ID   VAO_PLEOS      STANDARD;      PRT;    28 AA.
AC   P21800;
DT   01-MAY-1991 (Rel. 18, Created)
DT   01-MAY-1991 (Rel. 18, Last sequence update)
DT   25-JAN-2005 (Rel. 46, Last annotation update)
DE   Veratryl alcohol oxidase (VAO) (EC 1.1.3.-) (Fragments).
OS   Pleurotus ostreatus (Oyster mushroom) (White-rot fungus).
OC   Eukaryota; Fungi; Basidiomycota; Hymenomycetes; Homobasidiomycetes;
OC   Agaricales; Pleurotaceae; Pleurotus.
OX   NCBI_TaxID=5322;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   STRAIN=Florida;
RX   MEDLINE=91120798; PubMed=1991127; DOI=10.1016/0304-4165(91)90190-R;
RA   Sannia G., Limongi P., Cocca E., Buonocore F., Nitti G., Giardina P.;
RT   "Purification and characterization of a veratryl alcohol oxidase
RT   enzyme from the lignin degrading basidiomycete Pleurotus ostreatus.";
RL   Biochim. Biophys. Acta 1073:114-119(1991).
CC   -!- FUNCTION: Plays a central role in the biodegradation of lignin.
CC       Oxidizes veratryl alcohol to veratraldehyde reducing O(2) to
CC       H(2)O(2).
CC   -!- COFACTOR: FAD.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Temperature dependence:
CC         Moderately thermostable. Has a 1.5 h half-life at 55 degrees
CC         Celsius;
CC   -!- MISCELLANEOUS: Primary aromatic alcohols with methoxy substituents
CC       in position four are good substrates for VAO.
DR   PIR; S13664; S13664.
KW   Direct protein sequencing; FAD; Flavoprotein; Glycoprotein;
KW   Lignin degradation; Oxidoreductase.
FT   VARIANT       1      3       Missing (in 60-70% of the mature VAO).
FT   NON_CONS     23     24
SQ   SEQUENCE   28 AA;  2910 MW;  2363DAD466FC8920 CRC64;
     KPTADFDYIV VGAGNAGNVV AYRTVQAL
//