Version 8)
XX
DE   Recombinant baculovirus AcST-3 DNA for Scorpian Hector insect toxin (AaHIT)
DE   with A.californica nuclear polyhedrosis virus (AcNPV) gp67 signal peptide
DE   sequence
XX
KW   AaHIT protein; expression vector; insect-specific; neurotoxin; polyhedrin;
KW   recombinant protein.
XX
OS   synthetic construct
OC   other sequences; artificial sequences.
OG   Plasmid pAcUW2B
XX
OS   Androctonus australis (Sahara scorpion)
OC   Eukaryota; Metazoa; Arthropoda; Chelicerata; Arachnida; Scorpiones;
OC   Buthida; Buthoidea; Buthidae; Androctonus.
XX
RN   [1]
RP   1-339
RX   DOI; 10.1038/352085a0.
RX   MEDLINE; 91287829.
RX   PUBMED; 2062383.
RA   Stewart L.M., Hirst M., Lopez Ferber M, Merryweather A.T., Cayley P.J.,
RA   Possee R.D.;
RT   "Construction of an improved baculovirus insecticide containing an
RT   insect-specific toxin gene.";
RL   Nature 352(6330):85-88(1991).
XX
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..339
FT                   /db_xref="taxon:32630"
FT                   /mol_type="genomic DNA"
FT                   /organism="synthetic construct"
FT                   /plasmid="pAcUW2B"
FT   source          7..333
FT                   /db_xref="taxon:6858"
FT                   /focus
FT                   /mol_type="genomic DNA"
FT                   /organism="Androctonus australis"
FT   sig_peptide     7..120
FT                   /note="AcNPV gp67 signal peptide"
FT                   /note="Autographa californica nuclear polyhedrosis virus
FT                   (ACNPV)"
FT   CDS             7..333
FT                   /gene="AaHIT"
FT                   /product="Hector insect toxin"
FT                   /protein_id="CAA41265.1"
FT                   /translation="MLLVNQSHQGFNKEHTSKMVSAIVLYVLLAAAAHSAFAKKNGYAV
FT                   DSSGKAPECLLSNYCNNQCTKVHYADKGYCCLLSCYCFGLNDDKKVLEISDTRKSYCDT
FT                   TIIN"
FT   mat_peptide     121..330
FT                   /gene="AaHIT"
FT                   /product="Hector insect toxin"
XX
SQ   Sequence 339 BP; 108 A; 74 C; 69 G; 88 T; 0 other;
     agatctatgc tactagtaaa tcagtcacac caaggcttca ataaggaaca cacaagcaag        60
     atggtaagcg ctattgtttt atatgtgctt ttggcggcgg cggcgcattc tgcctttgcg       120
     aaaaaaaacg gctacgcggt tgactcgtcg ggcaaagcgc cagaatgtct actatcgaac       180
     tactgtaaca accaatgtac taaagttcac tacgctgaca aaggctactg ttgtctacta       240
     agctgttact gttttggcct aaacgacgac aaaaaagttc tagaaattag cgacactcgt       300
     aaaagctact gtgacactac tattattaac taaagatct                              339
//