ID   PLMP_GRIFR     STANDARD;      PRT;   167 AA.
AC   P81054;
DT   15-JUL-1998 (Rel. 36, Created)
DT   15-JUL-1998 (Rel. 36, Last sequence update)
DT   25-OCT-2004 (Rel. 45, Last annotation update)
DE   Peptidyl-Lys metalloendopeptidase (EC 3.4.24.20) (MEP) (GFMEP).
OS   Grifola frondosa (Maitake).
OC   Eukaryota; Fungi; Basidiomycota; Hymenomycetes; Homobasidiomycetes;
OC   Agaricales; Schizophyllaceae; Grifola.
OX   NCBI_TaxID=5627;
RN   [1]
RP   PROTEIN SEQUENCE, AND MASS SPECTROMETRY.
RX   MEDLINE=98043693; PubMed=9374478; DOI=10.1074/jbc.272.48.30032;
RA   Nonaka T., Dohmae N., Hashimoto Y., Takio K.;
RT   "Amino acid sequences of metalloendopeptidases specific for acyl-
RT   lysine bonds from Grifola frondosa and Pleurotus ostreatus fruiting
RT   bodies.";
RL   J. Biol. Chem. 272:30032-30039(1997).
CC   -!- CATALYTIC ACTIVITY: Preferential cleavage in proteins: Xaa-|-Lys
CC       (in which Xaa may be Pro).
CC   -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- MASS SPECTROMETRY: MW=18028; METHOD=MALDI; RANGE=1-167;
CC       NOTE=Ref.1.
CC   -!- SIMILARITY: Belongs to the peptidase M35 family.
DR   PDB; 1G12; X-ray; A=1-167.
DR   PDB; 1GE5; X-ray; A=1-167.
DR   PDB; 1GE6; X-ray; A=1-167.
DR   PDB; 1GE7; X-ray; A/B=1-167.
DR   MEROPS; M35.004; -.
DR   InterPro; IPR006025; Pept_M_Zn_BS.
DR   PROSITE; PS00142; ZINC_PROTEASE; FALSE_NEG.
KW   3D-structure; Direct protein sequencing; Glycoprotein; Hydrolase;
KW   Metalloprotease; Zinc.
FT   DISULFID      5     75
FT   DISULFID     77     97
FT   METAL       117    117       Zinc (catalytic) (By similarity).
FT   ACT_SITE    118    118       By similarity.
FT   METAL       121    121       Zinc (catalytic) (By similarity).
FT   CARBOHYD     42     42       O-linked (Man); partial.
FT   STRAND        2      3
FT   HELIX         7     33
FT   HELIX        39     45
FT   HELIX        50     64
FT   TURN         65     65
FT   HELIX        68     70
FT   STRAND       72     74
FT   STRAND       84     85
FT   TURN         88     89
FT   TURN         91     92
FT   STRAND       93     96
FT   HELIX        98    102
FT   TURN        108    109
FT   HELIX       111    121
FT   HELIX       123    125
FT   TURN        126    126
FT   STRAND      129    129
FT   HELIX       134    144
FT   HELIX       146    149
FT   TURN        150    151
FT   STRAND      152    152
FT   HELIX       153    161
SQ   SEQUENCE   167 AA;  18044 MW;  A372825B68EA55BB CRC64;
     TYNGCSSSEQ SALAAAASAA QSYVAESLSY LQTHTAATPR YTTWFGSYIS SRHSTVLQHY
     TDMNSNDFSS YSFDCTCTAA GTFAYVYPNR FGTVYLCGAF WKAPTTGTDS QAGTLVHESS
     HFTRNGGTKD YAYGQAAAKS LATMDPDKAV MNADNHEYFS ENNPAQS
//