ID AB050935 standard; genomic DNA; PRO; 7424 BP. XX AC AB050935; XX SV AB050935.2 XX DT 26-JAN-2001 (Rel. 66, Created) DT 27-AUG-2004 (Rel. 81, Last updated, Version 5) XX DE Pseudomonas straminea proT, proH, proA, proL genes, complete and partial DE cds. XX KW . XX OS Pseudomonas straminea OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. XX RN [1] RP 1-7424 RA Maruyama K., Miwa M., Tsujii N., Nagai T., Tomita N., Harada T., RA Sobajima H., Fujita H., Sugisaki H.; RT ; RL Submitted (08-NOV-2000) to the EMBL/GenBank/DDBJ databases. RL Kiyofumi Maruyama, Gifu University, Department of Biomolecular Science, RL Faculty of Engineering; Yanagido 1-1, gifu, Gifu 501-1193, Japan RL (E-mail:maruyama@cc.gifu-u.ac.jp, Tel:81-058-293-3061, Fax:81-058-230-1893) XX RN [2] RX PUBMED; 11826967. RA Maruyama K., Miwa M., Tsujii N., Nagai T., Tomita N., Harada T., RA Sobajima H., Sugisaki H.; RT "Cloning, sequencing, and expression of the gene encoding RT 4-hydroxy-4-methyl-2-oxoglutarate aldolase from Pseudomonas ochraceae RT NGJ1"; RL Biosci. Biotechnol. Biochem. 65(12):2701-2709(2001). XX RN [3] RX PUBMED; 15277747. RA Maruyama K., Shibayama T., Ichikawa A., Sakou Y., Yamada S., Sugisaki H.; RT "Cloning and characterization of the genes encoding enzymes for the RT protocatechuate meta-degradation pathway of Pseudomonas ochraceae NGJ1."; RL Biosci. Biotechnol. Biochem. 68(7):1434-1441(2004). XX CC Pseudomonas ochraceae NJG1 was isolated from soil by its ability CC to grow in a medium containing phthalic acid as a sole carbon CC source. It possessed polar flagella and was an aerobic, CC Gram-negative rod. Based on the conventional taxonomic studies, it CC was identified as Pseudomonas ochraceae. XX FH Key Location/Qualifiers FH FT source 1..7424 FT /db_xref="taxon:47882" FT /mol_type="genomic DNA" FT /note="originally submitted as Pseudomonas ochraceae" FT /organism="Pseudomonas straminea" FT /strain="NGJ1" FT CDS complement(122..1195) FT /codon_start=1 FT /db_xref="GOA:Q9AQI4" FT /db_xref="UniProt/Swiss-Prot:Q9AQI4" FT /note="water dikinase" FT /transl_table=11 FT /EC_number="2.7.9.3" FT /function="Synthesis of selenophosphate from selenide and FT ATP" FT /gene="selD" FT /product="selenide" FT /protein_id="BAB32693.1" FT /translation="MNSDISNISLDATPRLTSLSHGGGCGCKIAPGVLSELLAKSNLPK FT QFFPDLLVGTETADDAAVYKINDEQAIVATTDFFMPIVDDPYDFGRIAATNALSDIYAM FT GGTPLMALAIVGMPINVLPHDVIAKILEGGESVCRDAGIPLAGGHSIDSVEPIYGLVGI FT GIVNPKQMKRNADAKDGDVLILGKPLGVGILSAALKKNLLDDAGYRAMVDATTKLNRPG FT TELAKLDGVHALTDVTGFGLLGHGLELARGAQLSARIDSAKLPMLPGVQALAEQGIFTG FT ASGRNWASYGEHVELASSLTDAQKALLTDPQTSGGLLVSCTQDVVHQVLQVFADSGFAE FT AAVIGRMEAGAPKVFVG" FT CDS 2373..3065 FT /codon_start=1 FT /db_xref="UniProt/TrEMBL:Q9AQI3" FT /evidence=EXPERIMENTAL FT /transl_table=11 FT /function="unknown" FT /gene="proX" FT /product="ProX protein" FT /protein_id="BAB21454.2" FT /translation="MSAALKGISSMATRQVLADLAAAWQEQGGEPVQIESVGGVDAAQR FT VQSGAEAFDVVFLASNAIDKLQAAGRVVAGSKVDLVLSSTAVAVPADAAQPDIGSEEAV FT RAAVLAAPSIGYSTGPSGVALQKLFERWGIADQIQSRIVQARPGVPVGSMIASGEVALG FT FQQLSELIHVEGIRIVGGLPAAIAIDTVFSAGLVAGSAHADAVQRLLAFMASPEAAAAK FT RRQGMEPA" FT CDS 3227..4624 FT /codon_start=1 FT /db_xref="GOA:Q9AQI2" FT /db_xref="UniProt/TrEMBL:Q9AQI2" FT /evidence=EXPERIMENTAL FT /transl_table=11 FT /function="transport of metabolites" FT /gene="proT" FT /product="putative transporter" FT /protein_id="BAB32694.1" FT /translation="MTSQTSKQNTDPTVVNVQTVLNESPFSGFQWGIFFLCFLIVLLDG FT FDTAAIGFIAPSLLGEWGIDKSHLGPVLSAALFGLAFGALGAGPLADRVGRKTVLIIAA FT VVMGGASIASGLVHDLQGLTIWRFITGLGLGAAMPNAITLMNEYCPDSKRSFITNCMFC FT GFPIGSAFGGFIAAWMIPHFGWRSLLIVGGVVPLILAVLMIVMLPESVRFMVLKNYSAD FT RVRKVMVRIAAGTRNAARFVLNDGGAAAAQAQQGPQGLRLVFSQKYLVGTLALWVTYFM FT GLVIVYGLVNWMPVLFKEAGVPASQAAVIAALFQLGGVGAIFVGLLMDRWNANLIIAAG FT YFLTSLGVAFIGQVLGGGVGMLVASVFIAGLLMNAAQSSMQALAAEYYPTECRASGVSW FT MLGIGRFGGIAGSFLVAELSRRHLELPQVFMVVAIPGVIAALALLVKNRYAGGAPRTSM FT LGLGGAH" FT CDS 4665..5693 FT /codon_start=1 FT /db_xref="GOA:Q9AQI1" FT /db_xref="UniProt/TrEMBL:Q9AQI1" FT /evidence=EXPERIMENTAL FT /transl_table=11 FT /EC_number="4.2.1.83" FT /function="reversible hydration of C=C double bond" FT /gene="proH" FT /product="4-Oxalomesaconate hydratase" FT /protein_id="BAB21455.1" FT /translation="MIIDVHGHYTTAPAALGAWRDLQIAGLKDPSKTPSVADLKISDDE FT IRETIETNQLRLMKERGSDLTIFSPRASFMAHHIGDFQTSSTWAAICNELCFRVSELFP FT DHFIPAAMLPQSPGVDPATCIPELVKCVEQYGNVGLNLNPDPSGGHWTSPPLSDKSWYP FT IYEKMVEYDIPAMIHVSTSCNSCFHTTGSHYLNADTTAFMQCLTSDLFKDFPTLKFLIP FT HGGGAVPYHWGRFRGLAQEMKKPLLEEHLLNNIYFDTCVYHQPGINLLTEVIPTKNILF FT ASEMIGAVRGIDPQTGHYYDDTKRYIEATQNLTADEKHAVYEGNARRVFTRLDKALKAK FT GL" FT CDS 5827..6510 FT /codon_start=1 FT /db_xref="GOA:Q9AQI0" FT /db_xref="UniProt/TrEMBL:Q9AQI0" FT /evidence=EXPERIMENTAL FT /transl_table=11 FT /EC_number="4.1.3.17" FT /function="cleavage of C-C bond of hydroxy acid" FT /gene="proA" FT /product="4-Hydroxy-4-methyl-2-oxoglutarate aldolase" FT /protein_id="BAB21456.3" FT /translation="MYELGVVYRNIQRADRAAADGLAALGSATVHEAMGRVGLLKPYMR FT PIYAGKQVSGTAVTVLLQPGDNWMMHVAAEQIQPGDIVVAAVTAECTDGYFGDLLATSF FT QARGARALIIDAGVRDVKTLQEMDFPVWSKAISSKGTIKATLGSVNIPIVCAGMLVTPG FT DVIVADDDGVVCVPAARAVEVLAAAQKRESFEGEKRAKLASGVLGLDMYKMREPLEKAG FT LKYID" FT CDS 6678..>7424 FT /codon_start=1 FT /db_xref="GOA:Q9AQH9" FT /db_xref="UniProt/TrEMBL:Q9AQH9" FT /evidence=EXPERIMENTAL FT /transl_table=11 FT /EC_number="3.1.1.57" FT /function="reversible cleavage of lactone ring" FT /gene="proL" FT /product="2-Pyrone-4,6-dicarboxylate lactonase" FT /protein_id="BAB21457.1" FT /translation="MSQFEKTPGWLDWYANPSKPQFKLPAGAVDAHCHVFGPGNEFPFA FT PERKYTPCDASKAQLYALRDHLGFARNVVVQATCHGADNRAMVDACKSSGGKARGVATV FT KRSISDAELSALHDAGVRGVRFNFVKRLVDFTPKDELMEIAGRIAKLGWHVVIYFEAVD FT LPELWDFFTALPTTVVVDHMGRPDVTKGVDSEEFALFLKFMREHQNVWSKVSCPERLSV FT TGPKALHGEQNAYQDVVPFARRVVEEF" XX SQ Sequence 7424 BP; 1360 A; 2316 C; 2259 G; 1489 T; 0 other; gaattcatca cgcgcctcca tgcagaacgc ccggtctatg ccgggcgttc tgcattcagc 60 gggagatggg agcaggaggg cttttgtccc ttcgtcccgt ttttccgact tccagtcgcg 120 ctcagcccac aaagaccttg ggtgcgcctg cttccatgcg tccaatcact gcggcctcag 180 caaacccgct gtcagcaaag acttggagca cttgatggac tacatcctgg gtacaggaaa 240 ccagcagacc gccagaagtc tgagggtcgg tcagcagtgc tttctgggca tcggtcagag 300 acgaagccag ctcgacatgt tcgccgtagg atgcccagtt gcgtcccgaa gcaccagtga 360 aaatgccttg ctctgcaagc gcttgaacac ccggaagcat aggtagcttg gcgctgtcaa 420 tgcgtgcgct gagctgggcg ccacgggcca gttccaggcc atgacccagc aggccgaatc 480 ccgtcacatc ggtcagcgca tgcacgccgt caagcttggc aagttctgta cctgggcggt 540 tgagcttggt ggttgcatcc accatggcac ggtagcccgc atcgtcgagc aaattcttct 600 tgagcgctgc cgagagaatg cccacgccga ggggcttgcc cagaatcagc acatcgccgt 660 ctttggcatc ggcattgcgc ttcatctgct tggggttgac gatgccgatg ccgaccaggc 720 cgtagatggg ctccaccgaa tcaatcgagt gcccgcccgc cagaggaatg ccggcatcgc 780 ggcagaccga ttcgccgcct tccagaatct tggcgatgac atcgtgcggc aagacattga 840 tgggcatgcc cacgatggcc agcgccatca gcggcgtgcc gcccatggca tagatatcgg 900 acagcgcatt ggtggcggca atgcgaccaa agtcataggg atcgtcgacg atgggcatga 960 aaaagtccgt cgtcgccaca atggcctgct cgtcattgat cttgtaaacg gccgcatcgt 1020 ctgcggtttc cgtgcccacc agcaggtcgg ggaaaaactg cttgggcaga ttgcttttgg 1080 ccagcaactc cgacagcacg ccgggcgcaa tcttgcagcc acagccgccg ccgtgagaca 1140 gagaggtcag gcgcggggtc gcatccaagg aaatgtttga tatatcagag ttcatggcta 1200 taggttaact tgcatgcgta gcaatgctgt accgcctctt aggtataggg atgggagcaa 1260 gcctttatga gagcttgcat cacatgaatc cattttcggc ttcgataaac aagcacggcc 1320 catagtgtgc gatccagtaa agatttgtct gaacgattgt gattcatcat cgctcttgtt 1380 tctggtcagc caaaagcaac cctggcgcgt taaggactga agcgtttccg cgatgaagcg 1440 cgatctcgcc ggccccacgg tgatctatat cgagcatgga gagatatgtc caatcgcact 1500 tcaatcatcc gcagtctggc ggccttggcg ctggtttgcg cagcgggtgc cgcccaggca 1560 caggcctata tcagcggctc catctcgggg cagctggcgc ccggggtcta cggtcaggtc 1620 aatatcggca atgcgccgcc gcagctgctt tatgcccagc ctatgtgggg cggcccggta 1680 gtgccgcagg tgcagcccat ctatatgtgg gtaccgcccg gccattcgcg taactggcgc 1740 cgttactgcg gtcgctacca tgcctgcggc cagccggtct atttcctgcg caatccgcca 1800 ccccattggc gtgcaggacc tccgccccat caccatcacg acaggcgcga ctggcgtgat 1860 gatcgccgcg atcatcgtca ctgggacaga ggcgatcgcc acgaccgacg tgactgggac 1920 aggcatgacc gtgacgaccg ggggcgcggc catggtcacg gccgcggaca tggacgccat 1980 ggtgactaaa ttaggggtta accactaaat tcactgttgc ttatgcacag caataatggc 2040 cgaatgctat tgaaaaaata gcatttggcc ttttctattt attggcttaa cacactgttt 2100 gtaggtattt atacgtgata tgagcaagca ctttgctatg cctttctggc atagctgcta 2160 gcggagggct tgcctagtag gggcgctcgg ggctgccgac aatagtctca ttcgttttga 2220 ctagacggtt gcccttgcgc aaaccctgtc gagcgcccca gtcctgccgg tgtagtgtgc 2280 ccgcagcttg agggagtagc tcgcaaagcc ttgaggctgg gttgcggaat ctggcagggc 2340 agccactgta tccacttcag gagtctttcg gcatgagcgc agcactcaaa ggcatttctt 2400 ccatggcaac ccgccaggtc ctcgccgatc tggcagcagc ctggcaggag cagggcggcg 2460 agccggtgca gatcgaatcc gtgggcggtg tggatgccgc gcagcgcgtg cagtccggtg 2520 cagaggcctt cgatgtggtc ttcctggcct ccaacgccat cgacaagctg caggccgccg 2580 gccgtgtggt tgcaggcagc aaggtggatc tggtgctctc cagcaccgct gtcgccgtgc 2640 ctgccgatgc ggcccagccc gatatcggct cggaggaggc cgtgcgcgct gccgtgctgg 2700 ccgcgcccag catcggttac tccaccggtc ccagcggcgt ggcgctgcaa aagctgttcg 2760 agcgctgggg cattgccgac cagatccagt cccgcatcgt gcaggccagg cccggcgttc 2820 ccgtgggctc catgattgct tcgggagaag tggcgctggg ctttcagcag ctgagcgagc 2880 tgatccatgt cgaaggcatc cgcatcgtgg gcggcctgcc tgccgcgatt gccatcgaca 2940 ccgtgttttc ggccggcctg gtggcgggct cggcccatgc cgatgccgtg cagcgtctgc 3000 tggccttcat ggcttcgcct gaggccgcag cggccaagcg ccgccagggc atggaacccg 3060 cttgaattga ttcgtgtcca ggcgggggcc tgggcactgc accccatcca gcaaaagggc 3120 agtcatagcc ctttcgtgac ggacgtacca gatgcctttg aggctttggc gccagccgcg 3180 agacaggaga tagaggcgac aaagagaggc aactgagatt ccaactatga cttcgcaaac 3240 tagcaaacag aacaccgacc cgacggtcgt caacgtgcag accgtgctca acgagagccc 3300 gttctcgggc tttcagtggg ggattttctt cctctgcttc ttgattgttc tgctcgacgg 3360 ttttgatacc gccgccatcg gcttcatcgc gccttcgctg cttggcgaat ggggcatcga 3420 caaatcccat ctggggccgg tgctgagcgc tgccctgttc ggcctggcgt tcggcgcgct 3480 gggcgcgggt ccgctggcgg accgcgtggg ccgcaagacc gtgctgatca tcgccgcggt 3540 ggtgatgggc ggtgcatcga ttgcttccgg cctggtgcat gacctgcaag gcctgaccat 3600 ctggcgcttc atcaccggtc tgggtcttgg cgccgccatg cccaacgcca tcacgctgat 3660 gaacgaatac tgccctgaca gcaagcgttc cttcatcacc aactgcatgt tctgcggctt 3720 ccccatcggc tcggccttcg gtggctttat cgcagcctgg atgattccgc atttcggctg 3780 gcgcagcctg ctgatcgtgg gcggcgtggt gcccctgatc ctggccgtgc tgatgatcgt 3840 gatgctgccc gagtcggtgc gtttcatggt gctcaagaac tactcggccg accgcgtacg 3900 caaggtgatg gtgcgcatcg ccgcaggcac gcgcaatgcc gcacgcttcg ttctgaacga 3960 cggcggcgcg gctgccgccc aggcacagca gggcccccag ggcctgcgtc tggtgttctc 4020 gcagaaatat ctggtcggca cgctggctct gtgggtgaca tatttcatgg gtctggtgat 4080 cgtctatggc ctggtcaact ggatgcctgt gctgttcaag gaagcgggcg tgcccgccag 4140 ccaggcggcc gtgattgccg cgctgttcca gcttggcggc gtgggcgcga tctttgtcgg 4200 cctgctgatg gaccgctgga atgccaatct gatcattgcg gccggctact tcctgaccag 4260 cctgggcgtg gccttcatcg gccaggtgct cggcggcggc gtgggcatgc tggtggcttc 4320 ggtcttcatt gccggtttgc tgatgaatgc ggcacagtcg tccatgcagg cgctggctgc 4380 cgagtactac cccacggaat gccgcgccag tggtgtgtcc tggatgctgg gcattggccg 4440 ctttggcggc attgcaggct ccttcctggt ggccgagctg tcgcgccgtc acctggagct 4500 gccgcaggtc ttcatggtgg ttgccattcc cggtgtcatc gccgcgctgg cgctgctggt 4560 caagaaccgc tatgccggcg gcgcaccgcg cacctccatg ctgggcctgg gcggcgcgca 4620 ctgaactttt tgatagacga attatttttt aacggagctt gaagatgatc attgacgtac 4680 acggtcacta caccaccgcg cctgcggctc tgggcgcatg gcgcgatctg cagatcgccg 4740 gcctcaagga cccgagcaag accccgtcgg tggccgatct gaagatcagc gacgatgaaa 4800 tccgcgagac catcgaaacc aaccagttgc gcctgatgaa ggagcgtggt tccgatttga 4860 ccatcttcag cccccgtgcc tcattcatgg cgcaccacat cggtgacttc cagacctcca 4920 gcacctgggc cgccatctgc aacgagctgt gcttccgcgt cagcgagctg ttccccgatc 4980 acttcattcc cgccgccatg ctgccccagt cgcccggcgt ggaccctgca acctgcattc 5040 ccgagctggt caagtgtgtg gagcagtacg gcaacgtggg cctgaacctt aaccccgatc 5100 cctcgggcgg tcactggact tcgcccccgc tgtccgacaa gagctggtac cccatctacg 5160 aaaagatggt ggagtacgac atccccgcga tgatccacgt ctccaccagc tgcaattcat 5220 gctttcacac cacgggcagc cactatctga atgccgacac cacggccttc atgcagtgcc 5280 tgacttcgga tctgttcaag gacttcccga ccctgaagtt cctgatcccc catggcggcg 5340 gcgccgtgcc ttaccactgg ggccgcttcc gcggtctggc gcaggagatg aagaagccgc 5400 tgctggaaga gcatctgctc aacaatatct atttcgacac ctgcgtctac caccagcccg 5460 gcatcaacct gctgacggaa gtgattccga ccaagaacat tctgttcgcc agcgaaatga 5520 tcggcgccgt gcgcggcatc gatccgcaga ccggtcacta ctacgacgac accaagcgct 5580 acatcgaagc cacgcagaat ctgacggccg atgaaaagca cgccgtctac gaaggcaatg 5640 cccgccgcgt gttcacgcgc ctggacaagg cgctgaaggc caagggtctg taacttcaca 5700 aaaaagagag cttctatcgc acggtatgaa acgttttcaa tacgaaaacc attcaagtct 5760 gatgatttaa aagcgataac agctcacttt tttgattcaa taacgtcaaa gcaaagaggt 5820 atttccatgt acgaactggg agttgtctac cgcaatatcc agcgcgccga ccgcgctgct 5880 gctgacggcc tggccgccct gggctccgcc accgtgcacg aggccatggg ccgcgtcggt 5940 ctgctcaagc cctatatgcg ccccatctat gccggcaagc aggtctcggg cacggcagtc 6000 accgtgctgc tgcagcccgg cgacaattgg atgatgcatg tggcggccga gcagattcag 6060 cccggcgaca tcgtggtcgc tgccgtcacc gccgagtgca ccgacggcta cttcggcgac 6120 ctgctggcca ccagcttcca ggcgcgcggc gcacgcgcgc tgatcatcga tgccggcgtg 6180 cgcgacgtca agacgctgca ggagatggac tttccggtct ggagcaaggc catctcttcc 6240 aagggcacga tcaaggccac cctgggctcg gtcaacatcc ccatcgtctg cgccggcatg 6300 ctggtcacgc ccggtgacgt gatcgtggcc gacgacgacg gtgtggtctg cgtgcccgcc 6360 gcgcgtgccg tggaagtgct ggctgccgcc cagaagcgcg aaagcttcga aggcgaaaag 6420 cgcgccaagc tggcctcggg cgtcctgggc ctggatatgt acaagatgcg cgagcccctg 6480 gaaaaggccg gcctgaaata tattgactaa cacccctgag tcgcttcgcc tgggcggccc 6540 cgcgccttcc ccctctctcg ctttgcggag ggggacgatg gcctcgctgc gaggcggctc 6600 ttgcatggca tctctgagtt gtgcggcgcc agtattggcg gctacggccc acccagagac 6660 tatcgaggaa aagacaaatg agccaatttg aaaagacccc cggctggctg gactggtacg 6720 ccaaccccag caagccccag ttcaagctgc ctgccggcgc tgtggatgcg cactgccacg 6780 tgttcggccc cggcaacgag ttccccttcg cccccgagcg caagtacacg ccctgcgatg 6840 ccagcaaggc ccagctgtat gcgctgcgcg accatctggg ttttgcgcgc aatgtggtgg 6900 tgcaggccac ctgccacggt gcggacaacc gcgccatggt cgatgcctgc aagtcctcgg 6960 gcggcaaggc ccgcggcgtg gccacggtca agcgctccat cagcgatgcc gagctctcgg 7020 ccctgcacga cgccggcgtg cgcggcgtgc gcttcaactt cgtcaagcgc ctggtggact 7080 tcacgcccaa ggacgagctg atggagatcg cgggccgtat cgccaagctg ggctggcatg 7140 tggtgatcta tttcgaagcc gtggacctgc ccgagctctg ggacttcttc accgcgctgc 7200 ccaccaccgt ggtggtcgac cacatgggcc gccccgacgt caccaagggc gttgacagcg 7260 aggagttcgc cctgttcctg aagttcatgc gcgagcacca gaacgtgtgg agcaaggtgt 7320 cctgccccga gcgtctgtcc gtcaccggcc ccaaggccct tcatggtgag cagaacgcct 7380 accaggacgt ggtgcctttc gcgcgccgcg tggtcgagga attc 7424 //