ID   RNPO_PLEOS     STANDARD;      PRT;   101 AA.
AC   P81762;
DT   16-OCT-2001 (Rel. 40, Created)
DT   16-OCT-2001 (Rel. 40, Last sequence update)
DT   05-JUL-2004 (Rel. 44, Last annotation update)
DE   Guanyl-specific ribonuclease Po1 (EC 3.1.27.3) (RNase Po1).
OS   Pleurotus ostreatus (Oyster mushroom) (White-rot fungus).
OC   Eukaryota; Fungi; Basidiomycota; Hymenomycetes; Homobasidiomycetes;
OC   Agaricales; Pleurotaceae; Pleurotus.
OX   NCBI_TaxID=5322;
RN   [1]
RP   PROTEIN SEQUENCE, AND CHARACTERIZATION.
RX   MEDLINE=95096021; PubMed=7798182;
RA   Nomura H., Inokuchi N., Kobayashi H., Koyama T., Iwama M., Ohgi K.,
RA   Irie M.;
RT   "Purification and primary structure of a new guanylic acid specific
RT   ribonuclease from Pleurotus ostreatus.";
RL   J. Biochem. 116:26-33(1994).
CC   -!- CATALYTIC ACTIVITY: Two-stage endonucleolytic cleavage to
CC       nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in
CC       G-P with 2',3'-cyclic phosphate intermediates.
CC   -!- ENZYME REGULATION: Inhibited by divalent cations. Inhibition
CC       decreases in the order zinc, lead, cadmium, nickel, mercury.
CC   -!- SIMILARITY: Belongs to the ribonuclease N1/T1 family.
DR   HSSP; P00651; 1RN1.
DR   InterPro; IPR000026; Ribonuc_N1T1.
DR   Pfam; PF00545; Ribonuclease; 1.
KW   Direct protein sequencing; Endonuclease; Hydrolase; Nuclease;
KW   Pyrrolidone carboxylic acid.
FT   MOD_RES       1      1       Pyrrolidone carboxylic acid.
FT   ACT_SITE     36     36       By similarity.
FT   ACT_SITE     54     54       Proton acceptor (By similarity).
FT   ACT_SITE     87     87       Proton donor (By similarity).
FT   DISULFID      7     84       Potential.
FT   DISULFID      9     99       By similarity.
FT   DISULFID     48     82       By similarity.
SQ   SEQUENCE   101 AA;  10789 MW;  FF30477497990D00 CRC64;
     QTGVRSCNCA GRSFTGTDVT NAIRSARAGG SGNYPHVYNN FEGFSFSCTP TFFEFPVFRG
     SVYSGGSPGA DRVIYDQSGR FCACLTHTGA PSTNGFVECR F
//