ID AY686700 standard; genomic DNA; FUN; 3460 BP. XX AC AY686700; XX SV AY686700.1 XX DT 13-DEC-2004 (Rel. 82, Created) DT 13-DEC-2004 (Rel. 82, Last updated, Version 1) XX DE Pleurotus eryngii laccase precursor (pel3) gene, complete cds. XX KW . XX OS Pleurotus eryngii OC Eukaryota; Fungi; Basidiomycota; Hymenomycetes; Homobasidiomycetes; OC Agaricales; Pleurotaceae; Pleurotus. XX RN [1] RP 1-3460 RA Rodriguez E., Martinez A.T., Martinez M.J.; RT "Cloning and characterization of a new laccase from the white rot fungus RT Pleurotus eryngii"; RL Unpublished. XX RN [2] RP 1-3460 RA Rodriguez E., Martinez A.T., Martinez M.J.; RT ; RL Submitted (15-JUL-2004) to the EMBL/GenBank/DDBJ databases. RL Molecular Microbiology, Centro de Investigaciones Biologicas (CSIC), Ramiro RL de Maeztu, 9, Madrid 28040, Spain XX FH Key Location/Qualifiers FH FT source 1..3460 FT /db_xref="taxon:5323" FT /mol_type="genomic DNA" FT /organism="Pleurotus eryngii" FT gene <763..>3405 FT /gene="pel3" FT mRNA join(<763..899,951..1035,1087..1155,1204..1246,1293..1341, FT 1391..1428,1479..1536,1592..1638,1693..1721,1778..2018, FT 2082..2105,2156..2239,2293..2511,2569..2592,2646..2776, FT 2834..2854,2908..3059,3113..3136,3186..3238,3301..>3368) FT /gene="pel3" FT /product="laccase precursor" FT CDS join(763..899,951..1035,1087..1155,1204..1246,1293..1341, FT 1391..1428,1479..1536,1592..1638,1693..1721,1778..2018, FT 2082..2105,2156..2239,2293..2511,2569..2592,2646..2776, FT 2834..2854,2908..3059,3113..3136,3186..3238,3301..3368) FT /codon_start=1 FT /note="oxidoreductase; blue copper oxidase" FT /gene="pel3" FT /EC_number="1.10.3.2" FT /product="laccase precursor" FT /protein_id="AAV85769.1" FT /translation="MFPGARILATLTLALHLLHGTHAAIGPIADMYIVNEDVSPDGFAR FT SAVVARSVPATDPTPASVSVPGVLVQGNKGDNFQLNVRNQLSDSTMLKTTSIHWHGFFQ FT SGSTWADGPAFVNQCPIASGNSFLYDFNVPDQAGTFWYHSHLSTQYCDGLRGPFVVYDP FT SDPHLSLYDVDNADTVITLEDWYHVAAPQGAVLPTPDSTLINGKGRFAGGPTSPLAIIN FT VESNKRYRFRLISMSCDPNFTFSIDGHSLQVIEADAVNIVPIVVDSIQIFAGQRYSFVL FT NANQAVDNYWIRANPNLGSTGFEGGINSAILRYAGATENDPATTSSTSTPLLETNLVPL FT ENPGAPGPAVPGGADININLAMGFDFTNFEMTINGSPFKAPTAPVLLQILSGATPAASL FT LPSGSIYALAANKVVEISIPALAVGGPHPFHLHGHTFDVIRSAGSTTYNFDTPARRDVV FT NTGIDANDNVTIRFVTDNPGPWFLHCHIDWHLEIGLAVVFAEDVASIKAPPAAWDDLCP FT IYDALSDSDRGGIA" FT sig_peptide 763..831 FT /gene="pel3" FT polyA_signal 3400..3405 FT /gene="pel3" XX SQ Sequence 3460 BP; 774 A; 967 C; 763 G; 956 T; 0 other; ctgtgaggaa cggggattca tcgacgaccg tgttgactac tcgactggcc gtaagcacag 60 gagcgtcttg cgcaccctgc atcaacgttg gcgttagcgt agcgacgaag catggccttc 120 ccgacttacg acgaaggcag agatggcgca gatggtgatg atgagcgact tgaacatgtt 180 tggggggcta gagtgtgggt tgtactttgg gccttgaatt gatcgcacac agcttatatg 240 taagtctctt cgaaatattg gtcatttctg gaacctaaga actccttctg attagtgcat 300 ccctcgctcc cgccctccgc agtaccgaag ttcggactcc ccgattgagc tgttgacttg 360 cggtgtagat ctgcatccat gtcacaaaca tagataccag gtcacacctt ttttgtccac 420 cagatatcca taccaacgcc atgaaaagaa gcacttcgag tctatttcgc tctggtagcc 480 tcatggactc ttcttcgtat ccccgaatac atccaggcac tgcactccct cgcagacgat 540 catcgctccc tcatggcaaa caggtttcta agaacaccag ccgattcgtt tcaagatact 600 cgagatgagg cacgcctaac cgaagcttcc taatccattc gtcttcattc tcatgcatat 660 cgacttgtag atgtatttaa gacctgtact gatgattgca aacagcatcg acctccaatt 720 atttaacacc tcatccaccg cgtactgcta cacctacaaa cgatgtttcc aggcgcacgg 780 attctcgcta cgcttacatt agctcttcac cttttacatg gcactcatgc tgccatcggg 840 cccattgccg acatgtacat cgtcaacgag gacgtctctc ctgacggctt cgctcgttcg 900 tgagtggatc ctcggtgtgc ttttggccac agcacaaatt tattacgcag ggctgtcgtc 960 gctcgctctg tgccggccac agacccgacg cctgcgtcgg tatcggttcc tggcgtcctc 1020 gtgcaaggaa acaaggtaac ccatgttcgc cttcatgaag ccgctcgttt tgctcactct 1080 tcttagggcg ataactttca gctgaacgtt cgcaatcaac tgtcggactc gactatgttg 1140 aagactacta gtatcgtatg catgtgaatg gttgttttgg atgaatactg acttcctgcg 1200 cagcattggc atggcttctt tcaatccggc tctacgtggg cagatggtat gtcttcacta 1260 cgtattacga tgcctcgcta atcttcttca aggacccgcg ttcgtgaatc agtgccccat 1320 cgcctcgggg aatagcttcc tgtgagtgtc cttaccttgt ctctatcttt cattcattga 1380 acattctcag atatgacttt aacgttcccg accaagctgg cacgttctgt aagtcgacga 1440 tcatgaatcc actttgtgcc tcctaatcgt atatgcaggg taccattcgc atctttccac 1500 ccagtattgt gatggtctta gaggaccatt cgtagtgtaa gtttcatatc gataagacgc 1560 taagacgcgc cgggcttaat catccacgca gatacgatcc ctccgatccc cacctgtcct 1620 tatatgacgt tgacaacggt gagctgtgaa cgtattccgg tctctgcaac gtgctgacag 1680 tctcacttcc agccgacact gtcattacac ttgaagattg ggtacatttc actccctctc 1740 cctcatggaa attgatgtgt tcttactttt tctgtagtac cacgttgcgg cccctcaggg 1800 tgcagtgctt cctactcctg atagcacgct catcaatggt aaaggtcgct tcgccggggg 1860 gccgacttcc cctttggcta tcatcaacgt cgaaagcaac aagcgatatc gtttccgact 1920 tatctcgatg tcttgcgacc ccaatttcac gttctcaatc gacggtcact ctttgcaggt 1980 cattgaagca gatgctgtca atattgtgcc catcgtcggt ttgtctttca cgctcggcct 2040 cactgtcccg attcgctgac tatagatggt cgatattgta gtggatagta ttcaaatctt 2100 cgcgggtaag taatacccac cctttcgtca aatctttact aagccgagtt tcaaggccaa 2160 cgctattctt tcgtcctgaa tgccaatcag gctgttgaca attattggat tcgcgcaaat 2220 cccaacttgg gatcgactgg tatggcattt tgaaagcaac acttgtgctt cgctgacttc 2280 ccgtaatgcc aggcttcgaa ggtggtatta attccgctat ccttcggtat gctggtgcca 2340 ctgaaaatga ccctgccacg acttcgtcga cgagcactcc ccttctggag actaatcttg 2400 tgccactcga aaatcctggt gctcctggtc cagctgtccc tggaggcgca gacatcaaca 2460 tcaatcttgc tatgggcttc gactttacta actttgaaat gaccatcaac ggtacgcagt 2520 ctaggtcttt tcaatgcctt ggatggttac tcatctaccc atgcccaggt tcccccttca 2580 aagcaccaac tggtaagccc aactcgccag cgaaatacaa aaaaattgat gtgatattcc 2640 tgtagctcct gttctgctcc agattctgtc aggtgctacg cctgccgctt cgcttcttcc 2700 ttcaggtagt atatacgcgc tagcagccaa caaagtcgtc gaaatctcca tacccgcctt 2760 agctgtcgga ggaccggtaa ggtcaaatcc cagcgccaga aagcatgtgc tgacaactat 2820 cccacctgaa cagcatcctt tccatcttca cggagtgagt aatgcgacac gcacaatttt 2880 ctccagcggc tgatcgacct cgtatagcac acgttcgacg tcatcaggag tgcgggctct 2940 actacgtata acttcgacac ccctgcgcga cgcgatgttg tcaacactgg aattgacgcg 3000 aacgacaacg ttaccatccg ctttgtgacg gataatccgg gcccatggtt cctccactgg 3060 taggcatttc ccgcaattcg tgcgacagcc atagactgac agcttcccct agccacattg 3120 actggcatct cgaaatgtag gtggcattct ttattgattc aattactcga ctcaaaggca 3180 tttagcggtc tcgcggtcgt tttcgccgaa gatgtggcgt ccatcaaggc cccgcctggt 3240 atgcctcttc gtatctatcc accgcagcct gtgcgtatat gctgaccttc atttctccag 3300 ccgcgtggga cgacttgtgt ccgatttatg atgctttgag cgattccgac agaggtggca 3360 tagcttagga tcgcctccct gtctctgtct acttacggaa ataaagtgga aaaaaataga 3420 accatctgca ttctagtcaa ctcattcttt gaagctcgcc 3460 //